Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,301,080	T→C	D66G (GAC→GGC)	yhbT ←	SCP2 domain‑containing protein YhbT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,301,080	0	T	C	100.0%	117.1 / NA	35	D66G (GAC→GGC)	yhbT	SCP2 domain‑containing protein YhbT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/13);  total (22/13)

AGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCAAGAACCTGGCGTTTTAGCGCAAATGGCGTCAGTTTTA  >  NC_000913/3300948‑3301215                                                                                                                                     |                                                                                                                                        agCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATc                                                                                                                                  <  1:104075/140‑1 (MQ=255)     tATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGc                                                                                                                              >  2:467855/1‑140 (MQ=255)            aTCTTGTTTACGCGCTGCAATCATCCGCACATCGCTGTCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGaa                                                                                                                       <  1:320825/140‑1 (MQ=255)                 gTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTAACCACCGACGTAAACCGTTGCAGGCAAATAGCACGCACATGAATACtt                                                                                                                  >  2:320825/1‑140 (MQ=255)                  tttACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTa                                                                                                                 >  1:123685/1‑140 (MQ=255)                      cgcgCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCa                                                                                                             >  2:416474/1‑140 (MQ=255)                      cgcgCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCa                                                                                                             >  2:416457/1‑140 (MQ=255)                         gccgcAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCg                                                                                                          <  1:181102/140‑1 (MQ=255)                                        agATCGCTGGCGTCGGCACTAAAACCCACATCAGCTTTCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTc                                                                                           <  1:352141/140‑1 (MQ=255)                                          aTCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGGACATGAATACTTAAACAGCGGTCTTCAAGAAACTCCa                                                                                         >  1:265931/1‑140 (MQ=255)                                          aTCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCa                                                                                         >  1:561395/1‑140 (MQ=255)                                          aTCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCa                                                                                         >  2:416176/1‑140 (MQ=255)                                            cGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCGATATCACGCACATGGAGACTTAACCCGCGGCCTTCAAGAAACTCCAGc                                                                                       >  2:352141/1‑140 (MQ=255)                                            cGCTGGCGTCGGCACTAAAAC‑CACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCt                                                                                      >  1:165528/1‑140 (MQ=255)                                               tGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCg                                                                                    >  2:807771/1‑140 (MQ=255)                                                      ggCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCcat                                                                                <  1:761261/137‑1 (MQ=255)                                                      ggCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCcat                                                                                >  2:761261/1‑137 (MQ=255)                                                         aCTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCTGCTCGCCATCATCCa                                                                          >  2:815530/1‑140 (MQ=255)                                                                       cAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTGAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAAt                                                            >  2:530516/1‑140 (MQ=255)                                                                         gCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTg                                                          >  1:80030/1‑140 (MQ=255)                                                                          cTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGc                                                         >  1:810478/1‑140 (MQ=255)                                                                          cTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGc                                                         <  2:810478/140‑1 (MQ=255)                                                                           ttGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGcc                                                        <  2:68878/140‑1 (MQ=255)                                                                                    cTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCACGCGATCGTCCAGCGCCTGGCGGAATTGCCAGCTTAAGa                                               >  2:292762/1‑140 (MQ=255)                                                                                     tGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGAc                                              <  1:77556/140‑1 (MQ=255)                                                                                      ggCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGAcc                                             <  1:781199/140‑1 (MQ=255)                                                                                            cGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTg                                           >  2:83074/1‑136 (MQ=255)                                                                                            cGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTg                                           <  1:83074/136‑1 (MQ=255)                                                                                            cGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCa                                       >  2:182884/1‑140 (MQ=255)                                                                                            cGACCAGTTTGCCATTCACCACCGAGCTAAACCATTGCAGGCCAATATCACGCACATGAATACTTATCCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCa                                       <  2:491248/140‑1 (MQ=255)                                                                                               ccAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCAAGa                                    >  1:144671/1‑140 (MQ=255)                                                                                                                       tAAACCATTGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCAAGAACCTGGCGTTTTAGCGCAAATGGc            >  1:550109/1‑140 (MQ=255)                                                                                                                       tAAACCATTGCAGGCAAATATCTCGTACAGGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCAAGAACCTGGCGTTTTAGCGCAAATGGc            <  1:292762/140‑1 (MQ=255)                                                                                                                              ttGCAGGGCAATATTCCGCACATGAATACTTAACCAGCGGGCCTCAAGAAAATCCAGCGCGCCATCATCCAACGCCCGGCGGAATTGCCAGCTTAAAACCTGCTCAAGAACCTGGCGGTTTTTCGCCAATGGCGGCAggt     >  2:509755/1‑138 (MQ=255)                                                                                                                               tGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCAAGAACCTGGCGTTTTAGCGCAAATGGCGTCAGttt    <  2:550109/140‑1 (MQ=255)                                                                                                                               tGCAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCAAGAACCTGGCGTTTTAGCGCAAATGGCGTCAGttt    >  2:156865/1‑140 (MQ=255)                                                                                                                                 cAGGCCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCAAGAACCTGGCGTTTTAGCGCAAATGGCGTCAGTTTTa  <  1:571923/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        AGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCGGCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACGCACATGAATACTTAACCAGCGGCCTTCAAGAAACTCCAGCTCGCCATCATCCAGCGCCTGGCGGAATTGCCAGCTTAAGACCTGCTCAAGAACCTGGCGTTTTAGCGCAAATGGCGTCAGTTTTA  >  NC_000913/3300948‑3301215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑