Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,306,288	A→G	F525L (TTT→CTT)	deaD ←	ATP‑dependent RNA helicase DeaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,306,288	0	A	G	100.0%	142.4 / NA	43	F525L (TTT→CTT)	deaD	ATP‑dependent RNA helicase DeaD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/19);  total (24/19)

CTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAACAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGAC  >  NC_000913/3306151‑3306422                                                                                                                                          |                                                                                                                                       cTCACCGCCAGTATGCGGCTGTGCATCACCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAACACTTCACCCGGCATACCTTTCGGCAGTTCGATGGGGGAGTGAGAagcaagca                                                                                                                                      >  2:656628/1‑140 (MQ=255)   cACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAagcagc                                                                                                                                    <  2:712992/140‑1 (MQ=255)        ccAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGAt                                                                                                                               <  1:567681/140‑1 (MQ=255)         cAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGATGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATg                                                                                                                              >  2:437630/1‑140 (MQ=255)              tGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTAcc                                                                                                                         <  2:727127/140‑1 (MQ=255)                      tgCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAAc                                                                                                                 >  1:657739/1‑140 (MQ=255)                        cATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACgg                                                                                                               >  1:644082/1‑140 (MQ=255)                        cATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGGAAAGTGTTGCAGCACCTCACCCGGCATAACTTTCGGCAGCTCGCTGGTGGAGCGAGAAGCAAACAACTTGGTCTTTCCAATGTAAccg                                                                                                               >  2:342578/1‑138 (MQ=255)                              ccAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGctgctg                                                                                                         <  1:462327/140‑1 (MQ=255)                                  tAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGt                                                                                                     >  1:748512/1‑140 (MQ=255)                                       gCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATAACTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCt                                                                                                >  1:476002/1‑140 (MQ=255)                                        cATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGGGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGcc                                                                                                 <  2:430708/138‑1 (MQ=255)                                        cATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGGGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGcc                                                                                                 >  1:430708/1‑138 (MQ=255)                                          tGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGGTGTGACCAATGTAACGGGAGCTGATGTCGCCTTCg                                                                                             >  2:368903/1‑140 (MQ=255)                                            ttCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGtt                                                                                           >  1:62925/1‑140 (MQ=255)                                                tCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCa                                                                                       >  2:736942/1‑140 (MQ=255)                                                  ggCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAAt                                                                                     <  1:736942/140‑1 (MQ=255)                                                      tgttgAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCa                                                                                 >  1:677593/1‑140 (MQ=255)                                                      tgttgAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAAGGTAACGGGTGCTGATGTCGCCTTCGTTAGCAATCGCa                                                                                 >  1:313855/1‑140 (MQ=255)                                                       gttgAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGTTGTTACCACTGTAACGGCTGCTGATATCGCTCTCGTTAGCAATCGCAc                                                                                >  2:310436/1‑140 (MQ=255)                                                        ttgAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCAcc                                                                               <  2:87335/140‑1 (MQ=255)                                                                  gAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGGCTTGGTAAGCAATTGCACCAACGATATGa                                                                     >  1:393122/1‑140 (MQ=255)                                                                  gAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGa                                                                     >  2:90593/1‑140 (MQ=255)                                                                    gTGCGCGTAAAGTGTTGCAGCAGTTTACCTGGCATACGTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACg                                                                   <  1:254618/140‑1 (MQ=255)                                                                    gTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACg                                                                   <  1:42064/140‑1 (MQ=255)                                                                     tGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGa                                                                  >  1:194379/1‑140 (MQ=255)                                                                       cgcgTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACg                                                                   >  2:690604/1‑137 (MQ=255)                                                                       cgcgTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACg                                                                   <  1:690604/137‑1 (MQ=255)                                                                              aGTGTTGCAGCACTTCGCCCGGGATACCTTTTGTCTGTTTGATGGTGGGGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAaca                                                         <  2:393122/140‑1 (MQ=255)                                                                                tgtTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACAcc                                                       <  1:500507/140‑1 (MQ=255)                                                                                  ttGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACcat                                                     >  1:177578/1‑140 (MQ=255)                                                                                      agcaCTTCACCCGGCATACCTTTCGTCAGTTCGATGGAGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCatcatc                                                 <  1:310436/140‑1 (MQ=255)                                                                                       gcaCTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATcg                                                <  2:177578/140‑1 (MQ=255)                                                                                          ctgcACCCGGCATACCTTTCGGCAGTTCGATGGCGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCgg                                             >  2:524976/4‑140 (MQ=255)                                                                                          cTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATcg                                                <  2:780308/137‑1 (MQ=255)                                                                                          cTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATcg                                                >  1:780308/1‑137 (MQ=255)                                                                                          cTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCATTAGCAATAGCAACAAAGATATGACCAACTTCAACACCATCATCGCgg                                             >  2:308615/1‑140 (MQ=255)                                                                                              aCCCGGCATACCTTTCGGCAGTACGGTGGGGGAGTGACAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCa                                         <  1:368903/140‑1 (MQ=255)                                                                                                    cATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACCCCATCATCGCGGCCCACTTCaa                                   <  2:194379/140‑1 (MQ=255)                                                                                                         cTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCgg                              <  1:437630/140‑1 (MQ=255)                                                                                                         cTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCgg                              <  2:657739/140‑1 (MQ=255)                                                                                                            tCGGCAGTTCGATGGTGGAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTAc                           >  2:50861/1‑140 (MQ=255)                                                                                                                        tggtggAGTGAGAAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCg               >  1:60082/1‑140 (MQ=255)                                                                                                                                 gagaAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCa      >  1:564421/1‑140 (MQ=255)                                                                                                                                  agaAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCac     >  2:747503/1‑140 (MQ=255)                                                                                                                                   gaAGCAAGCAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCacg    <  2:564421/140‑1 (MQ=255)                                                                                                                                     agcaagcaGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACGTCAATGCCGTACAGCTGCATAGCGCCAACATCacgac  >  1:355512/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCGATGGTGGAGTGAGAAGCAAACAGCTTGATGTTACCAATGTAACGGCTGCTGATGTCGCCTTCGTTAGCAATCGCACCAACGATATGACGAACTTCAACACCATCATCGCGGCCCACTTCAATGCGGTACAGCTGCATATCGCCAACATCACGAC  >  NC_000913/3306151‑3306422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑