Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,353,504 G→A H183Y (CAC→TAC)  yhcC ← radical SAM family oxidoreductase YhcC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,353,5040GA97.1% 111.2 / NA 34H183Y (CAC→TAC) yhcCradical SAM family oxidoreductase YhcC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/19);  minor base C (0/1);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTGGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCG  >  NC_000913/3353371‑3353637
                                                                                                                                     |                                                                                                                                     
gCTTTCGCCATAATGCTTCCTTTCACAATATCCAGCGGAGGCAGCTTTCTGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAaca                                                                                                                                 <  2:263787/140‑1 (MQ=255)
   ttCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAaca                                                                                                                                 <  2:762364/137‑1 (MQ=255)
   ttCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAaca                                                                                                                                 >  1:762364/1‑137 (MQ=255)
     cGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCt                                                                                                                            >  2:758159/1‑140 (MQ=255)
                 gcCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCt                                                                                                                <  1:471099/140‑1 (MQ=255)
                 gcCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCt                                                                                                                <  1:114488/140‑1 (MQ=255)
                      tCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGt                                                                                                           <  1:677623/140‑1 (MQ=255)
                        cagatatGCAGCGGATGAAGCTTTATGCCGTGCACTCCGTTTTCAACCTCTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGc                                                                                                         <  2:286425/137‑1 (MQ=255)
                           atatGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAg                                                                                                      <  2:591059/140‑1 (MQ=255)
                             atGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCt                                                                                                    <  2:114229/140‑1 (MQ=255)
                              tGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCTAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTg                                                                                                   >  2:461222/1‑140 (MQ=255)
                              tGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCAGACAATTATGTACGAAGACACCTTCGGCCCACGCTGACGTGCCAGCTg                                                                                                   >  2:220821/1‑140 (MQ=255)
                                   cGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTg                                                                                              >  1:55315/1‑140 (MQ=255)
                                      aTGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAAGACAACTTAAGCCCGCGCTGACTTGCCAGCTGGGTTGTAc                                                                                           >  2:309264/1‑140 (MQ=255)
                                      aTGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTAc                                                                                           <  1:779692/140‑1 (MQ=255)
                                      aTGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCACGGTGACGTACCAGCTGGGTTgctg                                                                                           >  1:286425/1‑137 (MQ=255)
                                        gCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGc                                                                                         >  2:46110/1‑140 (MQ=255)
                                             tttATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATa                                                                                    <  2:740476/140‑1 (MQ=255)
                                                 tGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAg                                                                                >  2:638092/1‑140 (MQ=255)
                                                      tCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCaaa                                                                           >  1:541121/1‑140 (MQ=255)
                                                                    aaCCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGt                                                             <  1:758159/140‑1 (MQ=255)
                                                                    aaCCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGt                                                             <  1:47565/140‑1 (MQ=255)
                                                                         cTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTgatg                                                        <  1:46110/140‑1 (MQ=255)
                                                                         cTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTgatg                                                        <  2:541121/140‑1 (MQ=255)
                                                                          tCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTgatgc                                                       <  1:154973/140‑1 (MQ=255)
                                                                            gCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTgatgcga                                                     >  2:694522/1‑140 (MQ=255)
                                                                                                        cccTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTa                         >  2:564342/1‑140 (MQ=255)
                                                                                                        cccTTCGCCAGGCAGGCCGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAAACATGGCCGGGGTGGATGCGATGCAGTGTTTGGTCGTGGGCGGTTTGTa                         >  2:215233/1‑140 (MQ=255)
                                                                                                                      ggcCGACAATTAAGTCGGAACACACATTCAGCCCGCGTTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGc           <  1:309264/140‑1 (MQ=255)
                                                                                                                        ccGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGc           <  1:29908/138‑1 (MQ=255)
                                                                                                                        ccGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGc           >  2:29908/1‑138 (MQ=255)
                                                                                                                        ccGACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCca         <  1:189767/140‑1 (MQ=255)
                                                                                                                          gACAATTAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCcaca       <  2:718446/140‑1 (MQ=255)
                                                                                                                               ttAAGTAGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCg  <  2:55315/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
GCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCAGCGTTTGCAAACATTCAGCCTGCCCTTCGCCAGGCAGGCCGACAATTAAGTGGGAACACACCTTCAGCCCGCGCTGACGTGCCAGCTGGGTTGTACGCTGATAACAGGCAAAATCATGGCCGCGGTTGATGCGATGCAGTGTTTTGTCGTGGGCGGTTTGTAGCCCCAGCTCCAGCCACACTTCG  >  NC_000913/3353371‑3353637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑