Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,358,654 T→C R1310R (CGT→CGC)  gltB → glutamate synthase subunit GltB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,358,6540TC100.0% 75.6 / NA 24R1310R (CGT→CGC) gltBglutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/17);  total (7/17)

ATCAAAGCGTACTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGG  >  NC_000913/3358520‑3358785
                                                                                                                                      |                                                                                                                                   
aTCAAAGCGTACTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGccctcc                                                                                                                                <  1:196317/140‑1 (MQ=255)
    aaGCGTACTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGTCATGGCGAGCGACTAAATCGCCATTCGCCCTCCggtt                                                                                                                            >  1:277708/1‑140 (MQ=255)
     aGCGTACTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCggttg                                                                                                                           >  1:96381/1‑140 (MQ=255)
      gCGTACTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCggttgg                                                                                                                          >  1:84242/1‑140 (MQ=255)
         tACTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTc                                                                                                                       >  1:506770/1‑140 (MQ=255)
          aCTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGGTTTAACGCCATTCGCCCTCCGGTTGGGTcc                                                                                                                      >  2:196317/1‑140 (MQ=255)
          aCTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTcc                                                                                                                      >  1:12117/1‑140 (MQ=255)
           cTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCg                                                                                                                     <  2:425151/140‑1 (MQ=255)
           cTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAAACCCCATTCCCCCCCCCGGTGGTTccc                                                                                                                     >  2:713924/1‑139 (MQ=255)
                 cGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGGCTAAATGACATACGGCCTCCGACTGGTTCCGCCTTcc                                                                                                               >  1:237793/1‑140 (MQ=255)
                 cGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCCCCCGGTGGGTTCGCCCTcc                                                                                                               >  2:53974/1‑140 (MQ=255)
                 cGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCCGCTGAATCGCCCTTCCCCCCCCCGTTGGGTCCCCCCTcc                                                                                                               >  2:340891/1‑140 (MQ=255)
                      ccGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGGGGCTTAATCGCCCTTCCCCCCCCGGTTGGTTCCCCCTTCCCCacc                                                                                                          >  1:669521/1‑138 (MQ=255)
                      ccGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAAACGCCATTAGCCCTCGCGTTGGCTCCGCCTTCCGCAGc                                                                                                          >  1:302377/1‑140 (MQ=255)
                        gCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCa                                                                                                        <  2:12117/140‑1 (MQ=255)
                              cAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGaagc                                                                                                  <  1:49254/140‑1 (MQ=255)
                              cAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTCCCGCAGCCATGaagc                                                                                                  >  1:10071/1‑140 (MQ=255)
                                      cGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTTGCCCTCCCGTTGGGTCCGCCTTCCGCAGCCATGAAACAAGCAttt                                                                                          >  2:49254/1‑139 (MQ=255)
                                      cGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCAt                                                                                                       <  1:139141/127‑1 (MQ=255)
                                      cGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCAt                                                                                                       >  2:139141/1‑127 (MQ=255)
                                           tgtgGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGc                                                                                     >  1:597580/1‑140 (MQ=255)
                                               gAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAaca                                                                                 <  2:96381/140‑1 (MQ=255)
                                               gAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAaca                                                                                 <  1:98086/140‑1 (MQ=255)
                                                aaCGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAacac                                                                                <  1:530607/140‑1 (MQ=255)
                                                       gggggggggAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACAcctgcctg                                                                         <  2:669521/133‑1 (MQ=255)
                                                                  cTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGAc                                                              <  2:815887/140‑1 (MQ=255)
                                                                     tACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGGCTAAACGCCCTTCGCCCTCCCGTTGGTTTCGCCCTCCCCAGGCATGAAGCAAGCATTATCGGCCAACCCCCCCCGTATGGCGCGGCCgg                                                           >  2:576029/1‑140 (MQ=255)
                                                                     tACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCgg                                                           <  2:84242/140‑1 (MQ=255)
                                                                         tGACCGGTGATGCCAACGACTACGTCGGTAACGGCCTGGCGGGCGGCTCAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCggtggt                                                       <  2:237793/140‑1 (MQ=255)
                                                                            ccGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCTTGTATGGCGCGACCGGTGgtcgt                                                    <  2:187654/140‑1 (MQ=255)
                                                                               gTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCAGCCTGTATGGCGCGACCGGTGGTCGTCTg                                                 >  1:574222/1‑140 (MQ=255)
                                                                               gTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAAACGCCCTTCGCCCTCCGGGTGGTTTCGCCCTCCCCCGCCATGAAGCAAACATTATCGGCCACACCCGCCTGTATGGCGCGGCCGCTGggcgtcgg                                                 >  2:467378/1‑138 (MQ=255)
                                                                                                      aaaGGCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGa                          <  1:713924/140‑1 (MQ=255)
                                                                                                         ggCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACg                       >  1:39748/1‑140 (MQ=255)
                                                                                                         ggCATGGCGGGCGGCTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCAGTGGTCGCCTGCATACCGCAGTCCGCGCGGGGGAACg                       >  1:380851/1‑140 (MQ=255)
                                                                                                             tGGCGGGCGGCTTAATCGCCCTTCGCCCCCCGGGTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGGGCGACCGGGGGTAGTCGGTATGCCGGCGGCCTCGCGGGGGAACGTTTc                   >  2:318159/1‑140 (MQ=255)
                                                                                                                   gcggcTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGgcgtg             >  1:744945/1‑140 (MQ=255)
                                                                                                                   gcggcTTAATCGCCATTCGCCCCCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGGAACACCCGCCTGTATGGCGCGGCCGGGGGTCGGCTGTATGCCGCAGGCCGCGCCGGGGGACGTTTTGgcggg             >  2:816343/1‑138 (MQ=255)
                                                                                                                      gcTTAATCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCAGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGgcgtgcgt          <  2:574222/140‑1 (MQ=255)
                                                                                                                            tCGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGGCGTGCGTAACTcc    <  1:576029/140‑1 (MQ=255)
                                                                                                                             cGCCATTCGCCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCg   <  2:744945/140‑1 (MQ=255)
                                                                                                                              gCCATTCGCCCTCCGGTCGGTTACGCCTTCCACAGACATTAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCgg  <  2:277708/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
ATCAAAGCGTACTTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTACCTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATTCGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGCCTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGGCGTGCGTAACTCCGG  >  NC_000913/3358520‑3358785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑