Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 395,726 A→G F188L (TTC→CTC)  ampH ← peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913395,7260AG98.1% 177.3 / ‑4.4 53F188L (TTC→CTC) ampHpeptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (35/17);  total (36/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

CATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGCTGTTCACGGG  >  NC_000913/395589‑395860
                                                                                                                                         |                                                                                                                                      
cATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCg                                                                                                                                      <  1:99735/140‑1 (MQ=255)
       cggcggCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGtt                                                                                                                               >  2:641738/1‑140 (MQ=255)
          cggcACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTaga                                                                                                                            >  1:793058/1‑140 (MQ=255)
                            ggggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGcc                                                                                                          >  2:120552/1‑140 (MQ=255)
                            ggggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCACCAGTGCATCTGCCAGCAGGTCGAGCGCACGGTTAGAGTCCGCCGCCTGACTGcc                                                                                                          >  1:300889/1‑140 (MQ=255)
                                 gtAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTACCAGCAGGGCGAGCGCGAGGTTAGAGTACGCGGCCTGACTGCCTGgcg                                                                                                     >  2:352802/1‑140 (MQ=255)
                                   aGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGcgcg                                                                                                   <  1:426596/140‑1 (MQ=255)
                                   aGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGcgcg                                                                                                   <  1:641738/140‑1 (MQ=255)
                                         tgtCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGGGTACGCCGCCTGACTGCCTGGCGCGGCTTTc                                                                                             >  2:268528/1‑140 (MQ=255)
                                             tttCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCt                                                                                         >  1:623378/1‑140 (MQ=255)
                                                  tCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGcc                                                                                    >  1:22430/1‑140 (MQ=255)
                                                  tCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGcc                                                                                    >  1:22435/1‑140 (MQ=255)
                                                  tCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACGGCATGGCGCAGCTTTCAGCTTCGcc                                                                                    >  1:266191/1‑140 (MQ=255)
                                                       aaTGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGa                                                                               >  1:474626/1‑140 (MQ=255)
                                                                        ctctTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCCCGGCTTTCAGCTTCGCAGTAGAAAGGTATTTCCAGCGtt                                                              >  2:340730/1‑140 (MQ=255)
                                                                           ttCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCGTTCAGCTGCGCGGTAGAAAGGGATTTCCAGCGTTGCt                                                           >  1:260095/1‑140 (MQ=255)
                                                                                 caacTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTcgcgcg                                                     <  1:101156/140‑1 (MQ=255)
                                                                                 caacTGGGTATAAGGCTGGCCGGACGCATTCCCCAGTGCTTCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTcgcgcg                                                     <  2:300889/140‑1 (MQ=255)
                                                                                      gggTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGc                                                <  2:202873/140‑1 (MQ=255)
                                                                                       ggTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGcc                                               >  1:534868/1‑140 (MQ=255)
                                                                                       ggTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGcc                                               >  2:51203/1‑140 (MQ=255)
                                                                                            aaGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACa                                          >  2:10058/1‑140 (MQ=255)
                                                                                              ggCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGc                                                >  1:670724/1‑132 (MQ=255)
                                                                                              ggCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGc                                                <  2:670724/132‑1 (MQ=255)
                                                                                              ggCTTGCCGGACGCACTCGCCACTGTATCTGCCAGCAAGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACaaa                                        <  1:377942/140‑1 (MQ=255)
                                                                                                  tGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTTTTTCCAGCGTTGCTGGCGCGTGGGCCAGACAAACAcc                                    >  1:268009/1‑140 (MQ=255)
                                                                                                  tGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACAcc                                    >  1:86864/1‑140 (MQ=255)
                                                                                                   gCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGc                                                <  2:701421/127‑1 (MQ=255)
                                                                                                   gCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGc                                                >  1:701421/1‑127 (MQ=255)
                                                                                                   gCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCg                                   >  2:768962/1‑140 (MQ=255)
                                                                                                   gCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCg                                   >  2:640005/1‑140 (MQ=255)
                                                                                                   gCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCg                                   >  1:524163/1‑140 (MQ=255)
                                                                                                   gCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCg                                   >  2:468931/1‑140 (MQ=255)
                                                                                                   gCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCATAGAAAGGGATTACCAGCGTTGCTCGCGCGGTGGCCAGACAAACACCg                                   >  2:234619/1‑140 (MQ=255)
                                                                                                         cGCATTCGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGAt                             >  2:105435/1‑140 (MQ=255)
                                                                                                               cGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCcg                       <  2:623378/140‑1 (MQ=255)
                                                                                                               cGCCAGTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCcg                       <  1:10058/140‑1 (MQ=255)
                                                                                                                    gTGCATCTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCa                  <  1:120552/140‑1 (MQ=255)
                                                                                                                      gCATCTGCCAGCAGGTCAAGCGTAAGGTTAGAGTACGCCACCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCAcc                <  2:268009/140‑1 (MQ=255)
                                                                                                                       cATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCg               >  2:608414/1‑140 (MQ=255)
                                                                                                                          cTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGc            >  2:454266/1‑140 (MQ=255)
                                                                                                                          cTGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGc            >  1:666391/1‑140 (MQ=255)
                                                                                                                           tGCCAGCAGGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGCt           <  1:603243/140‑1 (MQ=255)
                                                                                                                           tGCCAGCAGGTCGAGCGCAAGGATAGAGTGCGCCGCCCGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGCt           <  1:268528/140‑1 (MQ=255)
                                                                                                                               agcagGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCggg             <  2:458686/134‑1 (MQ=255)
                                                                                                                               agcagGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCggg             >  1:458686/1‑134 (MQ=255)
                                                                                                                                 cagGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCggg             <  2:741116/132‑1 (MQ=255)
                                                                                                                                 cagGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCggg             >  1:741116/1‑132 (MQ=255)
                                                                                                                                 cagGACGAGAGCAGGGTTAAAGTACGCCGCCTGCCAGCCACGCGCGGCTTTCAGCTTAGACGTAGAAAGGACTTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGCCGATGTCCCGCGGCACCGGGCTGTTCAc     >  2:576221/1‑140 (MQ=255)
                                                                                                                                  agGTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGCTGTTCACg    >  1:165366/1‑140 (MQ=255)
                                                                                                                                    gTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGCTGTTCACggg  >  2:436574/1‑140 (MQ=255)
                                                                                                                                    gTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGCTGTTCACggg  >  1:723254/1‑140 (MQ=255)
                                                                                                                                    gTCGAGCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTCGCCAGACATACACCGGGCGATGTGCCGCGCAACCGGGCTGTTCACggg  >  2:336940/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
CATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGCTGTTCACGGG  >  NC_000913/395589‑395860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑