| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,514,258 | T→C | E44E (GAA→GAG) | gph ← | phosphoglycolate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,514,258 | 0 | T | C | 97.4% | 127.6 / NA | 38 | E44E (GAA→GAG) | gph | phosphoglycolate phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (12/25); minor base A (1/0); total (13/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGAC > NC_000913/3514121‑3514392 | tGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGctc > 1:314222/1‑140 (MQ=255)tGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGctc > 1:632537/1‑140 (MQ=255)tGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGctc > 2:117207/1‑140 (MQ=255)tGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGctc > 2:189658/1‑140 (MQ=255)tGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGctc > 2:459888/1‑140 (MQ=255)tGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGctc < 2:632537/140‑1 (MQ=255)tGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGctc > 2:702852/1‑140 (MQ=255) ttcttcTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCGGTTCCCGATCCAGATAATAACGCGctctt > 1:246208/1‑140 (MQ=255) ttcttcTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGctctt > 1:785262/1‑140 (MQ=255) ttcttcTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGctctt > 1:75579/1‑140 (MQ=255) ctGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCAGCGGCGTTACCAATCCAGGTAGTAACGCGCTCTTCACCt > 2:270107/1‑140 (MQ=255) ccGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCg > 2:432480/1‑140 (MQ=255) ccGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCg < 2:768088/140‑1 (MQ=255) ccGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCg < 2:785262/140‑1 (MQ=255) caACGGGCGGTTTACCCGTTGTTTTACGCTTAGTCTCACTTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAgc < 2:402300/140‑1 (MQ=255) ggCGGTTTACCCATTGTTTTACCCTGAGTCGCACGATCCGGACGCGCCAAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATa < 2:297362/140‑1 (MQ=255) gCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATAc < 2:80839/140‑1 (MQ=255) cGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACa < 1:550078/140‑1 (MQ=255) tttACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAgcg < 1:432480/140‑1 (MQ=255) tACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTCATAACGCGATCTCCACATGCGACGGCCAACACCAGCGCATACAGCGcc > 2:288753/1‑140 (MQ=255) tACCCATTGTTTTACGCTGAGTAGCACGTTCCTGACGCGCCCAGGTCAGTGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGcc < 2:349195/140‑1 (MQ=255) aCCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCa > 2:48882/1‑140 (MQ=255) gTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGcc > 1:435526/1‑132 (MQ=255) gTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGcc < 2:435526/132‑1 (MQ=255) gTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACCCCCGCGCATCCAGCGCCATATCTAc > 1:313544/1‑140 (MQ=255) cTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCgcagcag < 1:189658/140‑1 (MQ=255) tGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCgcagcagc < 2:75579/140‑1 (MQ=255) gAGTCGCACGTTCCTGACGCGCACAGGTCAACGCGAGCTACATCAGAAGATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCgcagcagca < 2:288817/140‑1 (MQ=255) gTCGCACGTTCCTGACGCGCCCTGGTCAACGCGCGCTTCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAg < 1:288753/140‑1 (MQ=255) gTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAg < 2:297440/140‑1 (MQ=255) cGTTCCTGACGCGCCGAGGTCAATGTGCGTTCCATCACAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAgg < 1:270107/140‑1 (MQ=255) ttCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAg < 1:117207/140‑1 (MQ=255) ccTGACGCGCCCAGGTCTATGCCCGCTCCATAAGATCATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCa < 2:246208/140‑1 (MQ=255) ggTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCg < 1:459888/140‑1 (MQ=255) gTCAATGCGCGCTCCATAAGAAGATCTGCGCCGTTACCAATCCAGGTACTAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGt < 2:314222/140‑1 (MQ=255) cATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGTACTGTCGACCAGCGGACCATCGAGATCAAAAGCGAcg > 1:402444/1‑140 (MQ=255) cATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGCCCATCGCACCATCTAGATCAAAAGCGAcg > 1:342083/1‑140 (MQ=255) cATCTGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCACCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGAcg > 1:725260/1‑140 (MQ=255) tGCGCCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGAGCAGGAGCAGTGTAGACCAGCGTACCCTCAAGATCAAAAGCGAcgccgc > 2:239664/1‑140 (MQ=255) cgcCGTTACCAATCCAGGTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGa > 1:683149/1‑140 (MQ=255) gcCGTTACCAATCCACGTAATAGCGAGCTCTTCACCTTCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGaa < 1:305879/140‑1 (MQ=255) aTCCAGGTAATAACGAGCTCTTTACATGCGACGGGCAGCTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCaaa < 1:283813/140‑1 (MQ=255) gTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTAtt < 1:437059/140‑1 (MQ=255) gTAATAACGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCACCGTACCATAAAGATCAGAAGCGACGTCGCGAATATCTTCAAACTTAtt > 2:283813/1‑140 (MQ=255) taataaCGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTc < 2:683149/140‑1 (MQ=255) aaCGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGa > 2:762875/1‑140 (MQ=255) aCGCGCTCTTCACCTTCGACGGGCAACTCCAGCGCATTCAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGAc < 1:399015/140‑1 (MQ=255) aCGCGCTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGAc < 1:702852/140‑1 (MQ=255) | TGTTCTTCTGCCGGAATGTCGTCATCAACGGGCGGTTTACCCATTGTTTTACGCTGAGTCGCACGTTCCTGACGCGCCCAGGTCAATGCGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGAC > NC_000913/3514121‑3514392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |