Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,517,581	T→C	L302L (TTA→TTG)	aroB ←	3‑dehydroquinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,517,581	0	T	C	100.0%	230.5 / NA	66	L302L (TTA→TTG)	aroB	3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/45);  total (21/45)

AACGCCGCTGCGAACTTCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGTAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAACCAATTGCCATACCCC  >  NC_000913/3517446‑3517716                                                                                                                                        |                                                                                                                                        aaCGCCGCTGCGAACTTCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAAcc                                                                                                                                     <  2:113424/140‑1 (MQ=255)         tGCGAACTTCACTCTCACCAATTGCTAACGGAAGAATGAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGc                                                                                                                             <  1:341167/140‑1 (MQ=255)              aCTTCACTCTTACCAGTTGCCACCGGAAGGATTAAGCTCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        <  1:212129/140‑1 (MQ=255)              aCTTCACTCTTACCACTTGTCATCGTAAGGATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        <  1:342483/140‑1 (MQ=255)              aCTTCACTCTTACCAATTGCCAGCGTAAGTATTAAGCCCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        <  1:283929/140‑1 (MQ=255)              aCTTCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        <  1:65934/140‑1 (MQ=255)              aCTTCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        <  1:697758/140‑1 (MQ=255)              aCTTCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        <  2:75471/140‑1 (MQ=255)               cTTCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGc                                                                                                                       <  1:463808/140‑1 (MQ=255)               cTTCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGc                                                                                                                       <  1:785119/140‑1 (MQ=255)                ttCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCa                                                                                                                      <  2:25256/140‑1 (MQ=255)                ttCACTCTCACCAATTATCAACCGAAGAATTAAGCCCATCTCTCCCGCTAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCa                                                                                                                      <  2:397793/140‑1 (MQ=255)                 tCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAg                                                                                                                     <  2:109334/140‑1 (MQ=255)                 tCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAg                                                                                                                     <  1:542483/140‑1 (MQ=255)                 tCACTCTTACCAATTACCAACGTAACAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAg                                                                                                                     <  1:217182/140‑1 (MQ=255)                  cACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAgg                                                                                                                    >  2:622581/1‑140 (MQ=255)                  cACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAgg                                                                                                                    <  1:155660/140‑1 (MQ=255)                   aCTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAggg                                                                                                                   <  2:408769/140‑1 (MQ=255)                   aCTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAggg                                                                                                                   <  1:795814/140‑1 (MQ=255)                     tctTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGAGTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGtt                                                                                                                 <  2:341459/140‑1 (MQ=255)                     tctTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAg                                                                                                                     <  1:87096/136‑1 (MQ=255)                     tctTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAg                                                                                                                     >  2:87096/1‑136 (MQ=255)                        tACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        <  2:21493/130‑1 (MQ=255)                        tACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        >  1:21493/1‑130 (MQ=255)                         aCCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        >  1:527962/1‑129 (MQ=255)                         aCCAATTGCCAACGGAAGAATTAAGCGCACCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAg                                                                                                                        <  2:527962/129‑1 (MQ=255)                          ccAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTataat                                                                                                            >  1:407832/1‑140 (MQ=255)                               tGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAg                                                                                                                     >  1:41792/1‑126 (MQ=255)                               tGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAg                                                                                                                     <  2:41792/126‑1 (MQ=255)                                 ccAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAggg                                                                                                                   <  2:519384/126‑1 (MQ=255)                                 ccAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAggg                                                                                                                   >  1:519384/1‑126 (MQ=255)                                   aaCGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGt                                                                                                   >  2:506638/1‑140 (MQ=255)                                        aagaaTTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCgg                                                                                              <  1:798266/140‑1 (MQ=255)                                        aagaaTTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCgg                                                                                              <  2:185753/140‑1 (MQ=255)                                                aGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTa                                                                                      >  2:758144/1‑140 (MQ=255)                                                     aTCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTg                                                                                 >  2:698819/1‑140 (MQ=255)                                                       ctctcCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCgg                                                                                              >  1:730554/1‑125 (MQ=255)                                                       ctctcCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGcc                                                                               >  2:808066/1‑140 (MQ=255)                                                       ctctcCCGCAAGGACTCTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCgg                                                                                              <  2:730554/125‑1 (MQ=255)                                                         ctcCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCg                                                                             <  1:764664/140‑1 (MQ=255)                                                                   gACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCg                                                                   >  1:566724/1‑140 (MQ=255)                                                                       ttCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGt                                                               <  1:758144/140‑1 (MQ=255)                                                                              cACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGGAGAACTAAACTTCCCGAGACGTTCCGACGTCCGcgcc                                                        >  1:358147/1‑140 (MQ=255)                                                                                cGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCcgc                                                           <  1:634202/135‑1 (MQ=255)                                                                                cGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCcgc                                                           >  2:634202/1‑135 (MQ=255)                                                                                 gcagcaTATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCgccgcc                                                     <  2:407832/140‑1 (MQ=255)                                                                                 gcagcaTATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCgccgcc                                                     <  2:592399/140‑1 (MQ=255)                                                                                  cagcaTATGCGGTAAATGCGCCCGCGCGGACATTTCGCGCGGCCCATTTACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCa                                                    <  2:358147/140‑1 (MQ=255)                                                                                   agcaTATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCAt                                                   <  1:816975/140‑1 (MQ=255)                                                                                             gTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATAccc                                         >  1:430016/1‑140 (MQ=255)                                                                                             gTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATAccc                                         >  2:608075/1‑140 (MQ=255)                                                                                               aaaTACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGc                                       >  1:757772/1‑140 (MQ=255)                                                                                               aaaTACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGc                                       >  2:576824/1‑140 (MQ=255)                                                                                                aaTACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCa                                      <  1:808066/140‑1 (MQ=255)                                                                                                    cGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGa                                  <  1:698819/140‑1 (MQ=255)                                                                                                      ccTGCGCGGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTTCGACGTCCGCGGCGCCATCACCATACCCGCAGCGAcc                                >  1:360564/1‑140 (MQ=255)                                                                                                          cgcgGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCtt                            <  1:803111/140‑1 (MQ=255)                                                                                                          cgcgGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACATTCCGAGGTCCGCGACGCAATGACCATATCCGCAGCGACCGCtt                            >  1:214136/1‑140 (MQ=255)                                                                                                            cgGACATTTCGCGCGGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCa                          <  1:576824/140‑1 (MQ=255)                                                                                                                      gcgcgGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAAcc                <  2:566724/140‑1 (MQ=255)                                                                                                                       cgcgGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAACCa               <  2:688428/140‑1 (MQ=255)                                                                                                                        gcgGCCCATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAACCaa              <  2:411839/140‑1 (MQ=255)                                                                                                                              cATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAACCAATTGCCa        <  1:608075/140‑1 (MQ=255)                                                                                                                              cATTGACCGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAACCAATTGCCa        <  1:622581/140‑1 (MQ=255)                                                                                                                              cATTGACCGGCAACCCACCCCGTTTGAGCAGTGTTTTAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAACCAATTGCCa        <  2:249191/140‑1 (MQ=255)                                                                                                                                 tGACCGGCAACCCAGCCCGCTTTAGCAGGGTTATACTACACTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAACCAATTGCCATAc     <  2:287617/140‑1 (MQ=255)                                                                                                                                    ccGGCAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAACCAATTGCCATAcccc  <  1:506638/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AACGCCGCTGCGAACTTCACTCTTACCAATTGCCAACGGAAGAATTAAGCGCATCTCTCCCGCAAGGACTTTCTTGTCACGCAGCATATGCGGTAAATACGCCTGCGCGGACATTTCGCGCGGCCCATTGACCGGTAACCCAGCCCGCTTGAGCAGGGTTATAATACGCTGCGTTTCGGCAGAACTAAACTGCCCGAGACGTTCCGACGTCCGCGCCGCCATCACCATACCCGCAGCGACCGCTTCACCATGTAACCAATTGCCATACCCC  >  NC_000913/3517446‑3517716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑