| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,518,075 | A→G | S138P (TCC→CCC) | aroB ← | 3‑dehydroquinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,518,075 | 0 | A | G | 97.9% | 159.9 / NA | 47 | S138P (TCC→CCC) | aroB | 3‑dehydroquinate synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (25/21); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCAT > NC_000913/3517946‑3518209 | ccGACGCTAACTCACCCGGGGGAAGCGTTTTCAGACACTCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCTGCCAACGGGGGAATCGAcc < 1:328038/140‑1 (MQ=255) gggAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGtcgtcg > 2:48856/1‑140 (MQ=255) ggAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCgg < 2:51492/140‑1 (MQ=255) gaaaGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCgg < 2:191166/138‑1 (MQ=255) gAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCggg > 1:96281/1‑140 (MQ=255) aGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGAc < 2:124924/140‑1 (MQ=255) tCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGac > 2:826309/1‑140 (MQ=255) tCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGac > 2:1611/1‑140 (MQ=255) tCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCAGCGACAGGAACGTCATCGGGAGTTGAATGAAACGGac > 1:219757/1‑140 (MQ=255) gaTCCACCACCACTG‑AGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACcgc < 1:422656/140‑1 (MQ=255) aTCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACcgc < 2:35802/140‑1 (MQ=255) ccaccacTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGa > 2:451419/1‑140 (MQ=255) cacTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAAc > 2:534837/1‑140 (MQ=255) gAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTcgc < 1:756737/140‑1 (MQ=255) gAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTcgc > 1:200073/1‑140 (MQ=255) aaGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTcgcc > 1:44160/1‑140 (MQ=255) gCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTcgccgc > 1:424753/1‑140 (MQ=255) ggTAGAACGCGCCAATCTTGTTCTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAg < 2:287773/140‑1 (MQ=255) gTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGc > 2:827569/1‑140 (MQ=255) gTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGc > 1:802196/1‑140 (MQ=255) gTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGc > 2:683591/1‑140 (MQ=255) gTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATAAAACGGAAACCGCGTTGATAAGTCGCCGCCGCGAAGc > 1:228296/1‑140 (MQ=255) aGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCg > 2:443346/1‑140 (MQ=255) aCGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTc < 1:443346/140‑1 (MQ=255) cgcgCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCa > 2:475361/1‑140 (MQ=255) cgcCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGa < 2:18626/140‑1 (MQ=255) cgcCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGa < 2:424753/140‑1 (MQ=255) gcCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGAt > 2:716613/1‑140 (MQ=255) gcCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGAt > 2:738398/1‑140 (MQ=255) gcCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGAt > 2:300881/1‑140 (MQ=255) ccAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGAGACCGCTCAGATAACTCGCCGCGACGAAGCCCGTCAGATc > 2:374343/1‑140 (MQ=255) tGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACt < 1:451419/140‑1 (MQ=255) gAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTAcgccgccgc > 1:190627/1‑140 (MQ=255) gAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTAcgccgccgc > 1:151153/1‑140 (MQ=255) aGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACgccgccgcc > 1:487222/1‑140 (MQ=255) ggATGGTTAACCGCACATTTGCCGCCAACGGAGGAATCGTCCTGCGACCGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAg < 2:404498/140‑1 (MQ=255) ggTTGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGcc < 2:151153/140‑1 (MQ=255) ttGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGccac < 1:827569/140‑1 (MQ=255) ttGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGccac < 1:716613/140‑1 (MQ=255) ttGACCGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGccac < 1:475361/140‑1 (MQ=255) cGCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCg < 2:190627/140‑1 (MQ=255) gCAGTTTTGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCgt < 1:300881/140‑1 (MQ=255) gCAGTTTTGCCGCCAACAGGGGCATCGACCTCCGATAGTCACGTCGTCTGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCgt < 2:228296/140‑1 (MQ=255) ttttGCCGCCAACGGGGGAATCGACGTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCgtagta < 1:312822/140‑1 (MQ=255) ttttGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGccacca > 1:725884/1‑132 (MQ=255) ttttGCCGCCAACGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGccacca < 2:725884/132‑1 (MQ=255) aaCGGGGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCAt > 1:643040/1‑140 (MQ=255) | CCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAGCAGGTTGGTAGAACGCGCCAATCATGTTTTTACCGAGGGGATGGTTGACCGCAGTTTTGCCGCCAACGGAGGAATCGACCTGCGACAGTAACGTCGTCGGGACTTGAATGAAACGGACACCGCGCTGATAACTCGCCGCCGCGAAGCCGGTCAGATCGCCCACTACGCCGCCGCCAAGCGCCACCAGCGTAGTATCGCGACCAT > NC_000913/3517946‑3518209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |