Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,525,346	A→G	T826A (ACG→GCG)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,525,346	0	A	G	100.0%	103.1 / NA	30	T826A (ACG→GCG)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/13);  total (17/13)

ATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCC  >  NC_000913/3525211‑3525480                                                                                                                                        |                                                                                                                                       atGAAAGCCGTTCTTTAAGGTGAGCCGGATCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCa                                                                                                                                    <  1:394881/140‑1 (MQ=255)       gCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGac                                                                                                                              >  2:429889/1‑140 (MQ=255)           ttCTTGAAGGTGTGCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCg                                                                                                                                >  2:803026/1‑134 (MQ=255)           ttCTTGAAGGTGTGCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCg                                                                                                                                <  1:803026/134‑1 (MQ=255)                gAAGGTGTGCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCAAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGc                                                                                                                     <  2:568447/140‑1 (MQ=255)                       tgCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGGAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCAc                                                                                                              >  2:112608/1‑140 (MQ=255)                       tgCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCAc                                                                                                              >  2:121438/1‑140 (MQ=255)                                 gcCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGaa                                                                                                    <  2:667452/140‑1 (MQ=255)                                    gCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGa                                                                                                 <  2:6139/140‑1 (MQ=255)                                       gACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCa                                                                                              <  1:154154/140‑1 (MQ=255)                                         cgccgcCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGCCACCACAGGCAGTGCACCAGGTGGGAACGACCAtt                                                                                            >  2:301655/1‑140 (MQ=255)                                                    ggTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATgg                                                                                 <  1:429889/140‑1 (MQ=255)                                                            gACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGcac                                                                         <  1:744959/140‑1 (MQ=255)                                                                   aaTATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAAtt                                                                  >  2:194429/1‑140 (MQ=255)                                                                          atcacagcaCCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGtt                                                               >  2:437492/5‑136 (MQ=255)                                                                          atcaCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGtt                                                               <  1:437492/136‑1 (MQ=255)                                                                             gcagcaCCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTa                                                        >  2:661501/1‑140 (MQ=255)                                                                                    cGGGCAGTTAGCTAATGGTGGCAACAGCCGCGGAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGgc                                                 <  1:112608/140‑1 (MQ=255)                                                                                      ggCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGgcgc                                               >  2:737594/1‑140 (MQ=255)                                                                                      ggCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGgc                                                 <  1:521594/138‑1 (MQ=255)                                                                                      ggCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGgc                                                 >  2:521594/1‑138 (MQ=255)                                                                                           ttAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCgg                                          <  1:121438/140‑1 (MQ=255)                                                                                           ttAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCgg                                          >  2:405724/1‑140 (MQ=255)                                                                                                              gCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGa                       >  2:577753/1‑140 (MQ=255)                                                                                                              gCCGCGAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTAATTAAAAGGGCGCTGCGGCGCGTTTTCAGTTTGCTGa                       >  1:214930/1‑140 (MQ=255)                                                                                                                  cgAAGAGTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAACGACGCTTCGGAGCCTTTTCAGTTTGCTGACaaa                   >  2:349181/1‑140 (MQ=255)                                                                                                                       agTATTTCATCGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCa              <  1:194429/140‑1 (MQ=255)                                                                                                                                 cGAAGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATg    >  2:618261/1‑140 (MQ=255)                                                                                                                                   aaGGTGCGCAGCCGACACAACAGGCAGTGCGCGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGcc  >  2:505014/1‑140 (MQ=255)                                                                                                                                   aaGGTGCGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGcc  >  2:107189/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       ATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGACGCCGCCACCGGGTATTGTGACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCC  >  NC_000913/3525211‑3525480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑