Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	423,327	T→G	C270W (TGT→TGG)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	423,327	0	T	G	97.9%	165.7 / ‑3.9	48	C270W (TGT→TGG)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (23/24);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

ATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGG  >  NC_000913/423194‑423462                                                                                                                                      |                                                                                                                                        aTGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAAc                                                                                                                                   >  2:798959/1‑140 (MQ=255) aTGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAAc                                                                                                                                   >  1:807351/1‑140 (MQ=255)       cGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGaa                                                                                                                             <  2:699657/140‑1 (MQ=255)            ctgcGTTTGCGACACAATACGCAGCAGCTGGTAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGcc                                                                                                                        <  2:317364/140‑1 (MQ=255)                       acacAATACGCAGCACCTGGGACTGTGGCCGGTGCTGCACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACt                                                                                                             <  1:223791/140‑1 (MQ=255)                         acaATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTgg                                                                                                           <  2:180160/140‑1 (MQ=255)                         acaATACGCAGCAGCTGCGAATCCGGCTGGTACTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTgg                                                                                                           <  2:286414/140‑1 (MQ=255)                           aaTACGCAGCAGCTGAGAATGCTGCTGGTTCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTa                                                                                                         <  1:307687/140‑1 (MQ=255)                            aTACGCAGCAGCTGGGAATGTGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTAc                                                                                                        <  2:184022/140‑1 (MQ=255)                                   gcagcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTa                                                                                                 <  2:411596/140‑1 (MQ=255)                                    cagcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACTACCCCGATTCGCCCGGGCGCGGCTGGTACTCGTTTAg                                                                                                >  1:294415/1‑140 (MQ=255)                                     agcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGt                                                                                               >  1:525899/1‑140 (MQ=255)                                     agcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGGGCTTGGCATAACGCCGAATCGCCCTAGCGCCACTGGTACTCGTTTAGt                                                                                               >  2:372099/1‑140 (MQ=255)                                       cTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtga                                                                                             >  1:546381/1‑140 (MQ=255)                                              tGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCAGAACCCCGAGTCGTCCTAGCGCGACTGGTACGCGTTTAGTGATGATGGc                                                                                      >  2:235524/1‑140 (MQ=255)                                               gCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGACTCCCATGCGTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCa                                                                                     <  2:333028/140‑1 (MQ=255)                                                 ggCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACg                                                                                   >  1:825005/1‑140 (MQ=255)                                                         cTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGa                                                                           <  2:501019/140‑1 (MQ=255)                                                         cTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTCATGATGGCACGCCGCTCGa                                                                           <  2:472376/140‑1 (MQ=255)                                                                 cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                                                   >  2:14063/1‑140 (MQ=255)                                                                  gtgtTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttgg                                                                  <  1:14063/140‑1 (MQ=255)                                                                              aGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCtt                                                      >  2:193424/1‑140 (MQ=255)                                                                              aGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCtt                                                      >  1:493145/1‑140 (MQ=255)                                                                                    gATTCCCATGCCTGGTTTGACAGGCATAATCATGGTACGCGTGGTGCTGGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGaa                                                <  2:294415/140‑1 (MQ=255)                                                                                     aTTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAg                                               >  1:726972/1‑140 (MQ=255)                                                                                      ttCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGc                                              <  2:807351/140‑1 (MQ=255)                                                                                       tCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCt                                             <  2:724308/140‑1 (MQ=255)                                                                                            tGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGAtt                                        >  1:803078/1‑140 (MQ=255)                                                                                              ccTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTAt                                      <  1:601402/140‑1 (MQ=255)                                                                                                 ggTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAg                                   >  1:467995/1‑140 (MQ=255)                                                                                                      gACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGa                              <  2:493145/140‑1 (MQ=255)                                                                                                       aCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGaa                             >  1:86681/1‑140 (MQ=255)                                                                                                       aCAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAg                                   <  1:753459/134‑1 (MQ=255)                                                                                                       aCAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAg                                   >  2:753459/1‑134 (MQ=255)                                                                                                         aGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGGTTGGCGGAGCTGGATTATCAGTCGGAAAg                           >  1:314194/1‑140 (MQ=255)                                                                                                                     ggCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGtgaatga               >  2:90376/1‑140 (MQ=255)                                                                                                                      gCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGtgaatgaa              >  1:455670/1‑140 (MQ=255)                                                                                                                      gCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGtgaatgaa              >  1:105548/1‑140 (MQ=255)                                                                                                                         cGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAAtt           >  1:38348/1‑140 (MQ=255)                                                                                                                           ggtggtGCTTGGCACAACCCCGCATGGCCCTGGCGCGTCTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGAATATCAGTCGGAAAGTCTGGTGAATGAAATTTa         <  1:372099/140‑1 (MQ=255)                                                                                                                           ggtggtGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTa         >  1:697567/1‑140 (MQ=255)                                                                                                                            gtggtgCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTAt        >  1:94327/1‑140 (MQ=255)                                                                                                                             tggtgCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATc       <  2:467995/140‑1 (MQ=255)                                                                                                                             tggtgCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATc       <  2:38348/140‑1 (MQ=255)                                                                                                                             tggtgCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATc       <  2:94327/140‑1 (MQ=255)                                                                                                                                tgCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATcgcg    >  2:653594/1‑140 (MQ=255)                                                                                                                                  cTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCggg  <  1:193424/140‑1 (MQ=255)                                                                                                                                  cTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCggg  <  2:525899/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        ATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGG  >  NC_000913/423194‑423462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑