Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,595,404	A→G	V26A (GTG→GCG)	livM ←	branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,595,404	0	A	G	100.0%	87.0 / NA	29	V26A (GTG→GCG)	livM	branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/10);  total (19/10)

CCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGA  >  NC_000913/3595278‑3595537                                                                                                                               |                                                                                                                                      ccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGAc                                                                                                                          >  1:151735/1‑140 (MQ=255)   tttCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTAGTGCCATACAGCTCTAGTTGCGCACCCATAAAGAcgc                                                                                                                        >  1:274055/1‑140 (MQ=255)     tCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACGACCAGTTTAGTGGCATGCAGCTCCATTTGCGCGCCCATAAAGAcgccc                                                                                                                      >  2:218313/1‑140 (MQ=255)     tCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGGGCCATCCTGCTTCAGTTGCGCGCCCATAAAGAcgccc                                                                                                                      >  1:390617/1‑140 (MQ=255)     tCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGGGCCATCCTGCTACACTTGCGCGCCCATAAAGAcgccc                                                                                                                      >  1:240143/1‑140 (MQ=255)      cTGAAAAGCCGGTCACAAAAGGCGGTAAACAAAGCCCCCCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGAcgcccg                                                                                                                     <  1:218313/140‑1 (MQ=255)       ggaaaaGCGGGTCGAAAAAGGGGAAAAAAAAAGCCCCCCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGAcgcccgc                                                                                                                    <  2:662247/137‑1 (MQ=255)              ccGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGcaca                                                                                                             >  2:492511/1‑140 (MQ=255)                             gaaaaaaaaaGACCCCCTCCGTCCCGATAAACCCCTCCTCCCAACGGAGATCCGAAGCCGTGTCGCCCCCCAGTTTGGTGCCATCCAGCTCCAGTTGCGCCCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCa                                                                                              <  2:274055/135‑1 (MQ=255)                                     agcccccccgccgTGCCGATAAACACCCACTCCCAACGAACATCCGAAGCCGTGTCGCCCACCAGTTTGGTGCCACCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGagcag                                                                                      <  2:151735/133‑1 (MQ=255)                                        caccccgccgTGCCGATAAACCCCCCCTGCCAACGGACATCCGAACCCGTGTCGCCCACCAGTTTGGTGCCCCCCAGCTCCACTTGCGCGCCCAAAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAgcgc                                                                                   <  2:816168/136‑1 (MQ=255)                                                  tGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCACCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAata                                                                         <  2:492483/140‑1 (MQ=255)                                                       aTAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCAt                                                                    <  2:165540/140‑1 (MQ=255)                                                       aTAAACACCCACTGCCAACGGAAATTCGAAGCCGTGTGGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGCCGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCAt                                                                    <  1:261277/140‑1 (MQ=255)                                                        aaaaCCCCCCCCGCCAGTGAACGTCCGAAGCCGTGACGACCACCAGTTTGGTGCCACCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATc                                                                   <  2:227454/139‑1 (MQ=255)                                                              cccACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTc                                                             >  2:194256/1‑140 (MQ=255)                                                                 aCTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATa                                                          <  1:492511/140‑1 (MQ=255)                                                                               tCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGCGAGCAGCGCCATTGCACGATGCATCAGTTTCATACTTTCGCTACCTcc                                            >  2:376617/1‑140 (MQ=255)                                                                               tCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTtct                                                    >  2:98573/1‑132 (MQ=255)                                                                               tCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTtct                                                    <  1:98573/132‑1 (MQ=255)                                                                               tCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTcc                                            >  2:741395/1‑140 (MQ=255)                                                                                  aaaGCCGTGCCGACCCCAAGTGTGGTCCCACACAGACCCAGTTGCGCCCCCATAAAGACCCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCggg                                         <  2:369602/139‑1 (MQ=255)                                                                                       cGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGAcc                                    <  1:194256/140‑1 (MQ=255)                                                                                        gtgtCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGAccc                                   >  1:555079/1‑140 (MQ=255)                                                                                          gtCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGGATCGGTTGCATACTTTCTCTAGCTCCGGGCGACCCAg                                 >  1:242089/1‑140 (MQ=255)                                                                                          gtCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAg                                 >  1:592715/1‑140 (MQ=255)                                                                                          gtCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAg                                 >  1:37024/1‑140 (MQ=255)                                                                                               ccaccaGTTTGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATAc                            >  2:489100/1‑140 (MQ=255)                                                                                                      tttGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATCCTTTCTCGACCTCCAGGCGACTCAGAATACCGGTCgg                     >  1:365175/1‑140 (MQ=255)                                                                                                       ttGGTGCCATCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGc                    <  1:419042/140‑1 (MQ=255)                                                                                                               aTCCAGCTCCAGTTGTGCGCCCGTACAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACcag            <  1:376617/140‑1 (MQ=255)                                                                                                               aTCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACcag            >  1:702228/1‑140 (MQ=255)                                                                                                               aTCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACcag            <  2:745395/140‑1 (MQ=255)                                                                                                                tCCAGCTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACcagc           >  2:128502/1‑140 (MQ=255)                                                                                                                    gCTCCAGTTGCGCGCCCTTAAATACGCCCGCCAGCCCAACGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAgcagca       <  2:240143/140‑1 (MQ=255)                                                                                                                     cTCCAGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCAc      >  2:738991/1‑140 (MQ=255)                                                                                                                         aGTTGCGCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGa  <  1:738991/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                      CCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGA  >  NC_000913/3595278‑3595537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑