Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	445,425	A→G	R414R (CGT→CGC)	yajR ←	putative major facilitator superfamily transport protein YajR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,425	0	A	G	100.0%	127.9 / NA	37	R414R (CGT→CGC)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/21);  total (16/21)

ATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAA  >  NC_000913/445288‑445556                                                                                                                                          |                                                                                                                                    aTTCGCCGCGTTTTTTACGCCTCACGAATTGCCTGCTCTCTCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCg                                                                                                                                   <  2:223889/140‑1 (MQ=255)  ttCGCCGCGTTTTTTCTGCCTGGCGCATTCCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAgcgc                                                                                                                                  <  1:225994/140‑1 (MQ=255)  ttCGCCGCGTTTTTTATGCATGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAgcgc                                                                                                                                  <  2:482779/140‑1 (MQ=255)      ccgcGTTTTTTATGCCTTACGAATTGCCTGTTCTAACTCAAAGCGATTTGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACtt                                                                                                                              <  2:346307/140‑1 (MQ=255)          gTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTaa                                                                                                                          >  2:36516/1‑140 (MQ=255)                             ttGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCa                                                                                                          <  1:165630/137‑1 (MQ=255)                             ttGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCa                                                                                                          >  2:165630/1‑137 (MQ=255)                               gCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTGGCAAGCGGACTGTTGACGCCTAGTTTGCGGCAATGtt                                                                                                     >  1:397934/1‑140 (MQ=255)                                  tGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGc                                                                                                  <  1:173374/140‑1 (MQ=255)                                  tGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGc                                                                                                  <  2:784582/140‑1 (MQ=255)                                        aTCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGAt                                                                                            >  2:585603/1‑140 (MQ=255)                                          ctcAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGAttt                                                                                          >  2:81787/1‑140 (MQ=255)                                              aaGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAATGCCGCGTTTGCGACAATGATCGCCGGAATTTCAAt                                                                                      >  2:380137/1‑140 (MQ=255)                                              aaGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAAt                                                                                      >  1:595307/1‑140 (MQ=255)                                              aaGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAAt                                                                                      >  1:736688/1‑140 (MQ=255)                                                   aTTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCa                                                                                 <  2:736688/140‑1 (MQ=255)                                                   aTTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCa                                                                                 <  1:781436/140‑1 (MQ=255)                                                   aTTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCa                                                                                 >  2:443035/1‑140 (MQ=255)                                                   aTTCGTCACTTTGCTGTCGATTCTCTCATTAGCTGAAGGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCa                                                                                 <  1:280109/140‑1 (MQ=255)                                                   aTTCGTCACTTTGCTATCGATTTTCCCATCAGCTGAACGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCa                                                                                 <  1:220404/140‑1 (MQ=255)                                                                   tCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATAcgg                                                                 <  1:502127/140‑1 (MQ=255)                                                                      aTTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATAcggcgg                                                              >  2:430420/1‑140 (MQ=255)                                                                       ttttCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGt                                                             <  1:501416/140‑1 (MQ=255)                                                                       ttttCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGt                                                             <  1:430420/140‑1 (MQ=255)                                                                              aTAAGCTGGATGTTCTTTTTCTCCAATCAAGCCTTCTTTGATGCCTTCTGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCa                                                      <  2:397934/140‑1 (MQ=255)                                                                                          ttcttcttcTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGa                                          <  1:36516/140‑1 (MQ=255)                                                                                             ttcttctGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTg                                       >  1:696869/1‑140 (MQ=255)                                                                                              tcttctGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGt                                      <  2:763656/140‑1 (MQ=255)                                                                                                ttctGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTa                                                 >  1:761881/1‑127 (MQ=255)                                                                                                ttctGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTa                                                 <  2:761881/127‑1 (MQ=255)                                                                                                        tCAACACTTCTTTGACGCCTTCTGTATCTAGCAAGCGAACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACt                            <  1:380137/140‑1 (MQ=255)                                                                                                            cacTTCTTTGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCgg                        >  1:179583/1‑140 (MQ=255)                                                                                                              cttcttGGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGcc                      <  2:688849/140‑1 (MQ=255)                                                                                                                  tttGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCcaca                              >  2:142427/1‑128 (MQ=255)                                                                                                                  tttGATGCCTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCcaca                              <  1:142427/128‑1 (MQ=255)                                                                                                                         ccTTCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGcc           >  1:430385/1‑140 (MQ=255)                                                                                                                           ttCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTTATTGGACTGGCGGCTGTCAGCCACACTGCGGCCAGCATTTCGCCAg         >  2:124568/1‑140 (MQ=255)                                                                                                                            tCAGTTTCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGc        <  1:605922/140‑1 (MQ=255)                                                                                                                                  tCTAGCAAGCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGaaa  <  1:124568/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    ATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAA  >  NC_000913/445288‑445556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑