Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,840,749	T→C	T331A (ACC→GCC)	nepI ←	purine ribonucleoside exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,840,749	0	T	C	100.0%	106.3 / NA	33	T331A (ACC→GCC)	nepI	purine ribonucleoside exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/12);  total (21/12)

CCAATATTATCCAGCGCATAACCGCCGATTGCCGCGCCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGTGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTCACAGCCAGTATTAACGGCG  >  NC_000913/3840637‑3840880                                                                                                                 |                                                                                                                                    ccAATATTATCCAGCGCATAACCGCCGATTGCCGCGCCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAAc                                                                                                          <  1:462463/140‑1 (MQ=255)          tCCAGCGCATAACCGCCGATTGCCGCGCCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCaaa                                                                                                 >  1:681430/1‑140 (MQ=255)               cgcATAACCGCCGATTGCCGCGCCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTa                                                                                            >  2:433374/1‑140 (MQ=255)               cgcATAACCGCCGATTGCCGCGCCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTa                                                                                            >  2:507829/1‑140 (MQ=255)               cgcATAACCGCCGATTGCCGCACCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGGTCCACGTAGACCAGCCCACGGAAACCAATGCAAAAGTTa                                                                                            >  2:253304/1‑140 (MQ=255)               ccaaTACCCGCCGATTGCCGCGCCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTa                                                                                            <  2:65252/137‑1 (MQ=255)                 cATAACCGCCGATTGCCGCGCCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGc                                                                                          >  2:110463/1‑140 (MQ=255)                     aCCGCCGATTGCCGCCCCACAGATACTAGAAAGCTGACTAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGccccc                                                                                      <  1:211883/140‑1 (MQ=255)                           gATTGCCGCGCCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATaa                                                                                >  1:698888/1‑140 (MQ=255)                                                     gCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTa                                                      >  1:24673/1‑140 (MQ=255)                                                     gCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTa                                                      >  2:810101/1‑140 (MQ=255)                                                     gCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTa                                                      >  2:704446/1‑140 (MQ=255)                                                     gCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGAAAATCGCCACGCCGGTAGCAACGATTTTa                                                      >  2:177014/1‑140 (MQ=255)                                                     gCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAAAGCAAAAGTAAGCCCACAGATAATCGACACGCCGGTAGCAACGATTTTa                                                      >  2:357859/1‑140 (MQ=255)                                                     gCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTACCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTa                                                      >  2:37657/1‑140 (MQ=255)                                                      cTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAACGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGACCCCAGACAATCGCCACGCCGGTAGCAACGATTTTAt                                                     >  1:330734/1‑140 (MQ=255)                                                        gAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCg                                                   >  2:609722/1‑140 (MQ=255)                                                         aaTAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGc                                                  <  1:766847/140‑1 (MQ=255)                                                           tAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCtt                                                <  1:507829/140‑1 (MQ=255)                                                                gCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCca                                           >  2:823489/1‑140 (MQ=255)                                                                 ccACCTGAATAGACCGGGCTTTTTCTGCCCGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCcac                                          <  1:357859/140‑1 (MQ=255)                                                                  cACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCcaca                                         <  1:110463/140‑1 (MQ=255)                                                                         aTAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAg                                  <  1:810101/140‑1 (MQ=255)                                                                               ccGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCaa                            >  1:180680/1‑140 (MQ=255)                                                                                  gCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGc                         >  2:651747/1‑140 (MQ=255)                                                                                  gCTTTTTCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGc                         >  2:825598/1‑140 (MQ=255)                                                                                       ttCTGCCTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTca                    <  1:825598/140‑1 (MQ=255)                                                                                         cTGCCTGATCGGCCATCGAGCGTGCTATCCACGTTGATCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTcaca                  <  1:376442/140‑1 (MQ=255)                                                                                            ccTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTCACAGcc               <  2:24673/140‑1 (MQ=255)                                                                                            ccTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAAGCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTCACAGcc               <  1:433374/140‑1 (MQ=255)                                                                                             cTGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAACAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTCACAGCCa              >  2:202738/1‑140 (MQ=255)                                                                                              tGATCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTCACAGCCAg             <  1:704446/140‑1 (MQ=255)                                                                                                aTCGGCCAGCGAGCGGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTCACAGCCAGTa           >  2:714256/1‑140 (MQ=255)                                                                                                         cgagcgGGCGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTCACAGCCAGTATTAACGgcg  >  1:193025/1‑140 (MQ=255)                                                                                                                 |                                                                                                                                    CCAATATTATCCAGCGCATAACCGCCGATTGCCGCGCCACAGGTATTAGCAAGCTGAATAACCGCCACCTGAATAGACCCGGCTTTTTCTGCCTGATCGGCCAGCGAGCGGGTGATCCACGTTGACCAGCCGACGGGAACCAATGCAAAAGTTAGCCCCCAGATAATCGCCACGCCGGTAGCAACGATTTTATCGCTTCCCCACAACGTCAGTACCAACGCACTCACAGCCAGTATTAACGGCG  >  NC_000913/3840637‑3840880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑