Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,844,614	T→C	G217G (GGT→GGC)	adeD →	adenine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,844,614	0	T	C	100.0%	90.3 / NA	27	G217G (GGT→GGC)	adeD	adenine deaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

CTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGTTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTG  >  NC_000913/3844477‑3844744                                                                                                                                          |                                                                                                                                   cTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGctt                                                                                                                                  >  2:423149/1‑140 (MQ=255) cTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCAGTTCGCCAGCTGTCGGTGGACGGTCACTGCCCGGGctt                                                                                                                                  >  1:256409/1‑140 (MQ=255)   cGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTgg                                                                                                                                >  2:202289/1‑140 (MQ=255)         gCGGGACCATCCGCACGTTACCTGCATTGCAGAAATGATGTACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTa                                                                                                                          <  1:386828/140‑1 (MQ=255)                 aTCCGCAGGTTACCGGCTTTGCCGAAATGATTGACCACCCTGGCGTAAGTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACtt                                                                                                                  <  2:256409/140‑1 (MQ=255)                   ccGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCGCCTGACGCTGGAGGGTTACTGCCCGGGCTTTCGTGGTAACGAACTTaa                                                                                                                >  2:374380/1‑140 (MQ=255)                    cGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAAc                                                                                                               <  1:423149/140‑1 (MQ=255)                    cGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAAc                                                                                                               <  1:541745/140‑1 (MQ=255)                      caagTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGc                                                                                                             <  2:733952/137‑1 (MQ=255)                      cAGGTTACCGGCCTTGCTGAAACGATGGACTCCCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGc                                                                                                             <  2:283652/140‑1 (MQ=255)                       aGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGcc                                                                                                            >  2:422223/1‑140 (MQ=255)                            aCCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTatat                                                                                                       >  1:90311/1‑140 (MQ=255)                               ggCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTAc                                                                                                    <  1:19045/140‑1 (MQ=255)                                  cTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGc                                                                                                 <  1:560397/140‑1 (MQ=255)                                           atgatgGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGGAAACAACTTAACGCCTACATTACTGCGGGTATTGa                                                                                        >  2:270655/1‑140 (MQ=255)                                                ggACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACt                                                                                   >  1:169253/1‑140 (MQ=255)                                                                aTTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCa                                                                   >  1:638617/1‑140 (MQ=255)                                                                aTTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCa                                                                   >  1:57919/1‑140 (MQ=255)                                                                aTTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCa                                                                   >  2:539618/1‑140 (MQ=255)                                                                                      tCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGaaa                                             >  2:198048/1‑140 (MQ=255)                                                                                       cGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAAt                                            <  2:57919/140‑1 (MQ=255)                                                                                          tAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTAc                                         <  2:638617/140‑1 (MQ=255)                                                                                             aCTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAAc                                      <  1:144271/140‑1 (MQ=255)                                                                                               tGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTc                                    >  1:820626/1‑140 (MQ=255)                                                                                               tGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTc                                    >  2:515773/1‑140 (MQ=255)                                                                                                   tGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCa                                >  1:719213/1‑140 (MQ=255)                                                                                                   tGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCa                                <  2:719213/140‑1 (MQ=255)                                                                                                      aTTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGt                             <  1:515773/140‑1 (MQ=255)                                                                                                            ccACCTGAACGTGGACGGTCACTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCAGGAAATTACAACTCGGCATGTCGttaa                       <  2:90311/140‑3 (MQ=255)                                                                                                             cACCTGACGCTGGACGGTCACTGCCCGGGCTGGGGCGGTAAAGAACTTCACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTtgat                      <  2:342733/140‑1 (MQ=255)                                                                                                                                 cTGCCCGGGCTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTg  <  1:513782/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   CTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCACTGCCCGGGTTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTG  >  NC_000913/3844477‑3844744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑