| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,875,866 | T→C | C143C (TGT→TGC) | yidX → | putative lipoprotein YidX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,875,866 | 0 | T | C | 98.4% | 205.1 / NA | 61 | C143C (TGT→TGC) | yidX | putative lipoprotein YidX |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (31/29); minor base A (0/1); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAG > NC_000913/3875729‑3875990 | gCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCAc > 1:691159/1‑140 (MQ=255)gCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCAc > 2:798457/1‑140 (MQ=255)gCCGATGACGCAAGATTTGTTTAAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCAc > 1:583674/1‑140 (MQ=255) cGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCa > 1:425515/1‑140 (MQ=255) cGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCa > 1:788480/1‑140 (MQ=255) aCGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCATGTCa > 2:767336/1‑140 (MQ=255) aCGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGt > 1:87979/1‑138 (MQ=255) aCGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGt < 2:87979/138‑1 (MQ=255) aCGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCa > 2:22700/1‑140 (MQ=255) ttttAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTa < 2:118179/140‑1 (MQ=255) ttAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTaaa < 1:437778/140‑1 (MQ=255) tAAATGCGGGGAAAACGAAATTTACCGGCACAATATCCCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTaaaa < 1:319195/140‑1 (MQ=255) aaaTGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAg > 2:804479/1‑140 (MQ=255) aaTGCGGGGAAAAAGAAATTTACCCGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAgg > 2:599272/1‑140 (MQ=255) tGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGca < 2:722218/140‑1 (MQ=255) tACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATa < 2:101685/140‑1 (MQ=255) gCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAAc < 1:207994/140‑1 (MQ=255) agatatCGCTTTACTACGACTGAAATAACGATGAAAAAATGGCACTGGAAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATg < 2:212845/138‑1 (MQ=255) atatCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGa < 1:5202/140‑1 (MQ=255) atatCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGa < 1:599272/140‑1 (MQ=255) cGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTAc < 2:30446/140‑1 (MQ=255) tactactacTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAAc > 2:781582/1‑126 (MQ=255) tactactacTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAAc < 1:781582/126‑1 (MQ=255) tactactacTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCaaa > 2:517875/1‑140 (MQ=255) ctactactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACGATCCATGAGAAGAATAAAAACATGGATTACTCaaact > 1:212845/1‑138 (MQ=255) ctactactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAgg > 2:154115/1‑140 (MQ=255) ctactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGa < 1:549638/140‑1 (MQ=255) ctactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGa < 2:425515/140‑1 (MQ=255) ctactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGa < 1:154115/140‑1 (MQ=255) tactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGAt > 1:758614/1‑140 (MQ=255) actGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATg < 1:22700/140‑1 (MQ=255) ctGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAATAAAATGGATGACTCAAAGGTGATgg > 1:337473/1‑140 (MQ=255) ctGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATgg > 2:91350/1‑140 (MQ=255) ctGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCAGGAGAAAAATAAAAAAATGGATTACTCAAAGGTGATgg > 1:370221/1‑140 (MQ=255) ctGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCAATGCGAAAAATAAAAAAATGGATTACTCAAAGGTGATgg > 2:282708/1‑140 (MQ=255) tGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGc < 1:202698/140‑1 (MQ=255) ggAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCg < 1:517875/140‑1 (MQ=255) aTAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTc > 1:412296/1‑140 (MQ=255) aCGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATTAAACCATGGATTACTCATAGGTGATGGCGTTCTAc > 2:313167/1‑140 (MQ=255) aCGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAc > 2:467337/1‑140 (MQ=255) cGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAcc > 2:414914/1‑140 (MQ=255) gaagaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAcca < 2:194031/140‑1 (MQ=255) gaagaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAAGTGATGGCGTTCTAcca < 2:583674/140‑1 (MQ=255) agaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccatc < 1:467337/140‑1 (MQ=255) agaagaaAAGGCACTGGAAACGCATTATGGTTTTGCCTGAGGTGTTCAGCACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccatc < 2:337473/140‑1 (MQ=255) gaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccatcc > 2:532595/1‑140 (MQ=255) aaaGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTa > 1:722870/1‑140 (MQ=255) aCTGGCAATGCATTATAGTTTTTCCTGTGGTCTTCAACAATGCACCAGATCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGc < 2:370221/140‑1 (MQ=255) aCTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGc > 1:79875/1‑140 (MQ=255) cGCATTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTAt > 2:493350/1‑140 (MQ=255) aTTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGaa < 1:532595/140‑1 (MQ=255) aTTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGaa < 2:758614/140‑1 (MQ=255) aTTATGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGaa < 1:804479/140‑1 (MQ=255) ttATGGTTTTGCCTGTGGTGTTCAACACTGATCTAGGTCGCTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAAt < 2:382863/140‑1 (MQ=255) aTGGTTTTGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATAc > 1:792088/1‑140 (MQ=255) ttttGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTAtt < 2:171704/140‑1 (MQ=255) tGCCTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATCTAATGTACGATTTGATGAATACTATTCAc > 1:382863/1‑140 (MQ=255) ccTGTGGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCAccc > 2:27914/1‑140 (MQ=255) tgtgGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGTGTTCTACCATCCGTTTAAAGTGCGAGTTTAGGAATACTATTCACCCag > 2:297219/1‑140 (MQ=255) tgtgGTGTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCag > 1:648211/1‑140 (MQ=255) gtggtgTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCaga < 1:91350/140‑1 (MQ=255) tggtgTTCAACACTGCACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCagag < 2:412296/140‑1 (MQ=255) | GCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAG > NC_000913/3875729‑3875990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |