Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,917,574	C→T	G231D (GGC→GAC)	atpG ←	ATP synthase F1 complex subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,917,574	0	C	T	100.0%	155.1 / NA	47	G231D (GGC→GAC)	atpG	ATP synthase F1 complex subunit gamma
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/24);  total (23/24)

TTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATC  >  NC_000913/3917438‑3917674                                                                                                                                         |                                                                                                     ttCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCACCACCATAAGGGGGGCGTGCTCGCTCGCCAAGTTTTCAACCACGTCCt                                                                                                   >  2:250295/1‑140 (MQ=255)         tAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCGTGCACGCAGGCCAGGTTTTCAACCACGTCCTGATAAAcc                                                                                           >  2:377173/1‑140 (MQ=255)            tGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTga                                                                                        >  2:794324/1‑140 (MQ=255)                ggCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGAtt                                                                                    <  2:729430/140‑1 (MQ=255)                 gCCTGACGAGCTTTATTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTc                                                                                   >  2:126934/1‑140 (MQ=255)                      aCGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACAt                                                                              >  2:479399/1‑140 (MQ=255)                      aCGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACAt                                                                              >  2:635552/1‑140 (MQ=255)                          gCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAacg                                                                          <  1:91101/140‑1 (MQ=255)                               gttgtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACgca                                                                     <  2:459964/140‑1 (MQ=255)                               gagacagaCCAACTGGAGCTCTTTAATCATGCTGCCGACATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACgca                                                                     <  1:398959/133‑1 (MQ=255)                                     taCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCTTGATAAAGCTGCGAGTCGACATAACGACGCAGCAggg                                                               >  1:376742/1‑140 (MQ=255)                                     taCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCGTGATAAAGCTGGGAGTCGACATAACGACGCAGCAggg                                                               >  1:229991/1‑140 (MQ=255)                                     taCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAacgacg                                                                       <  1:415199/132‑1 (MQ=255)                                     taCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAacgacg                                                                       >  2:415199/1‑132 (MQ=255)                                     taCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAggg                                                               >  1:741760/1‑140 (MQ=255)                                        cAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTAt                                                            >  1:478838/1‑140 (MQ=255)                                          aCTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATcc                                                          >  1:47867/1‑140 (MQ=255)                                          aCTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATcc                                                          >  1:785091/1‑140 (MQ=255)                                             gCAGCTCTTTAATCAGGGTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCGCCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGc                                                       <  1:126934/140‑1 (MQ=255)                                             gCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACCTCCTGATACACCCGAGGTTCGACATCACGAAGCAGCAGGGTATCCAGc                                                       >  2:239416/1‑140 (MQ=255)                                               aGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTACTGATAAAACTGCGGTTCGACATAACGACGGAGCAGGGTATCCAGCaa                                                     >  2:354735/1‑140 (MQ=255)                                                      tAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCaa                                                     >  1:334197/1‑133 (MQ=255)                                                      tAATCAGGCTGCCGCCATTGTCGGTCGCGGCTGTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCaa                                                     <  2:334197/133‑1 (MQ=255)                                                             gCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCgg                                       >  1:495466/1‑140 (MQ=255)                                                             gCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCgg                                       <  2:479210/140‑1 (MQ=255)                                                              cTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATCACGACGCCGCAGGGTAACCAGAAACGCCTTCGGATCggg                                      >  2:346676/1‑140 (MQ=255)                                                                gccgccATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGAt                                          <  1:562630/134‑1 (MQ=255)                                                                gccgccATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGAt                                          >  2:562630/1‑134 (MQ=255)                                                                 ccgccATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGAAGCAGCAGGGTAGCCGGCAACGGCTTCGGATCGGGTTc                                   >  1:213395/1‑140 (MQ=255)                                                                   gccATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGt                                 <  2:47867/140‑1 (MQ=255)                                                                    ccATTGTCGGTCGCGTCTTTCAGCGTCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTa                                <  1:346676/140‑1 (MQ=255)                                                                    ccATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTa                                <  2:79942/140‑1 (MQ=255)                                                                    ccATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTa                                <  2:134165/140‑1 (MQ=255)                                                                    ccATTGTCGGTCGCGGCGTTCATCGCCACCATACGTGCGCCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTa                                <  2:376742/140‑1 (MQ=255)                                                                         gtcggtcgCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGt                           <  1:635552/140‑1 (MQ=255)                                                                             gtcaCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATc                       >  1:474645/1‑140 (MQ=255)                                                                              tcgCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATcc                      <  2:495466/140‑1 (MQ=255)                                                                               cgcgGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAgg                            >  2:413827/1‑133 (MQ=255)                                                                               cgcgGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAgg                            <  1:413827/133‑1 (MQ=255)                                                                                gcgGCTTTCATCGCCCCCATACAGGCGGCCTGCTCGCCGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCa                    <  1:239416/140‑1 (MQ=255)                                                                                      ttCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAttt              <  2:741760/140‑1 (MQ=255)                                                                                            gCCACCATACGGGCGGCCTGCTCGCTGGCCAAGTTATCAGCCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGttt        <  2:229991/140‑1 (MQ=255)                                                                                              caccaTACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGttt        <  2:325350/138‑1 (MQ=255)                                                                                              caccaTACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGttt        >  1:325350/1‑138 (MQ=255)                                                                                                ccaTACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGa    <  2:457815/140‑1 (MQ=255)                                                                                                ccaTACGGGCGGCCTACTCGCTTGCTAGGCTTTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGa    <  1:250295/140‑1 (MQ=255)                                                                                                  aTACGGGCGGCCTGCTCGCTGGTCATGTTGTCAACCACGTCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatc  <  1:377173/140‑1 (MQ=255)                                                                                                                                         |                                                                                                     TTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATC  >  NC_000913/3917438‑3917674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑