Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	458,411	C→T	P329L (CCG→CTG)	clpX →	ATP‑dependent Clp protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	458,411	0	C	T	100.0%	180.8 / NA	52	P329L (CCG→CTG)	clpX	ATP‑dependent Clp protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/22);  total (30/22)

GCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCC  >  NC_000913/458283‑458547                                                                                                                                 |                                                                                                                                         gctgGCGCAGGTTGAACCGGAAGATCTGACCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGccc                                                                                                                               <  1:282817/140‑1 (MQ=255)    ggCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGa                                                                                                                            <  1:684848/140‑1 (MQ=255)             tGAACCGGAAGATCTAATCAAGATTCGTCTTATCCCTAAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGt                                                                                                                   <  1:239075/140‑1 (MQ=255)                    gAAGATCTGATCAAGTTTGGTCATATCCCGGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGgc                                                                                                            <  1:322619/140‑1 (MQ=255)                     aaGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGgcg                                                                                                           <  1:417808/140‑1 (MQ=255)                     aaGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATCGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGgcg                                                                                                           <  1:361386/140‑1 (MQ=255)                         tCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGt                                                                                                       <  1:573220/140‑1 (MQ=255)                              tCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAGACGCCCTGACCATGCAGACTCAGGCGCTGTTTAAt                                                                                                  >  2:361386/1‑140 (MQ=255)                              tCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGgcg                                                                                                           >  2:138697/1‑131 (MQ=255)                              tCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGgcg                                                                                                           <  1:138697/131‑1 (MQ=255)                                 aGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTg                                                                                               <  2:618611/140‑1 (MQ=255)                                    ttGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCCAGCCGTGTCAGGCTCTGTTTAATCTGGaa                                                                                            >  1:331267/1‑140 (MQ=255)                                        tCTTATCCCTGAGTTTAATGGTTGTCTGCCGTTTGACGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCg                                                                                        <  2:331267/140‑1 (MQ=255)                                            aTCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGa                                                                                             <  2:682893/131‑1 (MQ=255)                                            aTCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGa                                                                                             >  1:682893/1‑131 (MQ=255)                                            aTCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGa                                                                                    >  2:119564/1‑140 (MQ=255)                                             tCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGAt                                                                                   >  2:740106/1‑140 (MQ=255)                                             tCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAAAGCCCTGACCAAGGAGTTTCAGGCGCGGTTTAATCTGGAAGGCGTGGAt                                                                                   >  2:299576/1‑140 (MQ=255)                                              agacaggTTTATTGGTCGTCTGCCGGTTGTCTCGATGTTGAATGAACTAAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATc                                                                                  <  2:360945/134‑1 (MQ=255)                                                  gAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGAt                                                                                   >  2:528420/1‑135 (MQ=255)                                                  gAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGAt                                                                                   <  1:528420/135‑1 (MQ=255)                                                  gAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGa                                                                              <  1:553138/140‑1 (MQ=255)                                                  gAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGa                                                                              >  1:829091/1‑140 (MQ=255)                                                       tATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTcc                                                                         >  1:408876/1‑140 (MQ=255)                                                       tATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTcc                                                                         >  1:638482/1‑140 (MQ=255)                                                        aTTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCg                                                                        >  2:707243/1‑140 (MQ=255)                                                             tcgtcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACg                                                                   >  1:688405/1‑140 (MQ=255)                                                               gtcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAg                                                                 >  2:634298/1‑140 (MQ=255)                                                               gtcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGACGGGGATCTTGAATTCCGtctgtct                                                                 >  1:360945/1‑134 (MQ=255)                                                                tcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAgg                                                                >  2:811457/1‑140 (MQ=255)                                                                  tGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGgcg                                                              <  1:634298/140‑1 (MQ=255)                                                                  tGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGgcg                                                              <  2:688405/140‑1 (MQ=255)                                                                  tGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGgcg                                                              <  1:498547/140‑1 (MQ=255)                                                                           tCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCt                                                     <  1:532562/140‑1 (MQ=255)                                                                                      aatgaaCTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGaa                                          >  1:531333/1‑140 (MQ=255)                                                                                           aCTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGa                                     >  2:458555/1‑140 (MQ=255)                                                                                           aCTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGa                                     >  2:696256/1‑140 (MQ=255)                                                                                            cTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCAAAGAAAGCGAt                                    >  2:558589/1‑140 (MQ=255)                                                                                                gCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGgcg                                >  1:64136/1‑140 (MQ=255)                                                                                                 cGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGgcgc                               >  1:464808/1‑140 (MQ=255)                                                                                                 cGAAGAAGCTCTGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGgcgc                               >  1:497038/1‑140 (MQ=255)                                                                                                         ctctGATTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAAcc                       >  2:624926/1‑140 (MQ=255)                                                                                                              aTTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATg                                   >  2:733022/1‑123 (MQ=255)                                                                                                              aTTCAGATCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATg                                   <  1:733022/123‑1 (MQ=255)                                                                                                                    aTCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGCGCCCGTgg            >  1:661783/1‑140 (MQ=255)                                                                                                                     tCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGc           <  1:696256/140‑1 (MQ=255)                                                                                                                     tCCTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGc           <  1:458555/140‑1 (MQ=255)                                                                                                                      ccTCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGcc          >  2:819995/1‑140 (MQ=255)                                                                                                                        tCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTg        >  2:63519/1‑140 (MQ=255)                                                                                                                        tCAAAGAGCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTg        >  2:201590/1‑140 (MQ=255)                                                                                                                              agCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTcc  <  2:531333/140‑1 (MQ=255)                                                                                                                              agCTGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCCTTcc  >  2:786364/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                         GCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCC  >  NC_000913/458283‑458547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑