Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,443	T→C	D64G (GAT→GGT)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,443	0	T	C	100.0%	88.9 / NA	26	D64G (GAT→GGT)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/5);  total (21/5)

TACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGG  >  NC_000913/3925306‑3925578                                                                                                                                          |                                                                                                                                        tacgtacCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCAccc                                                                                                                                       >  1:37013/1‑140 (MQ=255)        cGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGCTCGATTTCGCCATCAGTCCGCGGAGTGCACCCActtctt                                                                                                                                >  2:282194/1‑140 (MQ=255)        cGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCActtctt                                                                                                                                >  1:586157/1‑140 (MQ=255)           cTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTa                                                                                                                             >  1:687855/1‑140 (MQ=255)                     gccacaCGTCCCCCTTGGGCTGGGGTAGCGCAAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTcc                                                                                                                   <  2:49842/138‑1 (MQ=255)                         cacGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGGCCGGCGAATGCACCCACTTCTCTTACCAGATGTCCCTTc                                                                                                               >  2:335260/1‑140 (MQ=255)                         cacGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTc                                                                                                               >  1:690024/1‑140 (MQ=255)                          acGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGACACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTcc                                                                                                              >  1:522706/1‑140 (MQ=255)                                        cTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATc                                                                                                >  1:20169/1‑140 (MQ=255)                                                       ccgccgGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTc                                                                                 >  1:625966/1‑140 (MQ=255)                                                                             tAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGCCCGCCGAGTGCACCCACTTTTTTTACCAGATGTCCCTCCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGatat                                                           <  1:89512/140‑1 (MQ=255)                                                                              aGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATAtt                                                          <  2:37013/140‑1 (MQ=255)                                                                                        aCTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATTGCCGGGTTGCAGATCAGCTGCCCCAAAGTGGCGATATTGTGTGTCaaa                                                >  2:247104/1‑140 (MQ=255)                                                                                            gATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCa                                            >  1:358115/1‑140 (MQ=255)                                                                                            gATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCa                                            >  2:629665/1‑140 (MQ=255)                                                                                            gATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCa                                            >  1:632754/1‑140 (MQ=255)                                                                                             atccccGCCTGATCGATCGTTTTCGCCATCAGCCCGCCGAGTGCCCCCACTTCTTTTACCAGATGTCCCTCCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCag                                           <  2:586157/137‑1 (MQ=255)                                                                                             aTACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCag                                           >  1:432529/1‑140 (MQ=255)                                                                                                 ccGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTc                                       >  1:629373/1‑140 (MQ=255)                                                                                                    ccTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGACCCCGAGAGTCGATATTGTGTGTCAAAAGCAGAGTCTGt                                    >  2:274545/1‑140 (MQ=255)                                                                                                     cTGATCGATCGTTTTCCCCATCAGCCCGCCGAGTGCCCCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGtt                                   <  2:629373/140‑1 (MQ=255)                                                                                                             tcgCTTTCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGGCCCAGAGTTTCGGTATATTGTGTCACAAGCAGAGTCTGTAGACCCATa                           >  2:399676/1‑140 (MQ=255)                                                                                                                  ttCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACgcgc                      >  2:38179/1‑140 (MQ=255)                                                                                                                  ttCGCCATCAGACCGCCGAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACgcgc                      >  2:517839/1‑140 (MQ=255)                                                                                                                                  cgAGTGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGAGTCAAAAGGAGAGTCTTTTGACCCATACGCGCCGCGGCCATCGCGGcc      >  2:310455/1‑140 (MQ=255)                                                                                                                                      tGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCgg  >  1:770255/1‑140 (MQ=255)                                                                                                                                      tGCACCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCgg  >  2:68021/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGG  >  NC_000913/3925306‑3925578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑