Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,051,328 G→A intergenic (+48/‑19) csrC → / → yihI small regulatory RNA CsrC/Der GTPase‑activating protein YihI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,3280GA96.2% 164.0 / ‑1.9 52intergenic (+48/‑19)csrC/yihIsmall regulatory RNA CsrC/Der GTPase‑activating protein YihI
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (31/19);  total (32/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

CGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTC  >  NC_000913/4051200‑4051464
                                                                                                                                |                                                                                                                                        
cggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACg                                                                                                                               <  2:584251/140‑1 (MQ=255)
cggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACg                                                                                                                               >  2:567064/1‑140 (MQ=255)
      gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGGATAAAACAGATAAAAGACGAACCAt                                                                                                                         >  1:271458/1‑140 (MQ=255)
          ggATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATg                                                                                                                     <  1:567064/140‑1 (MQ=255)
                  aGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACcatc                                                                                                             <  1:132801/140‑1 (MQ=255)
                     cccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCatcatc                                                                                                          >  1:774337/1‑140 (MQ=255)
                      ccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCt                                                                                                         >  2:447356/1‑140 (MQ=255)
                        gTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTc                                                                                                       >  2:135563/1‑140 (MQ=255)
                         ttAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCa                                                                                                      <  1:135563/140‑1 (MQ=255)
                          tAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCaa                                                                                                     >  2:745432/1‑140 (MQ=255)
                                 tATGAGTCAGGAAAAAAGGCGAAAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACg                                                                                              <  1:394307/140‑1 (MQ=255)
                                   tGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACgca                                                                                            >  1:555413/1‑140 (MQ=255)
                                        cAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCaaa                                                                                       >  2:650615/1‑140 (MQ=255)
                                           gAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTC‑CGCAGCAAAGGTc                                                                                   >  2:50621/1‑140 (MQ=255)
                                                                   tCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAaca                                                            >  2:179519/1‑140 (MQ=255)
                                                                    cGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAGAGGGCATGCAAAAACGCGTCGAAAAacac                                                           >  1:246344/1‑140 (MQ=255)
                                                                    cGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAacac                                                           >  2:457909/1‑140 (MQ=255)
                                                                    cGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAacac                                                           >  1:52630/1‑140 (MQ=255)
                                                                    cGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAacac                                                           >  1:677940/1‑140 (MQ=255)
                                                                    cGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAacac                                                           >  1:696733/1‑140 (MQ=255)
                                                                      ccTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACAcgc                                                         >  1:84185/1‑140 (MQ=255)
                                                                      ccTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGaaaaa                                                              >  2:174555/1‑135 (MQ=255)
                                                                      ccTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGaaaaa                                                              <  1:174555/135‑1 (MQ=255)
                                                                            ttCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCgaggag                                                   <  2:84185/140‑1 (MQ=255)
                                                                            ttCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCgaggag                                                   <  2:774337/140‑1 (MQ=255)
                                                                            ttCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCgaggag                                                   <  1:457909/140‑1 (MQ=255)
                                                                            ttCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCAAAAAACACGCgaggag                                                   <  2:677940/140‑1 (MQ=255)
                                                                             tCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGc                                                  >  1:810987/1‑140 (MQ=255)
                                                                             tCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGc                                                  >  2:436195/1‑140 (MQ=255)
                                                                                 tgcttgctTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGa                                              >  2:449826/1‑140 (MQ=255)
                                                                                  gcttgcttTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGAt                                             <  1:436195/140‑1 (MQ=255)
                                                                                      gcttTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAgg                                         <  1:179519/140‑1 (MQ=255)
                                                                                      gcttTCTGTTAGATTCCCCCGCCAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAgg                                         <  2:271458/140‑1 (MQ=255)
                                                                                                 gATTCCGCCGCAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATAAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGAt                              >  1:108055/1‑140 (MQ=255)
                                                                                                     ccgccgCAAATCTATACCGAATTAAACAGCTGAAAAACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCa                          <  2:214472/140‑1 (MQ=255)
                                                                                                      cgccgcAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAGCACGCGAAGAGATGGATCAGGGAGCTCGTGATCGCaa                         >  1:214472/1‑140 (MQ=255)
                                                                                                      cgccgcAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGTTGAATCAGGGAGCTCGTGATCGCaa                         >  1:299662/1‑140 (MQ=255)
                                                                                                      cgccgcAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCaa                         >  2:632900/1‑140 (MQ=255)
                                                                                                      cgccgcAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCaa                         >  2:94968/1‑140 (MQ=255)
                                                                                                      cgccgcAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCaa                         >  1:136158/1‑140 (MQ=255)
                                                                                                      cgccgcAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCaa                         >  1:141562/1‑140 (MQ=255)
                                                                                                      cgccgcAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCaa                         >  1:464319/1‑140 (MQ=255)
                                                                                                        ccgcAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGc                       >  2:819961/1‑140 (MQ=255)
                                                                                                        ccgcAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACACCACGAGCTGGAACAGGTGGCTCGTGGTCGCAAGc                       >  2:393090/1‑140 (MQ=255)
                                                                                                           cAAATCTATACTGAATAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTc                    <  1:85462/140‑1 (MQ=255)
                                                                                                                  ataCTGAATAAAACAGCAAAAACACGAACCACTATTAAAACATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGaaaaa             <  2:246344/140‑1 (MQ=255)
                                                                                                                        aaTAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGaaaaaa            <  2:556261/135‑1 (MQ=255)
                                                                                                                        aaTAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGaaaaaa            >  1:556261/1‑135 (MQ=255)
                                                                                                                        aaTAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAAcgtcg       <  2:696733/140‑1 (MQ=255)
                                                                                                                          tAAAACAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGTAGCTGGATTAGGCAGCACGTGATCGTAAGCGTCAGAAAAAACGTCGTg     >  2:319235/1‑140 (MQ=255)
                                                                                                                             aaCAGCTAAAAGACGCACCATTCTGAAACCATCATCTGCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTc  <  1:393090/140‑1 (MQ=255)
                                                                                                                             aaCAGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTc  <  2:810987/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                        
CGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACGCGAGGAGCTGGATCAGGAAGCTCGTGATCGCAAGCGTCAGAAAAAACGTCGTGGTC  >  NC_000913/4051200‑4051464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑