Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,074,623 A→G S295S (TCA→TCG)  yihV → 6‑deoxy‑6‑sulfofructose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,074,6230AG97.5% 133.4 / NA 40S295S (TCA→TCG) yihV6‑deoxy‑6‑sulfofructose kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (18/21);  minor base C (0/1);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGGCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTG  >  NC_000913/4074496‑4074750
                                                                                                                               |                                                                                                                               
cgcTGGCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATaa                                                                                                                     <  1:201278/140‑1 (MQ=255)
               gggATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATg                                                                                                      >  1:112444/1‑140 (MQ=255)
                    ttAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTGTGTCGCTTTTTGTAGAAAATGGCAGGGTGATGGtttt                                                                                                 >  1:220000/1‑140 (MQ=255)
                      aGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTc                                                                                               >  1:100682/1‑140 (MQ=255)
                             tcagtcCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAAt                                                                                        <  2:112444/140‑1 (MQ=255)
                               agtcCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAAttt                                                                                      >  1:668574/1‑140 (MQ=255)
                                              cGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCg                                                                       <  2:191223/140‑1 (MQ=255)
                                              cGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCg                                                                       >  2:696201/1‑140 (MQ=255)
                                                gtgtAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGaa                                                                     <  1:645009/140‑1 (MQ=255)
                                                  gtAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACt                                                                   <  1:696201/140‑1 (MQ=255)
                                                  gtAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACt                                                                   <  2:54737/140‑1 (MQ=255)
                                                   tAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTa                                                                  >  2:137931/1‑140 (MQ=255)
                                                           ttAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTaa                                                          >  1:13433/1‑140 (MQ=255)
                                                           ttAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTaa                                                          >  2:194463/1‑140 (MQ=255)
                                                                   cacaCGTCCCGGTGGACGAGCCGGGATCCCTGACTGCGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGc                                                  >  2:587596/1‑140 (MQ=255)
                                                                    acacGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCa                                                 <  1:101232/140‑1 (MQ=255)
                                                                    acacGTCCCGGTGGACGAGCCGGGATCCCTGACTGCGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCa                                                 <  1:587596/140‑1 (MQ=255)
                                                                      acGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACg                                               <  1:517755/140‑1 (MQ=255)
                                                                        gTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGAc                                             <  2:100682/140‑1 (MQ=255)
                                                                          cccGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCa                                           <  2:756995/140‑1 (MQ=255)
                                                                          cccGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCa                                           <  2:11364/140‑1 (MQ=255)
                                                                           ccGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAACCTTACCGAACTAATCGGTAATCCGCGGCACGACCaa                                          >  1:375383/1‑140 (MQ=255)
                                                                                 gACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAActcctc                                    <  2:13433/140‑1 (MQ=255)
                                                                                     aGCCGGGATCCCTGACTGTGATCAATCCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGc                                <  1:347120/140‑1 (MQ=255)
                                                                                             tCCCTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGct                        <  1:162791/140‑2 (MQ=255)
                                                                                                cTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCAt                                  <  2:87897/127‑1 (MQ=255)
                                                                                                cTGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCAt                                  >  1:87897/1‑127 (MQ=255)
                                                                                                 tGACTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGc                    <  1:194463/140‑1 (MQ=255)
                                                                                                  gACTGTGATCAAACCCGTTCTTTTTTGTCGCATTTCGTACAAAATGCCCGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCg                   <  2:220000/140‑1 (MQ=255)
                                                                                                   aCTGTGATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGt                  <  1:137931/140‑1 (MQ=255)
                                                                                                      gtgATCAACCCCGATCTCTTTTTTCGCTTTTTGTACAAAATGCCAGGGAGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGTggg               <  2:375383/140‑1 (MQ=255)
                                                                                                       tgATCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGTGGGt              >  2:581289/1‑140 (MQ=255)
                                                                                                         aTCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCCCGACCAAATCCTCAAGCTGATAGCTGAGCGTGGGtat            >  1:287417/1‑140 (MQ=255)
                                                                                                         aTCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGTGGGtat            >  1:822362/1‑140 (MQ=255)
                                                                                                         aTCAAACCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGGAACCCGCGGCACGACCGACTCCTCATGCTGGTCGCTGAGCGTGGGtat            >  2:225247/1‑140 (MQ=255)
                                                                                                         aTCAAACCCGATCTTATTTGTCCCTTTTTCTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGTGGGtat            <  1:243126/140‑1 (MQ=255)
                                                                                                              aCCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGTGGGTatat          <  2:206219/137‑1 (MQ=255)
                                                                                                              aCCCGATCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGTGGGTatat          >  1:206219/1‑137 (MQ=255)
                                                                                                                   aTCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGTg                 <  2:25120/125‑1 (MQ=255)
                                                                                                                   aTCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGTg                 >  1:25120/1‑125 (MQ=255)
                                                                                                                   aTCTTTTTTGTCGCTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCTGAGCGTGGGTATATGAATATtg  >  2:708876/1‑140 (MQ=255)
                                                                                                                               |                                                                                                                               
CGCTGGCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTG  >  NC_000913/4074496‑4074750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑