Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,093,113	A→G	intergenic (‑290/+11)	frvA ← / ← rhaM	putative PTS enzyme IIA component FrvA/L‑rhamnose mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,093,113	0	A	G	100.0%	259.9 / NA	74	intergenic (‑290/+11)	frvA/rhaM	putative PTS enzyme IIA component FrvA/L‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/38);  total (36/38)

ATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCA  >  NC_000913/4092977‑4093249                                                                                                                                         |                                                                                                                                          aTGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCAt                                                                                                                                        >  2:649044/1‑140 (MQ=255) aTGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCAt                                                                                                                                        <  1:649044/140‑1 (MQ=255)        aTTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAGAATATTGAGCCGGAGGCATCTGTGGCGCAtctgtctc                                                                                                                                 >  1:215838/1‑133 (MQ=255)          tAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATGGAAATTTAAAATGTTGTGCCGGAGGGATCTCAGGCGCATTGGGCAAtt                                                                                                                               >  2:394788/1‑140 (MQ=255)           aaTGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTa                                                                                                                              <  1:193811/140‑1 (MQ=255)             tGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACg                                                                                                                            >  2:771113/1‑140 (MQ=255)               ggTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGc                                                                                                                          >  2:730658/1‑140 (MQ=255)               ggTTATTCATTATTTATGTGATCTACTCACAATGTATTAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGc                                                                                                                          <  1:394788/140‑1 (MQ=255)                 ttATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAAAATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAg                                                                                                                        >  1:466784/1‑140 (MQ=255)                  tATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAgg                                                                                                                       <  2:130047/140‑1 (MQ=255)                  tATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAgg                                                                                                                       >  1:73217/1‑140 (MQ=255)                  tATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAgg                                                                                                                       >  1:673365/1‑140 (MQ=255)                  tATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAgg                                                                                                                       >  1:502559/1‑140 (MQ=255)                   aTTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACgg                                                                                                                           <  2:519135/135‑1 (MQ=255)                   aTTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACgg                                                                                                                           >  1:519135/1‑135 (MQ=255)                   aTTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGt                                                                                                                      >  1:604592/1‑140 (MQ=255)                         tattTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTaaaaaa                                                                                                                >  2:119846/1‑138 (MQ=255)                         tattTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAaca                                                                                                                >  1:732415/1‑140 (MQ=255)                         tattTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTAGGCAATTACg                                                                                                                            >  1:306496/1‑128 (MQ=255)                         tattTTTGTGATTTACTCACAATCTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACg                                                                                                                            <  2:306496/128‑1 (MQ=255)                               tgtgATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAGTCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACActtctt                                                                                                          >  1:603272/1‑140 (MQ=255)                                  gATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCa                                                                                                       <  1:29577/140‑1 (MQ=255)                                  gATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCa                                                                                                       <  1:573408/140‑1 (MQ=255)                                     ttACTCACAATGTATGAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCt                                                                                                    <  1:359891/140‑1 (MQ=255)                                     ttACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCt                                                                                                    <  1:119846/140‑1 (MQ=255)                                         tCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAGTCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCt                                                                                                <  2:603272/140‑1 (MQ=255)                                             aaTGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCTGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCCGCTCCCTACTc                                                                                            >  2:627916/1‑140 (MQ=255)                                             aaTGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGTGCATTGGGAAATTACGTCAGGGAAAAAACTAGTTTTAGCTCGCTtctc                                                                                            >  2:348327/1‑140 (MQ=255)                                                gTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCAcc                                                                                         <  2:757658/140‑1 (MQ=255)                                                 tATCAGGGAAATATAGCTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCg                                                                                        <  1:372434/140‑1 (MQ=255)                                                 tATCAGGGAAATATAACCTACCGGGAGATGTCATACGTTCAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCg                                                                                        <  2:324569/140‑1 (MQ=255)                                                      gggAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTg                                                                                   <  2:457436/140‑1 (MQ=255)                                                         aaaTATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAACTACGGCAGATAAAACAACTCTTGCAGCTCGCCACTCACCCCGCTGttt                                                                                >  2:345518/1‑139 (MQ=255)                                                          aaTATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAt                                                                               <  2:732415/140‑1 (MQ=255)                                                           atatAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTCAAAAAATCCTTTCAGCGCGCTCCTCACCCGGCTGttttt                                                                              >  2:358878/1‑137 (MQ=255)                                                           atatAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTa                                                                                               <  2:415557/123‑1 (MQ=255)                                                           atatAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTa                                                                                               >  1:415557/1‑123 (MQ=255)                                                           atatAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCATGCTGCGCGCTACTCACCGGGCTGTTAT‑c                                                                             >  1:324569/1‑140 (MQ=255)                                                           atatAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAAGTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTT‑c                                                                             >  2:522615/1‑140 (MQ=255)                                                                aCTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGt                                                                        <  2:466784/140‑1 (MQ=255)                                                                aCTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGt                                                                        <  1:430955/140‑1 (MQ=255)                                                                 cTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGtt                                                                       >  1:485912/1‑140 (MQ=255)                                                                 cTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCAGGCTGTTAT‑TCggggt                                                                       >  2:780043/1‑138 (MQ=255)                                                                  tttccGGGAGATGTAATATGTTGAACTTTGCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTa                                                                      <  1:349667/137‑1 (MQ=255)                                                                  ttACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTa                                                                      <  1:522615/140‑1 (MQ=255)                                                                   tACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCGTTGGGCAATTACGGCAGGTAAAAACCTTCTTGCAGCTCGCTACTCACCCGGCTGTTAT‑CCGGGTTAg                                                                     >  2:536399/1‑140 (MQ=255)                                                                    aCCGGGAGTTGTAATATTTTTAGTTTTTCATTTGGTAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGc                                                                    <  2:378528/140‑1 (MQ=255)                                                                    aCCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGc                                                                    <  2:604592/140‑1 (MQ=255)                                                                    aCCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTAGCAGCTCCCTAGTCGCCGGGCTGTTAT‑CCGGGTTAGc                                                                    >  1:269394/1‑140 (MQ=255)                                                                    aCCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAAAACTTATTGGCGCGCGCTACTCGCCGGGCTGTTAT‑CCGGGGTAGc                                                                    >  2:321347/1‑140 (MQ=255)                                                                     ccGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCg                                                                   <  2:485912/140‑1 (MQ=255)                                                                      cGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAGAAACTTCTTTCATCCCGCTACTCACCCGGCCTTTAT‑CCGGGTTAGCgg                                                                  >  2:386434/1‑140 (MQ=255)                                                                      cGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAAAACTTCTTGCAGCTCGCTACTCACCCGGCTGTTAT‑CCGGGGTAGCgg                                                                  >  1:674315/1‑140 (MQ=255)                                                                        ggAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGc                                                                >  1:551148/1‑140 (MQ=255)                                                                               tAATATGTTTAATTTTTCAAATGGAAATTTATAATGTTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACAt                                                         <  2:269394/140‑1 (MQ=255)                                                                               tAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACAt                                                         <  1:612585/140‑1 (MQ=255)                                                                               tAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACAt                                                         <  2:673365/140‑1 (MQ=255)                                                                                aaTATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATc                                                        <  1:780043/140‑1 (MQ=255)                                                                                 atatGTTTAATTTTTGAAATCGCAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCg                                                       <  1:358878/140‑1 (MQ=255)                                                                                  tatGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGGAAAAAACTTATTGCAACTCGCTAATCCCCCGGCAGTTATAACGGGATA‑AGGGCATAACATCgg                                                      >  2:260573/1‑140 (MQ=255)                                                                                      tttAATTTTTCAAATCGAAATTTAAGATAGTGTGCCGAAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCat                                                  <  1:348327/140‑1 (MQ=255)                                                                                       ttAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAAAACTTCTTGCAGCTCGCTACTCACCCGGCGGTTAT‑CCGGGGTAGCGGGCATAACATCGGTCata                                                 >  2:612585/1‑140 (MQ=255)                                                                                         aaTTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCatata                                               >  2:648681/1‑140 (MQ=255)                                                                                         aaTTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCACCTCACCGCGCTCTTTA‑CCGGGGTAACGGGAATAAAATCGGTCatata                                               >  2:364564/1‑140 (MQ=255)                                                                                           tttttCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATAtt                                             <  2:502559/140‑1 (MQ=255)                                                                                              ttCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTcc                                          >  1:446853/1‑140 (MQ=255)                                                                                                    tCGAAATTTAAAATATTTTGCCAGAGGCATCGCTGTCGCTTTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAAc                                    <  2:319290/140‑1 (MQ=255)                                                                                                     cGAAATTTAAAATATTGTGCCGCAGGCATTTCTGGCGTATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACg                                   <  1:364564/140‑1 (MQ=255)                                                                                                      gAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGt                                  <  2:73217/140‑1 (MQ=255)                                                                                                        aaTTTAAAATATTGTTCCGGAGTCAGCTCTGGCGCATAGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTg                                <  1:321347/140‑1 (MQ=255)                                                                                                                atatTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACAt                        <  1:648681/140‑1 (MQ=255)                                                                                                                atatTGTGCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACAt                        <  2:740198/140‑1 (MQ=255)                                                                                                                    tgtgCCGGAGGCATCTCTGGCGCATTGGGCAATTACGGCAGGTAAAACACTTCTTTCAGCTCGCTACTCCCCCGGCTGTTAT‑CCGGGGTAGCGGGCATAAAATCGGTCATATATTTCACCCAGCGTTGGCAAAAATCAgg                    >  2:232086/1‑139 (MQ=255)                                                                                                                                      ggCGCATTGGGCAATTACGGCAGGTCAAAGACTTATTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCa  <  1:260573/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          ATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTAT‑CCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCA  >  NC_000913/4092977‑4093249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑