| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,094,683 | C→T | intergenic (‑411/+40) | rhaD ← / ← rhaA | rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,094,683 | 0 | C | T | 92.3% | 32.5 / NA | 13 | intergenic (‑411/+40) | rhaD/rhaA | rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (6/6); minor base A (1/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTT > NC_000913/4094575‑4094817 | aCAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCGACAACTATGGCCTACCAGTAGGCCGGATAAAGCGCTCGCGCCGCATcc > 2:213964/1‑140 (MQ=11) gCCGGATAAGGCACTTGTGCCGTATCCGGCATTCATTGCTTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGa < 2:312383/140‑1 (MQ=14) cTTGTGCCGCATCCGGCAATCAATGCCTGATGCGATGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttctt < 1:596662/140‑1 (MQ=255) ccGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCGCTCGCACCGCATCCGGCATTGTTCACCCGCGACGACAAAAAATTTATTTCTCAt > 2:378686/1‑140 (MQ=14) ccTGATGCGACGCTGACGCGTCGTAGCAGTCCTACAATTATTGCCGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAg < 1:378686/140‑1 (MQ=25) aTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCAt > 1:123735/1‑140 (MQ=255) tGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCAtt < 2:123735/140‑1 (MQ=255) gTCGCGTCTTATCAGTCCTACAGCTATTGCCGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACctg < 1:339419/140‑1 (MQ=255) cTACAACTATTGCCGACCTGTAAGCCGGACAAGGCGCACGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGt < 1:213964/140‑1 (MQ=39) cAACTATTGCCGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATCCGCGACATGCTGGCGTATCGTGACGTTgg > 1:299595/1‑140 (MQ=38) attGCCGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATa > 1:164953/2‑140 (MQ=255) gCCGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGGCTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACAt > 2:427743/1‑140 (MQ=255) cGACCTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACAttt > 2:739325/1‑140 (MQ=255) | ACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTT > NC_000913/4094575‑4094817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |