Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,137,062	T→C	H199R (CAT→CGT)	yijF ←	conserved protein YijF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,137,062	0	T	C	100.0%	152.3 / NA	44	H199R (CAT→CGT)	yijF	conserved protein YijF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/25);  total (19/25)

CGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTT  >  NC_000913/4136935‑4137189                                                                                                                                |                                                                                                                                cGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATcc                                                                                                                     >  1:624451/1‑140 (MQ=255)      ttgtgctgattgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAg                                                                                                                <  1:461420/140‑1 (MQ=255)           ctgattgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATcc                                                                                                                     >  2:205954/1‑130 (MQ=255)           ctgattgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATcc                                                                                                                     <  1:205954/130‑1 (MQ=255)            tgattgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAAc                                                                                                          <  1:188553/140‑1 (MQ=255)              attgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAg                                                                                                        >  2:684097/1‑140 (MQ=255)              attgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAg                                                                                                        >  1:552760/1‑140 (MQ=255)              attgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAg                                                                                                        >  1:61403/1‑140 (MQ=255)                tgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGca                                                                                                      >  2:126736/1‑140 (MQ=255)                tgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGca                                                                                                      >  2:683902/1‑140 (MQ=255)                            ggCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCt                                                                                          >  1:191147/1‑140 (MQ=255)                             gcgcTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTaaa                                                                                                           <  1:648330/122‑1 (MQ=255)                             gcgcTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTaaa                                                                                                           >  2:648330/1‑122 (MQ=255)                                       gCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTgcg                                                                               >  1:59666/1‑140 (MQ=255)                                       gCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTgcg                                                                               >  2:702681/1‑140 (MQ=255)                                        ccGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTgcgc                                                                              <  2:651846/140‑1 (MQ=255)                                        ccGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTgcgc                                                                              <  2:535262/140‑1 (MQ=255)                                        ccGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTgcgc                                                                              <  2:691531/140‑1 (MQ=255)                                          gTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCa                                                                            <  1:684097/140‑1 (MQ=255)                                             aGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTgcg                                                                               <  2:677418/134‑1 (MQ=255)                                             aGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTgcg                                                                               >  1:677418/1‑134 (MQ=255)                                             aGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATg                                                                         <  1:683902/140‑1 (MQ=255)                                                        gCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCAc                                                              <  2:602917/140‑1 (MQ=255)                                                           gTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAg                                                           <  2:624451/140‑1 (MQ=255)                                                                   aaaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACAGCTTCTTCCGGCGAGCCAGCGCCAATTTTATATATCACCAGCGGCGTAc                                                   >  2:240846/1‑140 (MQ=255)                                                                          aTCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTcgcg                                            >  1:161261/1‑140 (MQ=255)                                                                          aTCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTcgcg                                            >  2:205452/1‑140 (MQ=255)                                                                                  tttttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGc                                    <  1:205452/140‑1 (MQ=255)                                                                                   ttttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGcc                                   >  1:442792/1‑140 (MQ=255)                                                                                           aTGTGGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGaaa                           <  2:552760/140‑1 (MQ=255)                                                                                            tgtgGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAac                          <  1:600199/140‑1 (MQ=255)                                                                                            tgtgGGGCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAac                          <  1:62200/140‑1 (MQ=255)                                                                                                gggCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAaccacc                      <  2:59666/140‑1 (MQ=255)                                                                                                 ggCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCAccc                     <  2:161261/140‑1 (MQ=255)                                                                                                 ggCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCAccc                     <  2:61403/140‑1 (MQ=255)                                                                                                 ggCGATCTCTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCAccc                     <  2:442792/140‑1 (MQ=255)                                                                                                      tctcTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAaccac                       <  2:459597/133‑1 (MQ=255)                                                                                                      tctcTTATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAaccac                       >  1:459597/1‑133 (MQ=255)                                                                                                           tATTTAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGc           <  1:781410/140‑1 (MQ=255)                                                                                                             tttAACAAAATAACGATAACGCTCCCCCACCCGCCAGCTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCa         <  1:240846/140‑1 (MQ=255)                                                                                                             tttAACAAAATAACGATAACGCCGCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCa         <  1:126736/140‑1 (MQ=255)                                                                                                             tttAACAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCa         <  1:702681/140‑1 (MQ=255)                                                                                                                  cAAAATAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGGGAAGCCAGCTGGAAACACCCCGATATGTGCCAGCCCg    >  1:401829/1‑140 (MQ=255)                                                                                                                    aaaTAACGATAACGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAGCCACCCCAATATGTGCCAGCCCGtt  >  1:270900/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                CGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTT  >  NC_000913/4136935‑4137189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑