| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,180,277 | T→C | R94R (CGT→CGC) | rplJ → | 50S ribosomal subunit protein L10 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,180,277 | 0 | T | C | 97.1% | 111.1 / NA | 36 | R94R (CGT→CGC) | rplJ | 50S ribosomal subunit protein L10 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (19/15); minor base G (1/0); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGTTCGTAACACCCTGCTGCGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGC > NC_000913/4180155‑4180409 | gttgttCGTAACACCCTGCTGCGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTcgc > 1:772868/1‑140 (MQ=255) tAACACCCTGCTGCGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCaaa < 2:572619/124‑1 (MQ=255) tAACACCCTGCTGCGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCaaa > 1:572619/1‑124 (MQ=255) ccTGCTGCGCAGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACTCGGGCGCTGCTGATCGGCTGTTCAACGAGTACGCGAAAGCGAATGCaa > 2:308305/1‑140 (MQ=255) tgctgcGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTGGCCAGTTCAAAGAGTTCTCGAAAGCGAATGCaaaa > 2:313692/1‑140 (MQ=255) ctgcGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGGTCGACTGTTCAAAGAGTTCGCAAAAGCGAATGCAAAAtt > 2:273432/1‑140 (MQ=255) ctgcGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAAtt > 2:98946/1‑140 (MQ=255) ctgcGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAAtt > 1:776715/1‑140 (MQ=255) tgcGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAAttt < 1:98946/140‑1 (MQ=255) ccGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAgg > 2:719329/1‑140 (MQ=255) ccGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACA‑CCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGt > 1:706337/1‑140 (MQ=255) tGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCa > 2:670573/1‑140 (MQ=255) tGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCg < 1:493504/140‑1 (MQ=255) tGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCg < 2:772868/140‑1 (MQ=255) aaGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCt < 2:104864/140‑1 (MQ=255) aCTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAGAGAGTTCGAGAAGGCGGATGCAAAAGTTGACGTCAAAGCCGCTGCCtt > 2:214353/1‑140 (MQ=255) gTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAg < 2:776715/140‑1 (MQ=255) tGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATc > 2:636761/1‑140 (MQ=255) aaGACGCGTTTGTTGCTCCGACGCTCATTACATACTCCATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCg < 1:214353/140‑1 (MQ=255) aaGACGCGTTTGTTGCTCCGACCCTAATTCCATACTCGATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCg < 1:313692/140‑1 (MQ=255) gACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGc < 1:670573/140‑1 (MQ=255) ccGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCg < 1:719329/140‑1 (MQ=255) ccGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCg > 1:668449/1‑140 (MQ=255) gCATACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTC‑AAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTg < 1:461486/140‑1 (MQ=255) cATACTCTATGGAACGCCCGGGCGCCGCTGCTCGCCTCTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTg < 1:273432/140‑1 (MQ=255) aTACTCTATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGc > 1:81021/1‑140 (MQ=255) ctctATGGAACA‑CCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGAc < 2:706337/140‑1 (MQ=255) ctATGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCACAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGAcc > 2:763245/1‑140 (MQ=255) tGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAACATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGGGTCTCAGTTCGGCCACCTGGCCACTCTGCCGACCTAc > 1:247761/1‑140 (MQ=255) tGGAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTAc > 1:631317/1‑140 (MQ=255) ggAACACCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACg > 2:468580/1‑140 (MQ=255) acCCGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACgaagaa > 2:637448/1‑140 (MQ=255) ccGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGc > 1:195683/1‑140 (MQ=255) ccGGGCGCTGCTGCTCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCAGGCGTCTCAGATTGACCGCCTGGCACCTCTACCGACCTACGAAGAAGc > 2:303188/1‑140 (MQ=255) gctgctgctCGCCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGc < 2:100978/140‑1 (MQ=255) ctgctCGCCTGTTCAAAGAGTTCGCGAAATCGAATGCCAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGc < 1:308305/140‑1 (MQ=255) | GTTGTTCGTAACACCCTGCTGCGCCGTGCTGTTGAAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGC > NC_000913/4180155‑4180409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |