Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,201,888	T→C	intergenic (‑200/‑38)	zraP ← / → zraS	zinc responsive, periplasmic protein with chaperone activity/sensory histidine kinase ZraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,201,888	0	T	C	100.0%	132.8 / NA	39	intergenic (‑200/‑38)	zraP/zraS	zinc responsive, periplasmic protein with chaperone activity/sensory histidine kinase ZraS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/15);  total (24/15)

GGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTA  >  NC_000913/4201762‑4202021                                                                                                                               |                                                                                                                                      ggTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGaa                                                                                                                          >  1:610844/1‑140 (MQ=255) ggTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGAAGGCATGATCTCTGCTGTCAGaa                                                                                                                          >  1:374333/1‑140 (MQ=255)   tAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGGGCTGATAGAAGATGGCATGATCTCTGCTGTCAGAAAg                                                                                                                        >  1:363878/1‑140 (MQ=255)   tAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAg                                                                                                                        >  1:95588/1‑140 (MQ=255)   tAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAg                                                                                                                        >  1:516026/1‑140 (MQ=255)              gATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAgg                                                                                                             <  2:95588/140‑1 (MQ=255)                    cGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGCCATTTTTACTCATTGAAACTTAAGCCTTTGGGATACAGCGCAGGGAAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAaaga                                                                                                       >  1:716907/1‑140 (MQ=255)                         aaaaaTGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAaagaagaag                                                                                                  >  2:411906/1‑140 (MQ=255)                         aaaaaTGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAaagaagaag                                                                                                  >  2:146930/1‑140 (MQ=255)                          aaaaTGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAagaagaaga                                                                                                 >  2:573270/1‑140 (MQ=255)                           aaaTGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGAt                                                                                                <  2:610844/140‑1 (MQ=255)                                       tgctgcGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCa                                                                                    <  1:411906/140‑1 (MQ=255)                                       tgctgcGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCa                                                                                    <  2:693551/140‑1 (MQ=255)                                       tgctgcGGGTAGCGAGTCATTTATACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCa                                                                                    <  1:263934/140‑1 (MQ=255)                                       tgctgcGGGTAGCGAGACATTTTTACTCATTGATACTTAGGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCa                                                                                    <  2:385507/140‑1 (MQ=255)                                              ggTAGCGAGTCATTTTTTCTCACTGAAACTTCAGCCTTTATGTTACAGGGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGTAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCt                                                                             <  2:299018/140‑1 (MQ=255)                                              ggTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCt                                                                             >  1:820915/1‑140 (MQ=255)                                                   cGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGATCTAAAGa                                                                        >  2:643885/1‑140 (MQ=255)                                                      ggcATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCCGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTc                                                                     >  2:657313/3‑140 (MQ=255)                                                      gTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTc                                                                     <  1:146930/140‑1 (MQ=255)                                                      gTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGATCTAAAGACTc                                                                     <  1:643885/140‑1 (MQ=255)                                                        cATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCt                                                                   <  1:657313/140‑1 (MQ=255)                                                                   aTTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAATAAGAAGATCCGTATTATGCAACGTTTTAAATACTCCTTAGCTAAATgg                                                        >  2:315042/1‑140 (MQ=255)                                                                     tGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGtt                                                      >  2:666730/1‑140 (MQ=255)                                                                     tGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGtt                                                      >  1:693288/1‑140 (MQ=255)                                                                             aaGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGGGTTTTATGGAACGTTCGAAAGACTCCTTAGCTAAATGGTTAAGCGCGa                                              >  2:290819/1‑140 (MQ=255)                                                                             aaGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAGAGAGTCATTAGCTAAATGGTTAAGCGCGa                                              >  1:243270/1‑140 (MQ=255)                                                                               gCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGAtc                                            >  2:743469/1‑140 (MQ=255)                                                                               gCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACATTCTAAACACTCCTCAGCTAATTGGTTAAGCGCGAtc                                            >  1:360541/1‑140 (MQ=255)                                                                                    tgtgTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTccc                                       >  1:14423/1‑140 (MQ=255)                                                                                          aCAGCGCAGGGTAAGCGTTGATTAAAGATGGTATGTTCTCTGCTGTCATAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGt                                 <  2:243270/140‑1 (MQ=255)                                                                                          aCAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGt                                 >  2:505521/1‑140 (MQ=255)                                                                                              cgcAGGGTAAGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCGTTACCTAAATGGTTAAACGCGTTCCTCCCCGTGGTCAtt                             >  2:309049/1‑140 (MQ=255)                                                                                                       aGCGCTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCtg                    >  2:649171/1‑140 (MQ=255)                                                                                                        gcgcTGATAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCtgg                   <  2:516026/140‑1 (MQ=255)                                                                                                        gcgcTGATAAAAGATGCAATGATCTCTGCTGGCAGTAAGCGATGAGCAAGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCtgg                   <  2:363878/140‑1 (MQ=255)                                                                                                              aTAAAAGATGGCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGAt             >  1:171187/1‑140 (MQ=255)                                                                                                                        gCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGt   >  1:466635/1‑140 (MQ=255)                                                                                                                        gCATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGt   <  2:693288/140‑1 (MQ=255)                                                                                                                         cATGATCTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTa  <  1:666730/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                      GGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTA  >  NC_000913/4201762‑4202021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑