Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,271,224	T→C	N883S (AAC→AGC)	uvrA ←	excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,271,224	0	T	C	100.0%	82.5 / NA	24	N883S (AAC→AGC)	uvrA	excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

CGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCA  >  NC_000913/4271090‑4271359                                                                                                                                       |                                                                                                                                        cGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCAGTTTTGATGACGACGAGATTGTGCTGAATCGCCACAATGGTGCTGccc                                                                                                                                    >  2:223567/1‑140 (MQ=255)        cgcgACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGCGATAGCGCTCAATCACAACAAAGGTGCTGCCCTGATcct                                                                                                                             >  2:370154/1‑138 (MQ=255)               gTTTCTGGCGTACCGTAGACGACGATCTCACCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGttt                                                                                                                      <  1:223567/140‑1 (MQ=255)                  tCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATg                                                                                                                   >  2:537542/1‑140 (MQ=255)                          aCCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGt                                                                                                           >  1:345988/1‑140 (MQ=255)                            cGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCg                                                                                                         <  1:418257/140‑1 (MQ=255)                              gagaCGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAg                                                                                                       <  2:345988/140‑1 (MQ=255)                                 aCGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAg                                                                                                    <  1:77809/140‑1 (MQ=255)                                            gccgccACCACTGCCACCTTCTAGTTCCATGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAatat                                                                                         <  1:250967/140‑1 (MQ=255)                                                                       cAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCt                                                              <  1:80191/140‑1 (MQ=255)                                                                          gTCGACAATCCAGTCAGTGGTTATGATCACGTCGAGATTGTGCTCAATTACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCtcgt                                                           <  2:343728/140‑1 (MQ=255)                                                                          gTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCtcgt                                                           <  1:537542/140‑1 (MQ=255)                                                                          gTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCtcgt                                                           <  2:128913/140‑1 (MQ=255)                                                                             gACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCga                                                        >  1:638197/1‑140 (MQ=255)                                                                              aCAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCgag                                                       >  2:194026/1‑140 (MQ=255)                                                                                         aGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCg                                            >  2:82584/1‑140 (MQ=255)                                                                                            ggTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGGAGACCGGCGGTGGGGTCGTCGAGAATATACAGCGTCt                                         >  1:211409/1‑140 (MQ=255)                                                                                            ggTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCt                                         >  1:619226/1‑140 (MQ=255)                                                                                                                  tgCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACa                    <  1:490645/139‑1 (MQ=255)                                                                                                                  tgCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACa                    >  2:490645/1‑139 (MQ=255)                                                                                                                  tgCTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGACGGCTCGTCGAGAATAGACAGCGTCGGCCCAGTGCCGCGTTTTGACAg                   >  2:294975/1‑140 (MQ=255)                                                                                                                    cTCAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGtt                 >  1:31036/1‑140 (MQ=255)                                                                                                                      cAATCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCa               <  1:82584/140‑1 (MQ=255)                                                                                                                       aaTCACCACAATGGTGCTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCAc              >  1:146094/1‑140 (MQ=255)                                                                                                                                   ggTGCTGCCCTGATCGCGCAGTATACGCACTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCa  <  1:346696/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCA  >  NC_000913/4271090‑4271359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑