Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,303,598	A→G	V171A (GTA→GCA)	mdtN ←	putative multidrug efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,303,598	0	A	G	100.0%	142.5 / NA	41	V171A (GTA→GCA)	mdtN	putative multidrug efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/21);  total (20/21)

CATCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCA  >  NC_000913/4303461‑4303729                                                                                                                                          |                                                                                                                                    cATCAAACGGCGCGCGAACGGTCGCCATTTCCAGAGGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCg                                                                                                                                   <  2:340849/140‑1 (MQ=255)  aTCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTAACGGCGCGGGCGGCGGACTGCGCCTGTAACAATGCgg                                                                                                                                  >  1:227958/1‑140 (MQ=255)          gcgcgcGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGa                                                                                                                          >  2:25116/1‑140 (MQ=255)            gcgcgAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATc                                                                                                                        <  1:141410/140‑1 (MQ=255)             cgcgAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATcc                                                                                                                       >  2:21769/1‑140 (MQ=255)                                    tGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGt                                                                                                >  2:134402/1‑140 (MQ=255)                                         tttGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTg                                                                                           >  2:492016/1‑140 (MQ=255)                                         tttGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTg                                                                                           >  1:745563/1‑140 (MQ=255)                                           tGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCa                                                                                         <  1:21769/140‑1 (MQ=255)                                           tGGTCAGGGCAATATCCGCTTCGACCACCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCa                                                                                         <  2:712067/140‑1 (MQ=255)                                              tCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAAATCCGCTCCTGGGGGGCGCTGTGCCGTTCTTGCACgg                                                                                      >  1:297567/1‑140 (MQ=255)                                                       tatCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCt                                                                             >  1:694494/1‑140 (MQ=255)                                                            gCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGc                                                                        >  1:62586/1‑140 (MQ=255)                                                            gCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGc                                                                        >  1:474460/1‑140 (MQ=255)                                                            gCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATACGCTTCTGAGGCGCGCTGCGCCGTTGTTGCACGGTCAACATCTTCCGc                                                                        >  2:333574/1‑140 (MQ=255)                                                                    cgccgcACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACaa                                                                <  1:97469/140‑1 (MQ=255)                                                                         cACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACct                                                           <  2:62586/140‑1 (MQ=255)                                                                          aCGCTGGGCAACTAATGTATCCTCGCCGCTGACGGCGCTTGCGGCTGATTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTCCTTGCACGGTCAACATCTTCCGCTGAGACAAAACctt                                                          <  2:227958/140‑1 (MQ=255)                                                                              tGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCttt                                                      <  2:745563/140‑1 (MQ=255)                                                                                      tAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACcttct                                                        >  1:777862/1‑130 (MQ=255)                                                                                      tAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACcttct                                                        <  2:777862/130‑1 (MQ=255)                                                                                             tCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGt                                       >  1:439509/1‑140 (MQ=255)                                                                                             tCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACCGTCAACATATTCTGCTGAGACAAAACCTTCATTCAGTAATGGCTCggg                                       >  2:357983/1‑139 (MQ=255)                                                                                             tCCACGCCGCTGACGCCGCTGGTGGTTGAATGCGCCTTTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGt                                       <  1:272405/140‑1 (MQ=255)                                                                                             tCCACGCCGCTGACGCCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGt                                       <  1:289049/140‑1 (MQ=255)                                                                                               cACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgc                                     >  2:525386/1‑140 (MQ=255)                                                                                               cACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCATCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgc                                     <  2:474460/140‑1 (MQ=255)                                                                                                aCGCCGCTGACGGCGGTGGCGGATGGCTGTGCCTGTAGCAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgcg                                    <  1:333574/140‑1 (MQ=255)                                                                                                aCGCCGCTGACGGCGCTTGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgcg                                    <  2:244178/140‑1 (MQ=255)                                                                                                aCGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTTAACATCTTCCGCGGAGGCAAAACCTACTTTCAGTAATGGCTCGGTgcg                                    >  2:289049/1‑140 (MQ=255)                                                                                                aCGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgcg                                    <  2:798704/140‑1 (MQ=255)                                                                                                aCGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgcg                                    >  2:57929/1‑140 (MQ=255)                                                                                                 cgccgcTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgcgg                                   <  1:492016/140‑1 (MQ=255)                                                                                                  gccgcTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgcggc                                  >  1:645410/1‑140 (MQ=255)                                                                                                   ccgcTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgcggcg                                 <  2:439509/140‑1 (MQ=255)                                                                                                   ccgcTGACGGCGCTGGCAGCTGTCTGCGCCTTTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgcggcg                                 <  2:297567/140‑1 (MQ=255)                                                                                                     gcTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACAACTTCCGCTGAGATAAAACCTTCTTTGAGTAATGGCTCGGTGCGGCGTa                               >  2:272405/1‑140 (MQ=255)                                                                                                        gACGGCGCTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATg                            <  1:17921/140‑1 (MQ=255)                                                                                                             cgcTGGCGGCTGACTGCGCCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCt                       <  1:25116/140‑1 (MQ=255)                                                                                                                            gcgcCTGTAACAATGCGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGTGACAAAACCTTCTTTCAGTAATGACTCGGTGCGGCCTATTGTATCAGTGGCCTGTTTcgcg        >  1:280766/1‑140 (MQ=255)                                                                                                                                  gTAACAATGCGGCATTAAGATCTGCCTCTCCGGCGCGTTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCa  <  1:335768/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    CATCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCA  >  NC_000913/4303461‑4303729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑