Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,310,361	T→C	E207G (GAA→GGA)	alsA ←	D‑allose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,310,361	0	T	C	100.0%	145.7 / NA	43	E207G (GAA→GGA)	alsA	D‑allose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/31);  total (12/31)

TCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACT  >  NC_000913/4310224‑4310486                                                                                                                                          |                                                                                                                              tCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTccc                                                                                                                             >  1:561348/1‑140 (MQ=255)   gcgTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGc                                                                                                                           <  2:86327/140‑1 (MQ=255)      ttAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCaa                                                                                                                        >  2:407844/1‑140 (MQ=255)         aaaCGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACtt                                                                                                                     <  2:164327/140‑1 (MQ=255)            cGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATg                                                                                                                  <  2:544556/140‑1 (MQ=255)             ggTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGc                                                                                                                 <  1:104858/140‑1 (MQ=255)             ggTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGc                                                                                                                 <  2:664565/140‑1 (MQ=255)             ggTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGc                                                                                                                 <  2:741792/140‑1 (MQ=255)              gTTTTGCAGTTCGCGGCCTACCGTCAGACGGTCGATATCTTCATTTGAAACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCg                                                                                                                <  2:248748/140‑1 (MQ=255)              gTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCg                                                                                                                <  1:58841/140‑1 (MQ=255)              gTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCg                                                                                                                <  1:407844/140‑1 (MQ=255)              gTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCg                                                                                                                <  2:22732/140‑1 (MQ=255)               ttttGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATGGCGATCGCAAATACGGGGAATCCCCGCCAACTTATGCga                                                                                                               >  2:333937/1‑140 (MQ=255)                         cgcgGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATAAGGCTAATTCCCGACAACCTATGCGAGCTATAGACg                                                                                                     >  2:382204/1‑140 (MQ=255)                          gcgGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGa                                                                                                    <  2:701408/140‑1 (MQ=255)                          gcgGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGa                                                                                                    <  2:87198/140‑1 (MQ=255)                                tACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCg                                                                                              <  1:715979/140‑1 (MQ=255)                                tACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCg                                                                                              <  1:204862/140‑1 (MQ=255)                                   cATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTAc                                                                                           <  1:547556/140‑1 (MQ=255)                                   cATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTAc                                                                                           <  2:407853/140‑1 (MQ=255)                                   cATCAGACGGACGATATCGTCAGTTGACACAGCGCTTACTATGCCGCTTCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTAc                                                                                           <  2:295439/140‑1 (MQ=255)                                      cAGACGGACGATATCGTCATTTCACGCATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCct                                                                                        <  1:382204/140‑1 (MQ=255)                                        gacggacgATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCctct                                                                                      >  1:770634/1‑140 (MQ=255)                                          cggacgATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCttt                                                                                    >  2:688921/1‑140 (MQ=255)                                             acgATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACg                                                                                 >  2:92442/1‑140 (MQ=255)                                                atatCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCaa                                                                              <  1:25441/140‑1 (MQ=255)                                                          ttGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATg                                                                    <  1:605800/140‑1 (MQ=255)                                                            gACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGAt                                                                  <  1:92442/140‑1 (MQ=255)                                                                 aTCGCTTCCTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGaa                                                             <  2:679566/140‑1 (MQ=255)                                                                 aTCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGaa                                                             >  2:826554/1‑140 (MQ=255)                                                                      ttACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGa                                                        <  2:4883/140‑1 (MQ=255)                                                                              ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCAc                                                <  2:437708/140‑1 (MQ=255)                                                                              ccGCTGCAAACGCTGCTACCGTATTTCATCAACCTGTAGCGGTCGCAACTACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCAc                                                <  2:369880/140‑1 (MQ=255)                                                                               cGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCCTTAGGCACCTGATTCATGATCAGAAACAGAAAGTCCAcc                                               >  2:370063/1‑140 (MQ=255)                                                                                  tGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAAATGATTCATGACCAGAAACAGATAGTCCACctct                                            >  2:366182/1‑140 (MQ=255)                                                                                       aCGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTAtt                                       <  1:59027/140‑1 (MQ=255)                                                                                            gctgcCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGgtga                                  >  2:98275/1‑140 (MQ=255)                                                                                                     tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGaggagg                         <  1:688921/140‑1 (MQ=255)                                                                                                         atcaCCGTATAGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTggg                     <  1:98275/140‑1 (MQ=255)                                                                                                                tataGCGGTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATcc              <  1:826554/140‑1 (MQ=255)                                                                                                                       gTCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCatgatga       >  1:97317/1‑140 (MQ=255)                                                                                                                        tCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATGGTCCACCTTTTTATTGGTGAGTGAGCAGGTGGGTTCATCCatgatgat      >  2:281742/1‑140 (MQ=255)                                                                                                                        tCGCAAATACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCatgatgat      >  2:449353/1‑140 (MQ=255)                                                                                                                        tCGCAAATACGGCGAATCCCCGCCAACTTATCCGACATACAGACGATGACCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCatgatgat      <  2:229551/140‑1 (MQ=255)                                                                                                                            aaaTACGGCGAATTCCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACt  <  2:770634/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                              TCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACT  >  NC_000913/4310224‑4310486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑