Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,365,262	A→G	L32P (CTG→CCG)	cutA ←	copper binding protein CutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,365,262	0	A	G	100.0%	191.3 / NA	54	L32P (CTG→CCG)	cutA	copper binding protein CutA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/27);  total (27/27)

CCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGT  >  NC_000913/4365134‑4365396                                                                                                                                 |                                                                                                                                       ccTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTggcg                                                                                                                             >  1:765265/1‑140 (MQ=255) ccTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTggcg                                                                                                                             >  1:810886/1‑140 (MQ=255) ccTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTggcg                                                                                                                             >  2:551621/1‑140 (MQ=255) ccTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTggcg                                                                                                                             >  1:37647/1‑140 (MQ=255) ccTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAAGTCACGCGGGCCGCCACTTTTTCCGCCGGCACTTTggcg                                                                                                                             >  2:326390/1‑140 (MQ=255)    gctgGTGAGATACGGCAGTTTTGAAAATCCTCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCt                                                                                                                          <  1:393935/140‑1 (MQ=255)         tGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATc                                                                                                                     >  2:451727/1‑140 (MQ=255)                 ggTAGTTTTTAAAATCAGCTGCGCTTCGTATGCTTGCTCCAGCTTACCCTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTg                                                                                                             <  2:346028/140‑1 (MQ=255)                      tttttAAAATCATCTTCACTTCGTATTCTGGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCt                                                                                                        <  1:326390/140‑1 (MQ=255)                      tttttAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCt                                                                                                        <  2:133529/140‑1 (MQ=255)                           aaaaTCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATc                                                                                                   <  2:680937/140‑1 (MQ=255)                                catcTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTg                                                                                              <  1:416646/140‑1 (MQ=255)                                  tcTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGcc                                                                                            <  2:551802/140‑1 (MQ=255)                                  ccTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGcc                                                                                            <  2:423186/139‑1 (MQ=255)                                        cTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACAt                                                                                      <  1:551621/140‑1 (MQ=255)                                           cGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTg                                                                                                 <  2:54191/126‑1 (MQ=255)                                           cGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTg                                                                                                 >  1:54191/1‑126 (MQ=255)                                           cGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTg                                                                                              <  1:751062/129‑1 (MQ=255)                                           cGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTg                                                                                              >  2:751062/1‑129 (MQ=255)                                           cGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGc                                                                                   >  2:573541/1‑140 (MQ=255)                                              aTTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATACTGGGCTGTCGGTTCATCTGGAGCCGTACATAGcacc                                                                                >  1:318555/1‑140 (MQ=255)                                                         aGCTTACCTTCCCAGTAATAGATAGAGGTAGGGCCCGGGATCAAGGTCCCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGAcgc                                                                     <  2:318555/140‑1 (MQ=255)                                                          gCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGAcgcg                                                                    >  1:809237/1‑140 (MQ=255)                                                          gCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGAcgcg                                                                    >  1:754357/1‑140 (MQ=255)                                                          gCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGAcgcg                                                                    >  1:28174/1‑140 (MQ=255)                                                                  tCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTCAATCCTAGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGCATTCGa                                                            >  2:143920/1‑140 (MQ=255)                                                                             gagagagGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTtcat                                                 >  2:1432/1‑140 (MQ=255)                                                                             gagagagGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTtcat                                                 <  2:810886/140‑1 (MQ=255)                                                                              agagagGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTtcatc                                                >  1:791266/1‑140 (MQ=255)                                                                               gagagGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTtcatca                                               <  2:809237/140‑1 (MQ=255)                                                                                agagGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTtcatcag                                              >  2:508329/1‑139 (MQ=255)                                                                                 gagGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAg                                             >  2:155822/1‑140 (MQ=255)                                                                                  agGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTtcat                                                 <  2:154045/135‑1 (MQ=255)                                                                                  agGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTtcat                                                 >  1:154045/1‑135 (MQ=255)                                                                                  agGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGc                                            >  1:137868/1‑140 (MQ=255)                                                                                  agGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGc                                            >  2:718951/1‑140 (MQ=255)                                                                                    gTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGGATTCGAACTTTTTTCATCAAGCAt                                          >  2:688175/1‑140 (MQ=255)                                                                                    gTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGAACATAGCAGCACAACAGACGCGGTAATCGAACTTTTTTCATCAAGCAt                                          >  2:263297/1‑140 (MQ=255)                                                                                       gcgcCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACCTAGCACCAAGACCGACACGGTATTCCAACTTGTTTCATCAAGCATaaa                                       >  2:365809/1‑140 (MQ=255)                                                                                            ggggATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCt                                  <  1:718951/140‑1 (MQ=255)                                                                                               gATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCCCGACAGACACGGCATTAGAACTTTTTTCATCAAGCATAAACACCTCGc                               >  2:258814/1‑140 (MQ=255)                                                                                               gATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGGATTGGAACTTTTTTCATGAAGCATAAACACCTCGc                               >  2:306276/1‑140 (MQ=255)                                                                                                       tCGCGCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATC‑AGCATAAACACCTCGCAAGAACAGa                      <  1:508329/140‑1 (MQ=255)                                                                                                        cgcgCAGGCCGCCAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGa                      >  1:576745/1‑140 (MQ=255)                                                                                                                ccgccAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCg              <  2:114918/140‑1 (MQ=255)                                                                                                                ccgccAGTTTTTCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCg              <  1:459012/140‑1 (MQ=255)                                                                                                                         tttCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAagagaga     <  2:754357/140‑1 (MQ=255)                                                                                                                         tttCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAagagaga     <  2:765265/140‑1 (MQ=255)                                                                                                                         tttCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAagagaga     <  1:1432/140‑1 (MQ=255)                                                                                                                         tttCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAagagaga     <  2:28174/140‑1 (MQ=255)                                                                                                                         tttCCGCCGGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAagagaga     <  1:451727/140‑1 (MQ=255)                                                                                                                         tttCCGCCGGCACTTTGGCGGCCAATTCCTGGGCTGTAGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAagagaga     <  1:258814/140‑1 (MQ=255)                                                                                                                         tttCCGCCGGCACTTTGGCGGCCAAATCCTGGGCTGTAGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAagagaga     <  1:306276/140‑1 (MQ=255)                                                                                                                         tttCCGCCGGCACTTCGGCGGCAAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAagagaga     <  1:263297/140‑1 (MQ=255)                                                                                                                            ccgccgGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGt  <  2:137868/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       CCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATCCTGGGCTGTCGCTTCATCTGGTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGT  >  NC_000913/4365134‑4365396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑