| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,381,782 | G→A | G179G (GGC→GGT) | frdA ← | fumarate reductase flavoprotein subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,381,782 | 0 | G | A | 97.3% | 116.5 / NA | 37 | G179G (GGC→GGT) | frdA | fumarate reductase flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/22); minor base C (1/0); total (15/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGG > NC_000913/4381653‑4381917 | aaCGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCcatca > 1:451092/1‑140 (MQ=255) tGGTTTAGCGCCATCCACATCCCGTCACCGGCAGCCATGCCGCCGTTGTTGTTGTAACGAAAAA‑CGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCAt < 2:352082/140‑1 (MQ=255) aGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTAc > 2:131320/1‑140 (MQ=255) atacccataccGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTCCCTACCTTCATGTCCATTGCTACCAGGcc > 1:264339/1‑140 (MQ=255) tacccataccGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTGATTGCTACCAGGCcg > 1:323309/1‑140 (MQ=255) accccGTCCCCGGAAACGAGGCCGCCTTGGGTGTTGAAACGAAAAA‑CGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAAc < 2:455406/137‑1 (MQ=255) aCCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACcat > 2:825920/1‑140 (MQ=255) aCCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACcat > 1:767912/1‑140 (MQ=255) gTAACGATGCCGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCAAACATGACCatcatc > 1:277080/1‑140 (MQ=255) tAACGATGCCGCCGTTGGTGTTGTAACGATAAC‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCAtcatca < 1:166229/140‑1 (MQ=255) aaCGATGCGGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCaa < 1:446770/140‑1 (MQ=255) aggccgccGTTGGTGTTGTAACGAAAAC‑CGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAg < 2:708946/138‑1 (MQ=255) aTGCCGCCGTTGGTGTTGTAACGAAAAC‑CGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAg < 2:571796/140‑1 (MQ=255) ccgTTGGTGTTGAAAC‑ATAAACCGCGCCCCGACCCCCCATTAGCCATATCGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATc < 2:277080/140‑1 (MQ=255) gtttGGTGTTGTAACAAAAAC‑CGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATcc < 2:441044/138‑1 (MQ=255) ttGGTTTTGTAACGAAACC‑CGCCCCCCGCCCCGCCAGTAGCCATAACGCCCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAg < 2:323309/140‑1 (MQ=255) aaa‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCa > 1:566600/1‑140 (MQ=255) aCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCt > 2:29424/1‑140 (MQ=255) cACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATc > 1:657558/1‑140 (MQ=255) tAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAAt > 1:68209/1‑140 (MQ=255) ccATAACGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGc > 1:464732/1‑140 (MQ=255) aaCGACCGCGTTAGCTCGGATCAGCGCCAACGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCagag < 1:343231/140‑1 (MQ=255) aaCGACCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCagag < 1:16752/140‑1 (MQ=255) aCCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGt < 1:825920/140‑1 (MQ=255) aCCGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGt < 2:657558/140‑1 (MQ=255) ccGCGTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAAAGCTAGAGCTGCGGGAATTGCAGAGAGGTc > 1:236871/1‑140 (MQ=255) gTTAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGa < 2:767912/140‑1 (MQ=255) ttAGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAGAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGaa > 1:522644/1‑140 (MQ=255) aGCACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACa < 1:131320/140‑1 (MQ=255) cACGGATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGc < 1:774170/140‑1 (MQ=255) ggATCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCgtg < 2:566600/140‑1 (MQ=255) aTCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGtgtg < 2:642929/140‑1 (MQ=255) aTCTGCACCAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGtgtg < 2:68209/140‑1 (MQ=255) aTCTGCACCAGCGTACCTTCCACCATGTTCATTGCCACCCGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGtgtg < 2:264339/140‑1 (MQ=255) aTCTGCACCAGCGTACCCTCCACCATGTTCATTGCCACCCGGCCGCGAGCATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGtgtg < 2:226655/140‑1 (MQ=255) cAGCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCatat < 1:29424/140‑1 (MQ=255) gCGTACCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATgg > 1:170358/1‑140 (MQ=255) | AACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAA‑CGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGG > NC_000913/4381653‑4381917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |