Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,811	A→G	intergenic (+153/‑81)	glyY → / → yjeV	tRNA‑Gly/uncharacterized protein YjeV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,811	0	A	G	100.0%	138.7 / NA	40	intergenic (+153/‑81)	glyY/yjeV	tRNA‑Gly/uncharacterized protein YjeV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/14);  total (26/14)

AAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGT  >  NC_000913/4392675‑4392943                                                                                                                                         |                                                                                                                                     aaaTATCCACAGCGACGCGATGCGTCATTACTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCt                                                                                                                                   <  1:352872/140‑1 (MQ=255)     atCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGTAACTTCGCACGCTGGATAGTAGTTTTTTAGCCTATTg                                                                                                                               >  1:255789/1‑140 (MQ=255)       ccACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTgat                                                                                                                             >  1:579563/1‑140 (MQ=255)           aGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTgatagat                                                                                                                         <  1:47772/140‑1 (MQ=255)                cgcgATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGtt                                                                                                                    >  1:90882/1‑140 (MQ=255)                                 ggTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGt                                                                                                   >  2:9492/1‑140 (MQ=255)                                 ggTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGt                                                                                                   >  1:655606/1‑140 (MQ=255)                                  gTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGtt                                                                                                  >  2:350913/1‑140 (MQ=255)                                    ttttGTTGTCTCTGACAAACTCTTGCAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGtttt                                                                                                <  1:350913/140‑1 (MQ=255)                                            tctcTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTgtcgagtc                                                                                        >  2:481914/1‑140 (MQ=255)                                                      aCTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGa                                                                              <  2:90882/140‑1 (MQ=255)                                                          ttGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGAt                                                                          >  2:61841/1‑140 (MQ=255)                                                            gTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCTGTTATGGCGAGTCGATCACTTGACGAttt                                                                        >  1:250404/1‑140 (MQ=255)                                                               aaCAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTAt                                                                     <  2:579563/140‑1 (MQ=255)                                                               aaCAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTAt                                                                     <  1:481914/140‑1 (MQ=255)                                                                  agagTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCg                                                                  >  1:761873/1‑140 (MQ=255)                                                                    agTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTc                                                                >  1:729920/1‑140 (MQ=255)                                                                            acaGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTTTCGTGTCACTCACTTGCCGATCCTATTCGTCTTGAATTg                                                        >  2:395198/1‑140 (MQ=255)                                                                              aGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCa                                                      >  2:689249/1‑140 (MQ=255)                                                                              aGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCa                                                      <  2:729920/140‑1 (MQ=255)                                                                              aGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTAACGATTTTAGTCGTCTTGAATTGCa                                                      >  1:216159/1‑140 (MQ=255)                                                                                   cAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTc                                                                   >  2:673843/1‑122 (MQ=255)                                                                                   cAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTc                                                                   <  1:673843/122‑1 (MQ=255)                                                                                          tAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGATTCGATCACATGAAGATTTTATTCGTGTTGATTTGCAATGCGtttttat                                          >  2:228000/1‑140 (MQ=255)                                                                                          tAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGtttttat                                          >  1:122763/1‑140 (MQ=255)                                                                                          tAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGtttttat                                          >  1:118116/1‑140 (MQ=255)                                                                                            aTCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGtttttattt                                        >  2:419703/1‑140 (MQ=255)                                                                                             tCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGtttttatttt                                       <  2:655606/140‑1 (MQ=255)                                                                                                tAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTttttatttttat                                    >  1:803024/1‑140 (MQ=255)                                                                                                tAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTttttatttttat                                    >  1:29278/1‑140 (MQ=255)                                                                                                tAATTTCAAAGAAACCTCGCACCGTGAATCGTATTTTATTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTttttatttttat                                    <  1:228000/140‑1 (MQ=255)                                                                                                    ttCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTcac                                >  1:203902/1‑140 (MQ=255)                                                                                                    ttCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTca                                 >  2:194639/1‑139 (MQ=255)                                                                                                    ttCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTca                                 <  1:194639/139‑1 (MQ=255)                                                                                                       aaaGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAg                             <  2:761873/140‑1 (MQ=255)                                                                                                        aaGAAACTTCGCACGGTTAATACTATTTTTTCAGCCTATTGATAGATACGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGc                            <  2:216159/140‑1 (MQ=255)                                                                                                        aaGAAACTTCGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGc                            >  1:763411/1‑140 (MQ=255)                                                                                                                 cGCACGGTGAATAGTATTTTTTTAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGa                   >  2:479752/1‑140 (MQ=255)                                                                                                                   cACGGTGAATAGTATTTTTTTAGCCGATTGATAGATACGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAAt                 <  1:395198/140‑1 (MQ=255)                                                                                                                                  ttttttAGCCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTGTATTCACAAGCAGTCGATGAAGCAGGCGTCACGCGGt  >  1:376838/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     AAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGT  >  NC_000913/4392675‑4392943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑