| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,423,375 | A→G | N177S (AAT→AGT) | ulaE → | L‑ribulose‑5‑phosphate 3‑epimerase UlaE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,423,375 | 0 | A | G | 95.7% | 144.7 / ‑2.6 | 47 | N177S (AAT→AGT) | ulaE | L‑ribulose‑5‑phosphate 3‑epimerase UlaE |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (24/21); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCA > NC_000913/4423240‑4423506 | gtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCg < 2:804244/140‑1 (MQ=255)gtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCg < 2:690286/140‑1 (MQ=255) gTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAg > 1:750852/1‑140 (MQ=255) agCGTTGAGATGGCAAGCCGCGTGCTGGTTACGCTGGTGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCgg < 1:306672/140‑1 (MQ=255) agCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATc > 2:407446/1‑138 (MQ=255) agCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATc < 1:407446/138‑1 (MQ=255) agCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTAAACAGTCCGTGGTTCCAACTCTACCCGGATATCgg > 2:309982/1‑140 (MQ=255) cGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCa > 1:132271/1‑140 (MQ=255) gTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCaa > 2:445488/1‑140 (MQ=255) ttGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAAc > 1:808397/1‑140 (MQ=255) ttGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCACACTATCTTAACAGTCTGTGGTTCCAGCTCTACCCGGATATCGGCAAc > 2:306672/1‑140 (MQ=255) gCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGc > 2:586395/1‑140 (MQ=255) cAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCg < 2:132271/140‑1 (MQ=255) aaGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGt < 1:445488/140‑1 (MQ=255) cgcgcgCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGa < 2:750852/140‑1 (MQ=255) cgcgcAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACa < 2:600990/140‑1 (MQ=255) cgcgcAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCACTAGCCGGATATCGGCAAGCTGTCGGGGTGGGACa > 2:393054/1‑140 (MQ=255) aGGTGACGCTGGCGACGGAGATCATGGATTATCCGTTAATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGAt < 1:372406/140‑1 (MQ=255) aTTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATc > 1:527537/1‑140 (MQ=255) aTTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCATGGTAGATCGATGTGCAGATGTAGTTGCAGGCCGGAATc > 2:370681/1‑140 (MQ=255) aTCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCggg > 1:386/1‑140 (MQ=255) tCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGGCGGCGTGGGACAGCGAGGTGCAGATTGAGTGGCAGGCCGGAATCGGGc > 2:223644/1‑140 (MQ=255) tgatgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGc < 2:586462/124‑1 (MQ=255) tgatgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGc > 1:586462/1‑124 (MQ=255) atgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAAt < 1:436817/128‑1 (MQ=255) atgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAAt > 2:436817/1‑128 (MQ=255) atgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgt > 2:551701/1‑140 (MQ=255) gaGCTCCATCAGCAATGCGCTGTGATACGAGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgtcg < 1:223644/140‑1 (MQ=255) gaGCTCCATCAGCAATGCGCTGTGAAACGCGCACTATTTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgtcg < 1:393054/140‑1 (MQ=255) gaGCTCCATCAGCAAGGTGCTGTGATACGCGCACTATCTTAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgtcg < 2:265133/140‑1 (MQ=255) cTCCATCAGCAAGGCACTGGGATACTCGCTCTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCgg < 1:309982/140‑1 (MQ=255) cTCCATCAGCAAGGCACTGGGACACTCGCCCTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCgg < 1:378875/140‑1 (MQ=255) tCCATCAGCAAGGCGATGGGATTCGGGCATTATCTCATCAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGt < 1:370681/140‑1 (MQ=255) tCCATCAGAAAGGCGCTGGGATGCGCGCCCGATTTTAACGGTCCGTGGCTCCAGCTCTCCCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCGGGAATCGGGCATATCGCCGCGGt < 2:403350/140‑1 (MQ=255) cATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTAc > 1:564253/1‑140 (MQ=255) cATCAGCAAGGCGCTGGGATACCCGCACTATGTCACCAGCCCGTGGTTGCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTAc < 2:295454/140‑1 (MQ=255) gCAAGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACAtgtg < 1:586395/140‑1 (MQ=255) aaGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGaa > 2:143706/1‑140 (MQ=255) aGGCGCTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGaaa < 1:68423/140‑1 (MQ=255) cgcTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGaaa > 1:487279/1‑137 (MQ=255) cgcTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGaaa < 2:487279/137‑1 (MQ=255) cTGGGATACGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGacac > 2:743864/1‑140 (MQ=255) aCGCGCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTTAAAGACACCAAACCt > 2:54357/1‑140 (MQ=255) cgcgCACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTg < 2:564253/140‑1 (MQ=255) gcACTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCAGATCGTCGAGGTTCAAGTGAAAGACACCAAACCTGGCg > 1:289990/1‑140 (MQ=255) aCTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTc > 1:720431/1‑140 (MQ=255) cTATCTCAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCt > 1:621188/1‑140 (MQ=255) tctcAACAGTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTTGCGGTACATGTCAACGACACCTAACCTGGCGTCTTCa > 1:376878/1‑140 (MQ=255) | GTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCA > NC_000913/4423240‑4423506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |