Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,514,148	A→G	V54A (GTG→GCG)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,514,148	0	A	G	100.0%	77.2 / NA	23	V54A (GTG→GCG)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/9);  total (14/9)

GTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGC  >  NC_000913/4514011‑4514277                                                                                                                                          |                                                                                                                                  gTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCcgcg                                                                                                                                 >  1:713098/1‑140 (MQ=255)              aaTGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGTATGACGTCCGCGGCGGCCAGCGCAt                                                                                                                    <  2:555383/140‑1 (MQ=255)                         ggCTCGGCTGCGCGCGCATTCCTACGGACTGGCACTGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACgag                                                                                                         <  2:320475/140‑1 (MQ=255)                            tCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGt                                                                                                      >  1:681125/1‑140 (MQ=255)                              ggCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTc                                                                                                    >  2:57804/1‑140 (MQ=255)                               gCTGCGCGCGCGTTCCGACGGACTGTCACGGTTTCAGGTGCGCACGCACGTCGGGCAGGATGCGGTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCTGCGACCAGCGCATCGGCGAACGAGAGTTcc                                                                                                   >  2:823963/1‑140 (MQ=255)                                 tGCGCGCGCGTTCCGACGGACTGACACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAg                                                                                                 <  2:713098/140‑1 (MQ=255)                                                      tGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGt                                                                            >  1:443940/1‑140 (MQ=255)                                                                      gcgcACGCACTTCGGGCAGGATGCGTTTTGTATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAAc                                                            >  2:773489/1‑140 (MQ=255)                                                                              acTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACACTCCGTTGAGGCGTTGTTTCGAGTGTAAACGTGCCgtg                                                    >  1:225644/1‑140 (MQ=255)                                                                              acTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCgtg                                                    >  2:688203/1‑140 (MQ=255)                                                                                         gATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGaa                                         <  2:443940/140‑1 (MQ=255)                                                                                                                 ggCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGa                 <  2:681125/140‑1 (MQ=255)                                                                                                                 ggCAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTCTAAACGTGGCGTGTTCATCCTGAACAGTGGCGGCAAAGGCGTGGCTGa                 >  2:249999/1‑140 (MQ=255)                                                                                                                   cAATACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATc               >  1:637997/1‑140 (MQ=255)                                                                                                                      tACCGATCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGaccacc            <  1:557215/140‑1 (MQ=255)                                                                                                                      tACCGATCGGGATGACGCCCGCGGCGGCCAGGGCAACGGCGAACGAGAATTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCAcc            <  2:305722/140‑1 (MQ=255)                                                                                                                      tACCGATCGGGATGAAGTCCGCCGCTGCCAGCGCATCCGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCAcc            <  1:249999/140‑1 (MQ=255)                                                                                                                           aTCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACcagcag       >  1:627925/1‑140 (MQ=255)                                                                                                                            tCGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACcagcagc      >  2:26332/1‑140 (MQ=255)                                                                                                                             cGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTg                  <  2:158710/127‑1 (MQ=255)                                                                                                                             cGGGATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTg                  >  1:158710/1‑127 (MQ=255)                                                                                                                                gATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCTGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGc  >  1:628211/1‑140 (MQ=255)                                                                                                                                gATGACGTCCGCGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGc  >  2:795750/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  GTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGC  >  NC_000913/4514011‑4514277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑