Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	525,807	C→T	T849T (ACC→ACT)	rhsD →	protein RhsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	525,807	0	C	T	97.0%	89.0 / ‑4.1	33	T849T (ACC→ACT)	rhsD	protein RhsD
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (13/19);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

TACCTGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACCCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGACGCGGGAATACGGCTACAGCGCCACGGGC  >  NC_000913/525673‑525942                                                                                                                                       |                                                                                                                                        tACCTGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGc                                                                                                                                    <  1:194217/140‑1 (MQ=255)   ccTGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAg                                                                                                                                  <  2:476348/140‑1 (MQ=255)     tGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGc                                                                                                                                <  2:711124/140‑1 (MQ=255)     tGGCGGGAATGAAGCGGGGCGGTACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGc                                                                                                                                <  1:371055/140‑1 (MQ=255)             aTGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTACACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACa                                                                                                                        <  1:706882/140‑1 (MQ=255)                  gCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTATAGAGcc                                                                                                                   <  1:15943/140‑1 (MQ=255)                  gCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGcc                                                                                                                   <  2:811564/140‑1 (MQ=255)                  gCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGcc                                                                                                                   <  1:72765/140‑1 (MQ=255)                    tGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGGGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAg                                                                                                                 >  2:760592/1‑140 (MQ=255)                        cGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATATACTCCCGCAGGCCCGTTACAGAGCCAGCAcc                                                                                                             >  2:250793/1‑140 (MQ=255)                           gACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGa                                                                                                          <  2:807911/140‑1 (MQ=255)                            aCGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAg                                                                                                                 >  1:423420/1‑132 (MQ=255)                            aCGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAg                                                                                                                 <  2:423420/132‑1 (MQ=255)                            aCGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGaa                                                                                                         <  1:493576/140‑1 (MQ=255)                                              aCGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCg                                                                                       <  2:178709/140‑1 (MQ=255)                                                           tGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTgg                                                                          <  2:545274/140‑1 (MQ=38)                                                                 gTGAGACGGTGCGCAGCTTCGGGAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGAc                                                                    <  2:807495/140‑1 (MQ=255)                                                                  tGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCAAGCACCTGCACAGCATTTTATATGACCGTGAATACGGGTGGAGTGACa                                                                   >  2:401175/1‑140 (MQ=14)                                                                          tGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGAc                                                           >  1:673503/1‑140 (MQ=255)                                                                          tGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACCCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGAc                                                           >  1:439066/1‑140 (MQ=40)                                                                              cAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTgg                                                       >  2:35387/1‑140 (MQ=255)                                                                              cAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTgg                                                       <  2:673503/140‑1 (MQ=255)                                                                                      gcagcaTGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATc                                               >  2:69906/1‑140 (MQ=255)                                                                                           aTGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCgg                                          >  1:187339/1‑140 (MQ=255)                                                                                             ggcaggcaGTAATGCCGCATACTAACTGATCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCGGcc                                        <  1:250793/140‑1 (MQ=255)                                                                                                      tAATGCCGCATACGAACTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGa                               >  1:429241/1‑140 (MQ=255)                                                                                                                  cGAACTGACCAGCACTTACACTGCCCCAGTCCAGTTAGAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGACGCGGGAATACg                   <  1:401175/140‑1 (MQ=11)                                                                                                                    aaCTGACCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGACGCGGGAATACGGc                 <  1:35387/140‑1 (MQ=21)                                                                                                                         aCCAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCCTCAGCGGACCGAGAAAGACGCCGGAATACGGCTACAg            >  1:291833/1‑140 (MQ=11)                                                                                                                           cAGCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGACGCGGGAATACGGCTACAgcg          >  2:179510/1‑140 (MQ=21)                                                                                                                             gCACATACACTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTAGTGCGCATGAGCTGCCAACGACAGACGCGGGAATACGGCGACAGCGcc        >  2:399358/1‑140 (MQ=11)                                                                                                                             gCACATACACTCCCGCACGCCAATTACAGAGACAGTACCCGAACAGCCGGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGACGCGGGAATACGGCTACAGCGcc        <  2:297127/140‑1 (MQ=11)                                                                                                                                   acacTCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGACGCGGGAATACGGCTACAGCGCCACGGGc  >  1:756084/1‑140 (MQ=21)                                                                                                                                       |                                                                                                                                        TACCTGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTCGAGTATACGCGGGACAGGCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATACGAACTGACCAGCACATACACCCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTATATGACCGTGACTACGGGTGGAGTGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGACGCGGGAATACGGCTACAGCGCCACGGGC  >  NC_000913/525673‑525942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑