Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,562,396	A→G	L94L (TTG→CTG)	yjiJ ←	putative transporter YjiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,562,396	0	A	G	100.0%	160.2 / NA	46	L94L (TTG→CTG)	yjiJ	putative transporter YjiJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/20);  total (26/20)

CAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAA  >  NC_000913/4562261‑4562531                                                                                                                                        |                                                                                                                                        caAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCg                                                                                                                                     <  1:120215/140‑1 (MQ=255) aaaaaGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCg                                                                                                                                     <  2:488180/139‑1 (MQ=255)    aaGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTc                                                                                                                                  >  2:168148/1‑140 (MQ=255)            cgcgTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAACTGGCGGCACCCACGCCCTTGCGAGGCTCAACAGCCCGCTCACCAGGGc                                                                                                                          >  2:250237/1‑140 (MQ=255)            cgcgTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGc                                                                                                                          >  1:200668/1‑140 (MQ=255)            cgcgTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGc                                                                                                                          >  1:680222/1‑140 (MQ=255)                  tgctgcATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGc                                                                                                                    <  2:94754/140‑1 (MQ=255)                     tgcATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCACCAGGGCAGaa                                                                                                                      >  2:41217/1‑135 (MQ=255)                     tgcATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCACCAGGGCAGaa                                                                                                                      <  1:41217/135‑1 (MQ=255)                                 gTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGtcg                                                                                                     >  1:408468/1‑140 (MQ=255)                                         aaaaaTCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGTCGCAGGcgcg                                                                                             <  2:408468/140‑1 (MQ=255)                                         aaaaaTCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGcgcg                                                                                             <  2:200668/140‑1 (MQ=255)                                         aaaaaCCAGTATACCGGCGCTGCGGCCACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGcgcg                                                                                             <  1:23534/140‑1 (MQ=255)                                           aaaTCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGAt                                                                                           <  1:431436/140‑1 (MQ=255)                                                         gcgcTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATgc                                                                             >  1:189623/1‑140 (MQ=255)                                                           gcgggCGACTGCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATgcgc                                                                           <  1:112832/137‑1 (MQ=255)                                                              ggCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCaa                                                                        >  2:42139/1‑140 (MQ=255)                                                               gCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAACCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCaaa                                                                       >  2:558257/1‑140 (MQ=255)                                                                  acacCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGa                                                                    <  1:42139/140‑1 (MQ=255)                                                                       cGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGATGGTGAAATACGCCAAAAGAAAATa                                                               >  1:339190/1‑140 (MQ=255)                                                                         ccAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGc                                                             >  2:755355/1‑140 (MQ=255)                                                                         ccAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGc                                                             >  2:13223/1‑140 (MQ=255)                                                                            ggACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGa                                                          <  1:755355/140‑1 (MQ=255)                                                                                              ccAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTCCCAGCCAGATAcccc                                        >  1:317222/1‑140 (MQ=255)                                                                                                aGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGGCAAAAGAAAATAGCGGACTGCCAGCCAGATACCCCGc                                      >  1:353389/1‑140 (MQ=255)                                                                                                 gAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGAt                                             <  1:450883/132‑1 (MQ=255)                                                                                                 gAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGAt                                             >  2:450883/1‑132 (MQ=255)                                                                                                 gAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCa                                     >  2:59276/1‑140 (MQ=255)                                                                                                  aaTAAATGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCAt                                    >  2:752381/1‑140 (MQ=255)                                                                                                       aTGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCTGACAGCTAGCCAGGTACCCCGCATAGTTg                               >  1:224422/1‑140 (MQ=255)                                                                                                       aTGGCGGCAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTg                               >  2:533770/1‑140 (MQ=255)                                                                                                         ggcggcAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGTCTGGCATCCAGATCCCCCGCATAGTTGcc                             >  1:285937/1‑140 (MQ=255)                                                                                                         ggcggcAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGcc                             >  2:107986/1‑140 (MQ=255)                                                                                                          gcggcAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCg                            <  1:168148/140‑1 (MQ=255)                                                                                                            ggcAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATGGCAGACTTCCAACCGGATACCGCGCATAGTTGCCGCt                          >  1:223110/1‑140 (MQ=255)                                                                                                            ggcAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGTAAATTGCCAGCCTGATAATCCGCATAATTGCCGCt                          >  2:377962/1‑140 (MQ=255)                                                                                                              cAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTgg                        <  2:798504/140‑1 (MQ=255)                                                                                                              cAACCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTgg                        <  1:752381/140‑1 (MQ=255)                                                                                                                aCCATGCCATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCa                      >  1:117415/1‑140 (MQ=255)                                                                                                                      ccATTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCAc                <  1:533770/140‑1 (MQ=255)                                                                                                                        aTTGCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACga              >  1:636781/1‑140 (MQ=255)                                                                                                                           gCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGagag           <  2:117415/140‑1 (MQ=255)                                                                                                                           gCGAGGATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGagag           >  1:669812/1‑140 (MQ=255)                                                                                                                               ggATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTgg       <  2:636781/140‑1 (MQ=255)                                                                                                                               ggATCAACAGCCCGCTCGCCAGGGCAGAAACCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTgg       <  1:558257/140‑1 (MQ=255)                                                                                                                                gATCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGc      <  2:60064/140‑1 (MQ=255)                                                                                                                                 aTCAACAGCCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCt     >  2:588579/1‑140 (MQ=255)                                                                                                                                    aacagcCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTaaa  <  1:588579/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAA  >  NC_000913/4562261‑4562531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑