Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,567,017	A→G	I77T (ATC→ACC)	yjiN ←	DUF445 domain‑containing protein YjiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,567,017	0	A	G	100.0%	69.0 / NA	21	I77T (ATC→ACC)	yjiN	DUF445 domain‑containing protein YjiN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/13);  total (8/13)

GCTGGCTAAACCAGTTGCCAATCAGCAACGCCGGTTCGTGGCGTCGAATCAATGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGC  >  NC_000913/4566883‑4567151                                                                                                                                       |                                                                                                                                       gctggctAAACCAGTTGCCAATCAGCAACGCCGGTTCGTGGCGTCGAATCAATGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATc                                                                                                                                   >  2:5856/1‑140 (MQ=255)      ctAAACCAGTTGCCAATCAGCAACGCCGGTTCGTGGCGTCGAATCAATGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGt                                                                                                                              <  2:616599/140‑1 (MQ=255)           ccAGTTGCCAATCAGCAGCGCCTGTTCGTGGGGTCGAATGAATGCCACGAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGc                                                                                                                         <  2:356082/140‑1 (MQ=255)           ccAGTTGCCAATCAGCAACGCCGGTTCGTGGCGTCGAATCAATGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGc                                                                                                                         <  2:61891/140‑1 (MQ=255)                                cGGTTCGTGGCGTCGCATCAATTCCGCCAGGGATTGAATATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCa                                                                                                    <  1:314462/140‑1 (MQ=255)                                    tCGTGGCGTCGAATCAATGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACAAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCAcgcg                                                                                                >  1:469736/1‑140 (MQ=255)                                            tCGTATCAATGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACa                                                                                        >  2:35212/1‑140 (MQ=255)                                              gAATCAATGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAgc                                                                                      <  2:708718/140‑1 (MQ=255)                                                aTCAATGCCACCAGGGTATGAGAATCAAGAATTGTATCCTGCACGAACAGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAgcgc                                                                                    <  2:375266/140‑1 (MQ=255)                                                aTCAATGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAgcgc                                                                                    >  2:749597/1‑140 (MQ=255)                                                   aaTGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGccac                                                                                 <  2:206075/140‑1 (MQ=255)                                                   aaTGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGccac                                                                                 <  2:727604/140‑1 (MQ=255)                                                   aaTGCCACCAGGGATTGAGTATAAATAAACTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGccac                                                                                 <  1:276139/140‑1 (MQ=255)                                                                  tGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGATACTGCGCCATCACCGCAAACCAGTc                                                                  >  1:384494/1‑140 (MQ=255)                                                                        tCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAg                                                            >  1:447998/1‑140 (MQ=255)                                                                         cAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGCGGTAATCGCCGTATGGTGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAgc                                                           <  2:469736/140‑1 (MQ=255)                                                                                          aCGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATcgccgc                                          >  1:522045/1‑140 (MQ=255)                                                                                                            tCGCCAATCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCAc                        <  2:100377/140‑1 (MQ=255)                                                                                                                 aaTCCGGTCTTTATTACGCGGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCACCGACCATCGCCGCGTCAGCAATCGCCTTCAcgccgc                   >  1:318463/1‑140 (MQ=255)                                                                                                                                 cgcgGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTggg   <  1:35212/140‑1 (MQ=255)                                                                                                                                  gcgGGGTAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGggc  <  2:522045/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GCTGGCTAAACCAGTTGCCAATCAGCAACGCCGGTTCGTGGCGTCGAATCAATGCCACCAGGGATTGAGTATCAAGAAATTTTTCCTGCACGAACTGGCCGAGATTTTCGCCAATCCGGTCTTTATTACGCGGGATAATCGCCGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGC  >  NC_000913/4566883‑4567151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑