Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,568,451	A→G	F23F (TTT→TTC)	mdtM ←	multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,568,451	0	A	G	100.0%	183.0 / NA	52	F23F (TTT→TTC)	mdtM	multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/16);  total (36/16)

CCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGA  >  NC_000913/4568315‑4568584                                                                                                                                         |                                                                                                                                       ccAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAg                                                                                                                                     <  2:815589/140‑1 (MQ=255)        ccACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACa                                                                                                                              <  2:17128/140‑1 (MQ=255)         cACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACaa                                                                                                                             >  2:678353/1‑140 (MQ=255)                    cATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCa                                                                                                                  >  1:700841/1‑140 (MQ=255)                      tACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGTCAGATACTCAGAGAGGTCATAAAAAATCAACGCCATc                                                                                                                >  1:232894/1‑140 (MQ=255)                      tACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATc                                                                                                                >  1:77131/1‑140 (MQ=255)                                     agagACTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTg                                                                                                 >  1:695832/1‑140 (MQ=255)                                       agaCTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAGAATCAACGCCATCGGGAAAAACAGCGTggc                                                                                               >  1:525687/1‑140 (MQ=255)                                         aCTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTggcgg                                                                                             >  2:575391/1‑140 (MQ=255)                                             aCGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATg                                                                                         >  2:497765/1‑140 (MQ=255)                                             aCGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATg                                                                                         >  2:190052/1‑140 (MQ=255)                                             aCGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATg                                                                                         >  2:18055/1‑140 (MQ=255)                                               ggCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGc                                                                                       <  1:829455/140‑1 (MQ=255)                                                   gcagGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGt                                                                                   >  2:447190/1‑140 (MQ=255)                                                            aGACTGACATCGGCAGTAAAATAACGTACAACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg                                                                          <  1:326812/140‑1 (MQ=255)                                                              aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAGAATCACCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTg                                                                        <  2:525687/140‑1 (MQ=255)                                                                 gACATCGGCATTAAACTCACGTTCCCCATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCa                                                                     <  1:400992/140‑1 (MQ=255)                                                                  aCATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCAt                                                                    >  1:811456/1‑140 (MQ=255)                                                                   cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGTGGCATa                                                                   >  1:71278/1‑140 (MQ=255)                                                                    aTCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaa                                                                  <  2:695832/140‑1 (MQ=255)                                                                    aTCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaa                                                                  <  1:678353/140‑1 (MQ=255)                                                                        gCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaaaaaa                                                              >  1:681190/1‑140 (MQ=255)                                                                                         cacaTTAATGATCCCAGGCTGGCTCTGATCCGTCGACTGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGgag                                             <  1:399620/140‑1 (MQ=255)                                                                                           caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGGAAATAAAGAGGCATAAAAAAAAACACCTTGTCCGGAGaa                                           >  2:308456/1‑140 (MQ=255)                                                                                              tAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAATGGCATAAAAAACAACACCTTGTCCGGAGAA‑ccc                                       >  2:734458/1‑140 (MQ=255)                                                                                               aaTGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGGGGGCGAAATAAAGCGGGCCTACAAACAAATACCTGTTCGGAGGA‑ccgc                                      >  2:328539/1‑138 (MQ=255)                                                                                                   aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGGATAACAAAAAACCCCCTGTGCGGAGAA‑ACCCCAcc                                  >  2:152416/1‑139 (MQ=255)                                                                                                   aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGGATAACAAAAAAATCCTTGTCCGGGGAA‑CCCCCACg                                  >  2:122585/1‑140 (MQ=255)                                                                                                   aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGAAAAAAAAATCGGTATAAAAAACAACTCCCTTTTCGGAGAA‑CCCCAAcc                                  >  2:314136/1‑139 (MQ=255)                                                                                                      ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACgggg                               >  1:657849/1‑140 (MQ=255)                                                                                                      ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGGGGGATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACgggg                               >  1:421137/1‑140 (MQ=255)                                                                                                      ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACgggg                               >  1:459990/1‑140 (MQ=255)                                                                                                      ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCAAAAAAAAAAAACCCTTTTCCGGGGAACCCCCAACggg                                >  2:704386/1‑140 (MQ=255)                                                                                                         ggCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAACAAATCCTTTTCCGGAGAA‑CCCCAACGGGGgaa                            >  1:668295/1‑140 (MQ=255)                                                                                                          gCTGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAAAAAATCCTTGTCCGGGGAA‑CCCCCAAGGGGgaaa                           >  2:656088/1‑140 (MQ=255)                                                                                                            tGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACg                         <  1:122585/140‑1 (MQ=255)                                                                                                            tGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACg                         <  2:45154/140‑1 (MQ=255)                                                                                                            tGGATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACg                         <  1:447190/140‑1 (MQ=255)                                                                                                              gATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGaa                       <  1:497765/140‑1 (MQ=255)                                                                                                              gATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACAGGGCATAAAAAAAAACCCCTTTTCCGGGGGA‑ACCCCACGGGGaaaaagga                       >  1:830578/1‑136 (MQ=255)                                                                                                              gATCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGCAAAAAAAACGGCATAAAAAAACACCTCTTATCCGGAGCA‑ACCCCACGGGGAAAACGaa                       >  1:520988/1‑140 (MQ=255)                                                                                                               aTCAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCAAAAAAAACAACTCCTTTTGCGGAGAA‑ACCCAACCGGGGAAACGaaa                      >  1:788892/1‑140 (MQ=255)                                                                                                                 cAGATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCGTAAAAAACAAAACCGTATCCGGGGAA‑ACCCAGCGGGGGAAAAGaaaat                    >  2:355547/1‑138 (MQ=255)                                                                                                                   gATCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGAAATTCa                  <  1:734458/140‑1 (MQ=255)                                                                                                                    aTCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGAAATTCaa                 <  2:681190/140‑1 (MQ=255)                                                                                                                    aTCCGTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGGAAACGAAAATCaa                 >  1:785505/1‑140 (MQ=255)                                                                                                                     tCCGTCGACAGATACCCAGCGAGGTCATAAAAAATCACCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGAAATTCAAt                <  1:308456/140‑1 (MQ=255)                                                                                                                        gTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGGGGCATAAAAAAAAACACCTTGTCCGGAGAA‑CCCCAACGGGGGAAAAGAAAATCAAAAcc             >  2:551195/1‑140 (MQ=255)                                                                                                                        gTCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCTTTTCCGGAGAA‑ACCCAACGGGGGAAAAGAAAATCAATAcc             >  1:201461/1‑140 (MQ=255)                                                                                                                         tCGACAGATACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCATAACAAACAAACCCTTGTCCGGGGAA‑ACCCAACGGGGGAAAAGAAAATCAATaacc            >  2:517040/1‑137 (MQ=255)                                                                                                                                aTACGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAACAACTCCTTTTCCGGAGAA‑ACCCCACGGGGAAAACGAAAATCAAAACCGCCGGTaa     >  2:521520/1‑140 (MQ=255)                                                                                                                                   cGCAGCGAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGGATAAAAAACAAAACCCTTTCCGGAGAA‑CCCCAAACGGGAAAAAGAAAATCCAAACCCTCGGGAAAGa  >  2:470132/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CCAGCAGCCACTGTAACGCCATACCGCCAGCAAGATAGAGACTGACGGCAGCAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGA  >  NC_000913/4568315‑4568584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑