| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,593,220 | C→T | R522C (CGT→TGT) | tsr → | methyl‑accepting chemotaxis protein Tsr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,593,220 | 0 | C | T | 94.7% | 55.5 / NA | 19 | R522C (CGT→TGT) | tsr | methyl‑accepting chemotaxis protein Tsr |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (8/10); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGTCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAA > NC_000913/4593087‑4593355 | tGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGTCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGaaa > 2:513763/1‑140 (MQ=255) cTCAACAGAACGCCGCGCTGGTCGAGGAGTCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAAcc < 1:302168/140‑1 (MQ=255) cAGAACGCCGCGCTGGTGGAAGAGTCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGAc > 2:701859/1‑140 (MQ=255) tggtggAAGAGTCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGcgccg < 1:178268/140‑1 (MQ=255) tgccgcCGCCGCGCTAGAAGAGTAGTCCATTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAg < 1:385827/140‑1 (MQ=255) ccgcGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCgag < 1:775258/140‑1 (MQ=255) ccgcGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCgag < 1:701859/140‑1 (MQ=255) ggCCAGTCACCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAAGGGCAGTGGCAGAGAGCGGGGAGAACTGGGAAACAt > 2:243001/1‑140 (MQ=255) cTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGcc < 2:659501/140‑1 (MQ=255) aGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAAt > 2:22915/1‑140 (MQ=255) gcagtgTTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGAt < 1:716576/140‑1 (MQ=255) gtgtTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAg > 1:557273/1‑140 (MQ=255) gtgtTCCGGATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAg < 2:557273/140‑1 (MQ=255) gtTCCGGATTCAGCAACAGCAGCGTCAAACATCGGCTTTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCa < 1:243001/140‑1 (MQ=255) gATTCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATTGCCATGCAAATGCGCGATAAGCAAAATGt > 1:214116/1‑140 (MQ=255) tCAGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTAt < 1:22915/140‑1 (MQ=255) aGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTCATCGCCAGGAATATACCCGATGAGCAAAATGTTATCg > 1:218559/1‑140 (MQ=255) aGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTAt < 1:42317/138‑1 (MQ=255) aGCAACAGCAGTGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTAt > 2:42317/1‑138 (MQ=255) gcagtGTGAAACATCGGTTGTGCTAAAAACCTTGAAGCCCGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATaa < 2:214116/140‑1 (MQ=255) gcagtGTGAAACATCGGCTGTGCTAAAAACCCTGACGCCCGCTGCGCCCCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATaa < 2:218559/140‑1 (MQ=255) | TGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGTCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAA > NC_000913/4593087‑4593355 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |