Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,595,207	A→G	D215D (GAT→GAC)	lgoR ←	putative DNA‑binding transcriptional regulator LgoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,595,207	0	A	G	100.0%	176.8 / NA	51	D215D (GAT→GAC)	lgoR	putative DNA‑binding transcriptional regulator LgoR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/22);  total (29/22)

ATCGACGGCAATGATATTGCGCTGTTTAAGATCGCTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAAT  >  NC_000913/4595072‑4595343                                                                                                                                        |                                                                                                                                         aTCGACGGCAATGATATTGCGCTGTTTAAGATCGCTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTCAGGAGTGAAAGtctc                                                                                                                                      >  1:291503/1‑140 (MQ=255) aTCGACGGCAATGATATTGCGCTGTTTAAGATCGCTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGtctc                                                                                                                                      >  1:604017/1‑140 (MQ=255) aTCGACGGCAATGATATTGCGCTGTTTAAGATCGCTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGtctc                                                                                                                                      >  1:744293/1‑140 (MQ=255)        gCAATGATATTGCGCTGGTTAATATCGCTTTCGTCTCATAGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAg                                                                                                                               <  2:291503/140‑1 (MQ=255)            tGATATTGCGCTGTTTAAGATCGCTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGc                                                                                                                           >  1:442971/1‑140 (MQ=255)                   gcgcTGTTTAAGATCGCTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACa                                                                                                                    <  2:779216/140‑1 (MQ=255)                                   cTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCGAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTAtt                                                                                                       >  2:524817/1‑137 (MQ=255)                                   cTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTAtt                                                                                                       <  1:524817/137‑1 (MQ=255)                                   cTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGcc                                                                                                    >  1:180116/1‑140 (MQ=255)                                   cTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGcc                                                                                                    >  1:741264/1‑140 (MQ=255)                                   cTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGcc                                                                                                    >  1:482155/1‑140 (MQ=255)                                   cTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGcc                                                                                                    >  2:142562/1‑140 (MQ=255)                                   cTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGcc                                                                                                    >  2:655261/1‑140 (MQ=255)                                   cTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGcc                                                                                                    >  2:710427/1‑140 (MQ=255)                                     ttCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGa                                                                                                  <  1:62958/140‑1 (MQ=255)                                            cATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTc                                                                                           >  2:447676/1‑140 (MQ=255)                                                       aaaaTGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGcc                                                                                >  1:633725/1‑140 (MQ=255)                                                              aaGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACg                                                                         <  1:710427/140‑1 (MQ=255)                                                                gATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGtt                                                                       <  2:436610/140‑1 (MQ=255)                                                                    aCTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCATGCAGTAGCCGATGACGTTCGAg                                                                   >  1:216489/1‑140 (MQ=255)                                                                        agaTGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGTAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATg                                                               >  1:190136/1‑140 (MQ=255)                                                                           tgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGtt                                                            >  1:493406/1‑140 (MQ=255)                                                                           tgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTG‑AAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGttt                                                           >  1:407588/1‑140 (MQ=255)                                                                             atCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCGATTGCCGAGGTTGTCACGCAGTAGCCGATGTCGTTCGAGCATGGtttt                                                          >  2:247574/1‑140 (MQ=255)                                                                             atCTCAAGCGATTGAGCAAAAATGAAATTCTCGGCAGTTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGtttt                                                          <  1:260796/140‑1 (MQ=255)                                                                                    gCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCAATGTTGTCACGCAGTAACCGATGACGTTCGGGCATGGTTTTCGCCTGc                                                   >  2:260796/1‑140 (MQ=255)                                                                                       aTTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagc                                                >  2:20774/1‑140 (MQ=255)                                                                                        ttGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagcc                                               >  2:143416/1‑140 (MQ=255)                                                                                            tCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCg                                           <  2:741264/140‑1 (MQ=255)                                                                                                 aaaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGAt                                      <  2:485610/140‑1 (MQ=255)                                                                                                          gTCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAGGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcag                             >  1:416835/1‑140 (MQ=255)                                                                                                           tCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcagg                            <  2:604017/140‑1 (MQ=255)                                                                                                           tCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcagg                            <  1:88292/140‑1 (MQ=255)                                                                                                           tCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcagg                            <  2:180116/140‑1 (MQ=255)                                                                                                           tCGGCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcagg                            <  2:633725/140‑1 (MQ=255)                                                                                                             ggCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcaggca                          >  1:665518/1‑140 (MQ=255)                                                                                                             ggCAGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcaggca                          <  2:216489/140‑1 (MQ=255)                                                                                                                aGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGt                       >  2:618423/1‑140 (MQ=255)                                                                                                                aGCTGAAAGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGt                       >  2:514065/1‑140 (MQ=255)                                                                                                                     aaaGCAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTgaga                  <  1:20774/140‑1 (MQ=255)                                                                                                                     aaaGCAGTAAGGAGTAAAAGTCGCTTTCAAGTTGCGAAAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTgaga                  <  1:283761/140‑1 (MQ=255)                                                                                                                         cAGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGt              >  2:137619/1‑140 (MQ=255)                                                                                                                          aGTAAGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTg             <  2:665518/140‑1 (MQ=255)                                                                                                                            tAAGGAGTG‑AAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTg          <  2:407588/140‑1 (MQ=255)                                                                                                                             aaGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCGGTAGACGATGAAGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGGTCATGATCGGGCAGGTTGAGAAAGTGCTg          <  1:201480/140‑1 (MQ=255)                                                                                                                             aaGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTg          <  1:522216/140‑1 (MQ=255)                                                                                                                              aGGAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGa         <  2:744293/140‑1 (MQ=255)                                                                                                                                gAGTGAAAGTCTCTGTCTAGTTGCGAGAACAGGGGAAAGCTATTGCCGGTGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAg       <  2:366291/140‑1 (MQ=255)                                                                                                                                gAGTGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAg       <  2:493406/140‑1 (MQ=255)                                                                                                                                   tGAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGa    >  1:440570/1‑140 (MQ=255)                                                                                                                                    gAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGGAGCCAGCGTGGAGCATGATCAGGCATGTTGCGAAAGTGCTGAAGTGaa   >  2:260059/1‑140 (MQ=255)                                                                                                                                    gAAAGTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTACCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGTATCATGAGCAGCCAAGTTGAGAAAGTGCTGAAGTGaa   >  1:248536/1‑140 (MQ=255)                                                                                                                                     aaagTCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAAt  <  1:618423/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         ATCGACGGCAATGATATTGCGCTGTTTAAGATCGCTTTCGTCCCATTGATAGTGAAAATGAAAGATGACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAAT  >  NC_000913/4595072‑4595343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑