Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,586	A→G	T194A (ACC→GCC)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,586	0	A	G	100.0%	165.0 / NA	49	T194A (ACC→GCC)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/23);  total (26/23)

TTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGT  >  NC_000913/4636452‑4636714                                                                                                                                       |                                                                                                                                 ttGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTc                                                                                                                             >  1:660372/1‑140 (MQ=255) ttGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTc                                                                                                                             >  1:31464/1‑140 (MQ=255) ttGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTc                                                                                                                             >  1:3110/1‑140 (MQ=255)         ccATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCca                                                                                                                     <  2:543928/140‑1 (MQ=255)                cgcTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGACGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTaaa                                                                                                              <  1:26859/140‑1 (MQ=255)                  cTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAAc                                                                                                            >  2:454657/1‑140 (MQ=255)                    gACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACAACTATGATCGCGCAGTCGATACCCACATTAAAACGc                                                                                                          >  2:276642/1‑140 (MQ=255)                     ccTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACGGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCt                                                                                                         <  2:100580/139‑1 (MQ=255)                      cTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGAGGCGCAGGATACCCATGCTCGCGCCGGCGATCACCACATTAAAACGCTg                                                                                                        >  2:354642/1‑140 (MQ=255)                          gTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGAAGGACACCGACGATCGTGCCGTCGAGACCCACATTAAAACGCTGCGTg                                                                                                    >  2:327780/1‑140 (MQ=255)                               aGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGGCGATACCCCCATTAAAACGCTGCGTGCCAAg                                                                                               >  2:244328/1‑140 (MQ=255)                                          ttgAAGACGTTACTCGAGTCCCGGGCCCGCGTCTGGTTCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCAt                                                                                    <  1:276642/140‑1 (MQ=255)                                          ttgAAGACGTTACTCAAGTCACCGGACCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCAt                                                                                    >  2:627580/1‑140 (MQ=255)                                           tgAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTTAAACGCAGCGTGCAAAGCTGCGCGCCATc                                                                                   >  1:272427/1‑140 (MQ=255)                                           tgAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATc                                                                                   >  1:698399/1‑140 (MQ=255)                                                aCGTTACTCAAGTCACCGGACCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAAccc                                                                              <  1:627580/140‑1 (MQ=255)                                                    tACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGAt                                                                          >  2:2812/1‑140 (MQ=255)                                                        cAAGTCCCCGGGCGGCGTCTGTTCCCGCCAGCAACAGATCGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCttt                                                                      <  2:272427/140‑1 (MQ=255)                                                          aGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATCGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCa                                                                    <  1:327780/140‑1 (MQ=255)                                                           gTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCAc                                                                   >  2:807925/1‑140 (MQ=255)                                                            tCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCAcc                                                                  <  1:807925/140‑1 (MQ=255)                                                              aCCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGa                                                                >  1:94775/1‑140 (MQ=255)                                                              aCCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGa                                                                >  2:524480/1‑140 (MQ=255)                                                              aCCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGa                                                                >  1:442275/1‑140 (MQ=255)                                                                cGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGAtt                                                              >  2:644990/1‑140 (MQ=255)                                                                    ccGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATa                                                          >  1:591110/1‑140 (MQ=255)                                                                     cgcgTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCTCCGATTAATAc                                                         >  1:34509/1‑140 (MQ=255)                                                                         tCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCAt                                                     >  1:703367/1‑140 (MQ=255)                                                                             gTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATcgcg                                                 <  1:524480/140‑1 (MQ=255)                                                                             gTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATcgcg                                                 <  1:454657/140‑1 (MQ=255)                                                                               cccGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGc                                               <  1:644990/140‑1 (MQ=255)                                                                                 cGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATTGCGGCAt                                             <  2:591110/140‑1 (MQ=255)                                                                                 cGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCAt                                             <  1:2812/140‑1 (MQ=255)                                                                                 cGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCAt                                             <  1:571171/140‑1 (MQ=255)                                                                                      gCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGat                                        <  2:94775/140‑1 (MQ=255)                                                                                      gCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGat                                        <  2:196431/140‑1 (MQ=255)                                                                                       cAACTGATGGATAACGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCTACCGTTCACCGATACATACTCATCGCGGCATGGGata                                       >  2:815316/1‑140 (MQ=255)                                                                                               ggATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCTAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGa                               <  2:3110/140‑1 (MQ=255)                                                                                                  tAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAggg                            >  2:742528/1‑140 (MQ=255)                                                                                                  tAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAggg                            <  1:742528/140‑1 (MQ=255)                                                                                                   aGCGTATGTGAAGATGAGCAGGGCACCTACCATCGCGCCTTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGTGGCATGGGATATAGCCTGAgggg                           <  2:375280/140‑1 (MQ=255)                                                                                                     cGTATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGcc                         >  2:430061/1‑140 (MQ=255)                                                                                                       tATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGa                               <  1:461951/132‑1 (MQ=255)                                                                                                       tATGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGa                               >  2:461951/1‑132 (MQ=255)                                                                                                         tGGGAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTa                     <  1:55472/140‑1 (MQ=255)                                                                                                           ggAAGATGCGCAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAAt                   <  1:467447/140‑1 (MQ=255)                                                                                                                     cAGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGc         >  2:578846/1‑140 (MQ=255)                                                                                                                      aGGACACCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCa        <  1:430061/140‑1 (MQ=255)                                                                                                                          cacCTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCg    >  1:2446/1‑140 (MQ=255)                                                                                                                            ccTACGATCGCGCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgt  <  2:660372/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 TTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGT  >  NC_000913/4636452‑4636714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑