| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 584,251 | T→C | Y191H (TAT→CAT) | appY → | DNA‑binding transcriptional activator AppY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 584,251 | 0 | T | C | 96.5% | 183.1 / ‑2.2 | 57 | Y191H (TAT→CAT) | appY | DNA‑binding transcriptional activator AppY |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (28/27); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01 | |||||||||||
TATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGA > NC_000913/584124‑584386 | tatTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAAc > 2:733708/1‑140 (MQ=255) gAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGATATATACTAGGCTGAGGCATGCAAAAAAACTCAt > 2:367869/1‑140 (MQ=255) gAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCAt > 1:140681/1‑140 (MQ=255) gAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCAt > 2:814425/1‑140 (MQ=255) gAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCAt > 1:100363/1‑140 (MQ=255) gAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCAt > 1:141239/1‑140 (MQ=255) gAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGAAACTCGGGTGAGGCGTGCAAAAAAACTCAt > 1:403292/1‑140 (MQ=255) cGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGGGAGATACGAGGGTGAGGCATGGAAAAAAACTCATAAc > 1:236086/1‑140 (MQ=255) gCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACtt > 2:408151/1‑140 (MQ=255) aaTGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCa < 2:718935/140‑1 (MQ=255) aaTGGCATTTAAAAGATATTGCAGAATTGATATATACGAGTGAAAGTTAAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCa < 2:299339/140‑1 (MQ=255) aTGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCaa > 2:607229/1‑140 (MQ=255) cATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAActc > 2:150777/1‑140 (MQ=255) ttAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTACGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTa < 2:499265/140‑1 (MQ=255) ttAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTa < 2:636805/140‑1 (MQ=255) ttAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTa < 1:476479/140‑1 (MQ=255) tAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGACAAGCAAAAAAACTCATAACTTCAAACTCTTAt > 2:398993/1‑140 (MQ=255) aaGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCt < 1:814425/140‑1 (MQ=255) aaGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCt > 1:702477/1‑140 (MQ=255) ggAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCg < 2:702477/140‑1 (MQ=255) ggAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCg < 1:733708/140‑1 (MQ=255) ggAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCg < 1:589680/140‑1 (MQ=255) ggAATTGATTTATACCAGTGAATGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCg < 1:367869/140‑1 (MQ=255) aaTTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTa < 2:141239/140‑1 (MQ=255) ttGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCg < 2:463295/136‑1 (MQ=255) ttGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCg > 1:463295/1‑136 (MQ=255) aTTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTACCAATGTCGTAGCCCa > 1:309831/1‑140 (MQ=255) taCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAGCTTGAAAATCTTATTCGATCAGTGTCGTAGCCCAGAAAt > 2:317891/1‑140 (MQ=255) taCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGaaac > 1:86649/1‑139 (MQ=255) gTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTTATAACTTCAAACTCTTTTTCTATCACTGTCCGAGCCCAGAAATGTGGc > 2:369487/1‑140 (MQ=255) gTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGc > 2:427349/1‑140 (MQ=255) gTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGc > 1:200607/1‑140 (MQ=255) tGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCt > 1:647118/1‑140 (MQ=255) aaaGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCtat < 2:140681/140‑1 (MQ=255) aGTTTAATTAAAAAAAGATTAACGGATGAATGATCTTCATTTACTGAATTATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATaa < 2:403292/140‑1 (MQ=255) aGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATaa < 1:468783/140‑1 (MQ=255) tttAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAAcg < 1:408151/140‑2 (MQ=255) ttAATAAAAAAAAGATTAAGGGTTGAAGGAACGTCATTTCCTGAAATAATGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAAcgg < 2:309831/140‑3 (MQ=255) aataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTa < 1:427349/140‑1 (MQ=255) aGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTa < 2:647118/140‑1 (MQ=255) aGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTa < 1:742363/140‑1 (MQ=255) aGGAACGTCATTTACTGAAATAGTGAGAGATACTAGGGTGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTa < 1:336334/140‑1 (MQ=255) gAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTaaa < 1:607229/140‑1 (MQ=255) gAACGTCATTTACTGAAATATTAAGAGATCCTAGGATTAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTaaa < 1:317891/140‑1 (MQ=255) cGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGat > 2:546573/1‑140 (MQ=255) tGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGgtg < 1:150777/140‑1 (MQ=255) aaaTATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTc > 1:791629/1‑140 (MQ=255) tatTGAGAGATACTAGGATGAGGCAAGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACg < 1:372982/140‑1 (MQ=255) agagaTACTAGGATGAGACATGTAAAAAAACGCATTACTTCAAACTCTAATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAtc < 2:236086/140‑1 (MQ=255) agaTACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAtctc > 2:112879/1‑140 (MQ=255) agaTACTAGGATGAGACATGCATAAAAACGCATAACTGCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAtctc < 1:398993/140‑1 (MQ=255) cTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACg > 1:536183/1‑128 (MQ=255) cTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACg < 2:536183/128‑1 (MQ=255) cTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCattat > 2:203286/1‑140 (MQ=255) tGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTgag > 2:495909/1‑140 (MQ=255) gAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTgaga > 2:411642/1‑140 (MQ=255) aGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCa > 2:503809/1‑123 (MQ=255) aGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCa < 1:503809/123‑1 (MQ=255) | TATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGA > NC_000913/584124‑584386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |