| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 584,320 | A→G | S214G (AGT→GGT) | appY → | DNA‑binding transcriptional activator AppY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 584,320 | 0 | A | G | 96.7% | 205.1 / ‑2.3 | 61 | S214G (AGT→GGT) | appY | DNA‑binding transcriptional activator AppY |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (30/29); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
AATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGTTTATTACAAGTTGTCTACAT > NC_000913/584184‑584456 | aataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTa < 1:427349/140‑1 (MQ=255) aGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTa < 1:742363/140‑1 (MQ=255) aGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTa < 2:647118/140‑1 (MQ=255) aGGAACGTCATTTACTGAAATAGTGAGAGATACTAGGGTGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTa < 1:336334/140‑1 (MQ=255) gAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTaaa < 1:607229/140‑1 (MQ=255) gAACGTCATTTACTGAAATATTAAGAGATCCTAGGATTAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTaaa < 1:317891/140‑1 (MQ=255) cGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGat > 2:546573/1‑140 (MQ=255) tGAAATATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGgtg < 1:150777/140‑1 (MQ=255) aaaTATTGAGAGATACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTc > 1:791629/1‑140 (MQ=255) tatTGAGAGATACTAGGATGAGGCAAGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACg < 1:372982/140‑1 (MQ=255) agagaTACTAGGATGAGACATGTAAAAAAACGCATTACTTCAAACTCTAATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAtc < 2:236086/140‑1 (MQ=255) agaTACTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAtctc > 2:112879/1‑140 (MQ=255) agaTACTAGGATGAGACATGCATAAAAACGCATAACTGCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAtctc < 1:398993/140‑1 (MQ=255) cTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACg < 2:536183/128‑1 (MQ=255) cTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACg > 1:536183/1‑128 (MQ=255) cTAGGATGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCattat > 2:203286/1‑140 (MQ=255) tGAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTgag > 2:495909/1‑140 (MQ=255) gAGGCATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTgaga > 2:411642/1‑140 (MQ=255) aggcaTGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGaa > 1:55479/5‑140 (MQ=255) aggcaTGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGaa > 1:63346/5‑140 (MQ=255) aggcaTGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGaa > 2:658195/5‑140 (MQ=255) aggcaTGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGaa > 2:178997/5‑140 (MQ=255) aGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCa < 1:503809/123‑1 (MQ=255) aGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCa > 2:503809/1‑123 (MQ=255) ggcaTGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATTGTGTCACGCCATCTCa > 1:601803/4‑127 (MQ=255) ggcaTGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATTGTGTCACGCCATCTCa < 2:601803/124‑1 (MQ=255) tGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCAATTAAAGATGATTGTGGGGTCACGCCATCTCATTATTTTGAGAAaataa > 2:240044/1‑140 (MQ=255) gCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAaataat > 1:7181/1‑140 (MQ=255) cAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATc > 1:127277/1‑140 (MQ=255) aaCTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTc < 2:7181/140‑1 (MQ=255) aaCTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTc < 1:178997/140‑1 (MQ=255) cATAACTTCAAACTCATATTCTATCCATGACGTAGCCGAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAg < 1:369487/140‑1 (MQ=255) aTAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGa > 1:726459/1‑140 (MQ=255) aTAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGa > 2:800250/1‑140 (MQ=255) cTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGa < 1:495909/140‑1 (MQ=255) cAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATa < 1:546573/140‑1 (MQ=255) aaCTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATaaa < 1:112879/140‑1 (MQ=255) ctctTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATaaaca < 2:726459/140‑1 (MQ=255) tctTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATaaacaa < 2:119193/140‑1 (MQ=255) ttATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAGCTCATTATGTTGTGAAAATAATCGGAGTCATAGATGGAATaaacaaaa > 2:224881/1‑140 (MQ=255) tATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATaaacaaaac > 2:479954/1‑140 (MQ=255) tATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTg < 2:63346/140‑1 (MQ=255) tATCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTg < 2:200607/140‑1 (MQ=255) tCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGAc > 2:642689/1‑140 (MQ=255) tCAATGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGAc > 2:559668/1‑140 (MQ=255) aaTGTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTg > 2:806451/1‑140 (MQ=255) gTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATa < 1:658195/140‑1 (MQ=255) gTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATa < 1:642689/140‑1 (MQ=255) gTCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATa < 1:479954/140‑1 (MQ=255) tCGTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATaa < 2:55479/140‑1 (MQ=255) gTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATg > 1:768899/1‑140 (MQ=255) gTAGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATg > 2:655003/1‑140 (MQ=255) aggCCCAGCAATGTGGCTATAACGGTACTTGTTGTTTCAAATGTGCATGTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGt < 2:382965/138‑1 (MQ=255) aGCCCAGAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGtt > 2:726607/1‑140 (MQ=255) gAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGTTTATTAc > 2:121749/1‑140 (MQ=255) gAAATGTGGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGTTTATTAc > 2:130057/1‑140 (MQ=255) tgGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAAGAATCGAGTGATAACGTTTATTACAAGtct > 1:382965/1‑138 (MQ=255) tgGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGTTTATTACAAGTTg > 2:504256/1‑140 (MQ=255) tgGCTATAACGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGTTTATTACAAGTTg > 2:765931/1‑140 (MQ=255) cTATAACGGTACTTCCTATTTCCTATGTGCATTTAAAAATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGTTTATTACAAGTTGTCt < 1:240044/140‑1 (MQ=255) aaCGGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGTTTATTACAAGTTGTCTACAt < 1:800250/140‑1 (MQ=255) | AATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACAATTGACTGATAATGTTTATTACAAGTTGTCTACAT > NC_000913/584184‑584456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |