| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 588,641 | A→G | Y772H (TAT→CAT) | nfrA ← | bacteriophage N4 receptor, outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 588,641 | 0 | A | G | 97.4% | 122.9 / ‑4.1 | 38 | Y772H (TAT→CAT) | nfrA | bacteriophage N4 receptor, outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/17); minor base T (1/0); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
TTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATAGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGCGACCGACCTCCTCATGCAAACGGCGGAAATTGAA > NC_000913/588511‑588773 | gagacaggCATCATCACCCCGTGTTCTCCGGTATCCGCAGAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTccc < 2:289476/134‑1 (MQ=255) catcatCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTg > 1:813132/1‑140 (MQ=255) caCCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGTTACGATGGCTTTTCCCTGGCGCTGcgccgc < 1:830414/140‑1 (MQ=255) caCCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGcgccgc < 1:666644/140‑1 (MQ=255) cccGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATTGCTTTTCCCTGGCGCTGCGCCGCCGa > 2:642435/1‑140 (MQ=255) cccGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGa > 1:616258/1‑140 (MQ=255) ccGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGGAGCTACGGTGGTTTGTCCCTGGCGCTGcgcctgtctc > 1:289476/1‑134 (MQ=255) gTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACat > 1:578735/1‑140 (MQ=255) ttttCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAACATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACatt > 1:187490/1‑140 (MQ=255) tcCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGt < 1:517159/140‑1 (MQ=255) tcCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGt < 2:616258/140‑1 (MQ=255) tcCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGt < 2:813132/140‑1 (MQ=255) tCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGt < 1:752498/140‑1 (MQ=255) aGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCAtt > 1:475894/1‑140 (MQ=255) taAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACg < 2:475894/140‑1 (MQ=255) cTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAg > 1:464904/1‑140 (MQ=255) agagCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCg > 2:205916/1‑140 (MQ=255) agagCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCg > 2:577900/1‑140 (MQ=255) agagCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCg > 1:106801/1‑140 (MQ=255) gagCAGGTCGCCTTCCAGCAGCCTATTGCGTGCGAAGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGa < 2:347592/140‑1 (MQ=255) gTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGgaag > 2:718644/1‑140 (MQ=255) cTTCCAGCAGCATATTGCGTCCGATGCGGTACTCAGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCg > 1:420/1‑140 (MQ=255) tatTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGTAAGCCGAGGGAAAAATCGAAACTGAACGTc > 2:380846/1‑140 (MQ=255) tatTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTc > 2:513176/1‑140 (MQ=255) ccGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAgcggcg > 1:182343/1‑140 (MQ=255) cGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGcga < 2:182343/140‑1 (MQ=255) cGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGcga < 2:187490/140‑1 (MQ=255) cGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGcga < 1:718644/140‑1 (MQ=255) tGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGcgaccg > 1:740637/1‑140 (MQ=255) tGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCCGAACGCAAGCCGATGGCAGAATCGACACTGAACGTCCAGCGGcgaccg > 2:257900/1‑140 (MQ=255) gCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGcgaccga < 2:740637/140‑1 (MQ=255) ggTACTCGGCTTCCAGTTGTCCGTATCTATGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGCgaccgacc < 1:380846/140‑1 (MQ=255) cTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGGCATTATTGTTAGCGGTACTCCTTGCGCCGGAACGCAAGCCGAAGGGAGAATCGAAAATGAACGACCACCGGCGACCGACCTCCTCactg > 2:743806/1‑137 (MQ=255) cTTCCAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGCGACCGACCTCCTCATGc > 2:824769/1‑140 (MQ=255) ttCCAGTTGTCCGTAGCTACGACGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGCGACCGACCTCCTCATGCa < 1:287771/140‑1 (MQ=255) ccAGTTGTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGCGACCGACCTCCTCATGCaaa > 2:589467/1‑140 (MQ=255) gTCCGTAGCTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTAAACCTCCAGCGGCGACAGACCCCCTCATGCAAAcggcgg > 2:287771/1‑140 (MQ=255) cTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGCGACCGACCTCCTCATGCAAACGGCGGAAATTGaa < 2:420/140‑1 (MQ=255) cTACGATGGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGCGACCGACCTCCTCATGCAAACGGCGGAAATTGaa < 1:792135/140‑1 (MQ=255) | TTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGTAGCTACGATAGCTTTTCCCTGGCGCTGCGCCGCCGACATTATTGTTAGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGCGACCGACCTCCTCATGCAAACGGCGGAAATTGAA > NC_000913/588511‑588773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |