Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,329	A→G	intergenic (‑3/+45)	umpH ← / ← nagC	UMP phosphatase/DNA‑binding transcriptional dual regulator NagC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,329	0	A	G	100.0%	134.3 / NA	38	intergenic (‑3/+45)	umpH/nagC	UMP phosphatase/DNA‑binding transcriptional dual regulator NagC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/18);  total (20/18)

GGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATC  >  NC_000913/700193‑700460                                                                                                                                         |                                                                                                                                    ggATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCAGCAGCACGCAGTCGATAGCGCAAATTACATTGTTAATGGTCATGGGCTa                                                                                                                                  >  2:213005/1‑140 (MQ=255)   aTAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTAcc                                                                                                                                >  1:143926/1‑140 (MQ=255)   aTAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTAcc                                                                                                                                <  1:741496/140‑1 (MQ=255)    tAGTTGGTCAGCAACTCCAGCGCCACGCCTTTATCCACAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTAccc                                                                                                                               <  1:213005/140‑1 (MQ=255)         cagcAGCAACACCAGCGGCAGGTCTTTATGCATAATCGCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAat                                                                                                                          <  1:396119/137‑1 (MQ=255)             aGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATAtt                                                                                                                       >  2:525683/1‑139 (MQ=255)             aGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATAtt                                                                                                                       <  1:525683/139‑1 (MQ=255)                     cAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAAt                                                                                                              >  2:182890/1‑140 (MQ=255)                       gCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCATATTACATTTTTACTGGCGATGGGCTACCCAAAATATTGAtaacaactg                                                                                                            >  2:396119/1‑137 (MQ=255)                        cGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAg                                                                                                           <  1:286701/140‑1 (MQ=255)                         ggCAGGCCTTTATCCATAATCCGGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAgc                                                                                                          <  1:333753/140‑1 (MQ=255)                           cAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAgcgc                                                                                                        <  2:143926/140‑1 (MQ=255)                           cAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAgcgc                                                                                                        <  1:540219/140‑1 (MQ=255)                                   tATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATaa                                                                                                >  1:556832/1‑140 (MQ=255)                                          aaTCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAgcg                                                                                                         <  2:687651/124‑1 (MQ=255)                                          aaTCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAgcg                                                                                                         >  1:687651/1‑124 (MQ=255)                                          aaTCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGcaca                                                                                         >  1:573160/1‑140 (MQ=255)                                          aaTCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGcaca                                                                                         >  1:10900/1‑140 (MQ=255)                                            tCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACAtt                                                                                       >  2:161085/1‑140 (MQ=255)                                                 tGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAATCGGCACTATAAAAGCACATTAAttt                                                                                  >  1:326447/1‑140 (MQ=255)                                                 tGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAAttt                                                                                  >  2:678561/1‑140 (MQ=255)                                                 tGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAAttt                                                                                  >  2:426129/1‑140 (MQ=255)                                                       aaTTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGc                                                                            <  2:654008/140‑1 (MQ=255)                                                          tCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCaaa                                                                         <  1:678561/140‑1 (MQ=255)                                                           ccGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAAt                                                                        >  2:590414/1‑140 (MQ=255)                                                             gCTGCCCCTCGTACGGCGACGTTATCGTGCATAAGACCGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAACAAGCGCCCCTACACAAGCACATTAATTCTCCAGCAAATGc                                                                      <  2:509966/140‑1 (MQ=255)                                                               tGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTg                                                                    <  1:182890/140‑1 (MQ=255)                                                                gCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTgg                                                                   <  1:590414/140‑1 (MQ=255)                                                                  aCCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAg                                                                 <  1:161085/140‑1 (MQ=255)                                                                      ggTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCaaa                                                             <  1:605298/140‑1 (MQ=255)                                                                       gTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCaaaa                                                            <  2:556832/140‑1 (MQ=255)                                                                             cGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGt                                                      >  2:16946/1‑140 (MQ=255)                                                                                cGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGa                                                   >  1:664288/1‑140 (MQ=255)                                                                                  ttATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGc                                                 >  1:20203/1‑140 (MQ=255)                                                                                                cACGCCGTCGATATCGCTAATTCCATTTTTAGTGGTCATAGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTAc                                   <  2:307471/140‑1 (MQ=255)                                                                                                              cGCAAATTACATTTTTAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAgcgc                     >  1:546397/1‑140 (MQ=255)                                                                                                                          ttttAATGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGc         >  1:110585/1‑140 (MQ=255)                                                                                                                               aTGGTCATGGGCTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGa    >  2:203070/1‑140 (MQ=255)                                                                                                                                 ggTCATGGGCTACCCTGAATATGGATAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATc  <  1:366574/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATC  >  NC_000913/700193‑700460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑