Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	53,721	A→G	F328L (TTC→CTC)	surA ←	peptidyl‑prolyl cis‑trans isomerase SurA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	53,721	0	A	G	100.0%	80.9 / NA	25	F328L (TTC→CTC)	surA	peptidyl‑prolyl cis‑trans isomerase SurA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/12);  total (13/12)

ACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAACTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCGACGGTTTCAGCAGAATATGGCGAGCATGAACTTCGGT  >  NC_000913/53597‑53856                                                                                                                             |                                                                                                                                        aCCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGgc                                                                                                                          >  1:748707/1‑140 (MQ=255)     gTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGgcagca                                                                                                                      >  1:681763/1‑140 (MQ=255)                tGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGtttt                                                                                                           <  2:681763/140‑1 (MQ=255)                    cTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGGGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCa                                                                                                       <  2:613128/140‑1 (MQ=255)                                   tCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGTCGGCAAAAGTCGTTTTAGCACTCTTGATATcagca                                                                                        >  2:379571/1‑140 (MQ=255)                                   tCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCa                                                                                                       <  1:503005/125‑1 (MQ=255)                                   tCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCa                                                                                                       >  2:503005/1‑125 (MQ=255)                                      gggCGTCACGGAAGGGCGGATCGAACATACCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTTCCACTCTTGATATCAgcggca                                                                                     <  1:317470/140‑1 (MQ=255)                                            cACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGt                                                                               <  1:519672/140‑1 (MQ=255)                                            cACGGAAGGCCGGATAGAAAATCTCAGGTTTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGt                                                                               <  1:382475/140‑1 (MQ=255)                                              cGGAAGGCCGGATCGTAAATATATGATGTAGCCCAGCAGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTc                                                                             <  1:379571/140‑1 (MQ=255)                                                                   tCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGGCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTc                                                        >  2:501163/1‑140 (MQ=255)                                                                        tgtAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCgtcag                                                   >  1:584527/1‑140 (MQ=255)                                                                                  ccGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCgg                                         >  1:803685/1‑140 (MQ=255)                                                                                  ccGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGAAGCAATCTATTCCAGTTTCACACGGTCCTGGTCGTCAGTCATGATCgg                                         >  2:348939/1‑140 (MQ=255)                                                                                    gagaTCGCCGCCCTGGTTAGCATAGGCTGCATCCTGACAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCg                                       <  1:287059/140‑1 (MQ=255)                                                                                    gagaTCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCg                                       <  1:762450/140‑1 (MQ=255)                                                                                            cgccCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCGACGGTTTc                               >  1:446892/1‑140 (MQ=255)                                                                                            cgccCTGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGACTGTTCGTGAGTCTTGATCGGCGACGGTTTc                               >  2:287059/1‑140 (MQ=255)                                                                                                 tGGTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCGACGGTTTcagcag                          <  1:501163/140‑1 (MQ=255)                                                                                                   gTTAGCAGAGCCTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCGACGGTTTCAGCAGaa                        >  2:174347/1‑140 (MQ=255)                                                                                                             ccTGGATCCTGAGAGAGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCGACGGTTTCAGCAGAATATGGCGAGc              >  1:206040/1‑140 (MQ=255)                                                                                                                       gagagaGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCGACGGTTTCAGCAGAATATGGCGAGCATGAACTTCg    >  1:822126/1‑140 (MQ=255)                                                                                                                         gagaGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCGACGGTTTCAGCAGAATATGGCGAGCATGAACTTCGGt  <  2:822126/140‑1 (MQ=255)                                                                                                                         gagaGCTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGACCTGTTCGTCAGTCATGATCGGCGACGGTTTCAGCAGAATATGGCGAGCATGAACTTCGGt  <  2:748707/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                        ACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCCCTGGTTAGCAGAGCCTGGATCCTGAGAGAACTCTTTCGCTGCGGCAGCAAAAGTCGTTTTACCACTCTTGATATCAGCAGCAATCTGTTCCAGTTTCACACGGGCCTGTTCGTCAGTCATGATCGGCGACGGTTTCAGCAGAATATGGCGAGCATGAACTTCGGT  >  NC_000913/53597‑53856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑