Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	790,895	T→C	L45L (TTA→TTG)	galT ←	galactose‑1‑phosphate uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	790,895	0	T	C	100.0%	82.6 / NA	25	L45L (TTA→TTG)	galT	galactose‑1‑phosphate uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/12);  total (13/12)

TCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGG  >  NC_000913/790758‑791031                                                                                                                                          |                                                                                                                                         tCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCaa                                                                                                                                        >  2:66694/1‑140 (MQ=255)   tGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAaca                                                                                                                                      >  1:200154/1‑140 (MQ=255)           cTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTtg                                                                                                                              >  2:533422/1‑140 (MQ=255)                              aGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGccc                                                                                                           <  1:533422/140‑1 (MQ=255)                                       gTCATTAAGCAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAgg                                                                                                  <  2:735992/140‑1 (MQ=255)                                                                gtgtAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAAc                                                                         >  1:523927/1‑140 (MQ=255)                                                                   tAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAg                                                                      <  1:487284/140‑1 (MQ=255)                                                                    aaTCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGa                                                                     >  1:541013/1‑140 (MQ=255)                                                                      tCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAAt                                                                   <  1:66694/140‑1 (MQ=255)                                                                             ttttATCGCCTGTCACCAGCACATTCCCTGCCCAGTGGATGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGc                                                            <  2:248577/140‑1 (MQ=255)                                                                                     ccTGTCACCCGCACATTACCTGCGCGGAGGAAGCAATGTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAg                                                    <  1:335374/140‑1 (MQ=255)                                                                                         tCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCggg                                                <  2:200154/140‑1 (MQ=255)                                                                                         tCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCCGTGCGGTGAAACCAAAATCCACTGCCCGGTGAGCggg                                                >  1:394207/1‑140 (MQ=255)                                                                                                cacaTTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAgc                                         >  2:417064/1‑140 (MQ=255)                                                                                                    ttACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATAGCCCGGTGAGCGGGTTGTAgcggcg                                     <  2:541013/140‑1 (MQ=255)                                                                                                           cgcAGAGGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGga                              >  1:94580/1‑140 (MQ=255)                                                                                                               gagGAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGgatgat                          >  1:574665/1‑140 (MQ=255)                                                                                                                  gAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCaa                       <  2:574665/140‑1 (MQ=255)                                                                                                                  gAAGCAATCTGGATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCaa                       <  2:94580/140‑1 (MQ=255)                                                                                                                    aGCAATCTGGATCGTGCTCAGGCAACACCTATTTGTCTGCCGTTTCCTCCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACg                     <  2:394207/140‑1 (MQ=255)                                                                                                                            ggATCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTaaa             >  1:193539/1‑140 (MQ=255)                                                                                                                              aTCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGa                  >  1:76895/1‑133 (MQ=255)                                                                                                                              aTCGTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGa                  <  2:76895/133‑1 (MQ=255)                                                                                                                                 gTGCGCAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCg        >  2:71045/1‑140 (MQ=255)                                                                                                                                    cgcAGGCAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGAT‑‑‑CGGGATTAAATTGCGTCATgg  >  1:115852/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGG  >  NC_000913/790758‑791031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑