Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	824,547	C→T	intergenic (‑50/‑83)	ybhP ← / → ybhQ	endonuclease/exonuclease/phosphatase domain‑containing protein YbhP/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	824,547	0	C	T	100.0%	160.3 / NA	48	intergenic (‑50/‑83)	ybhP/ybhQ	endonuclease/exonuclease/phosphatase domain‑containing protein YbhP/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/26);  total (22/26)

AATGAAGCGTCGGTTAAACGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATT  >  NC_000913/824411‑824683                                                                                                                                         |                                                                                                                                         aaTGAAGCGTCGGTTAAACGCGGTAAAGCCTTTGTGAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGGGTAGTCGGCGTtaca                                                                                                                                       >  1:240482/1‑140 (MQ=255)        cGTCGGTTAAACGCGGTAAAGCCTTTGTGAATATTGATGGCGAGCACCTTGAACTAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGt                                                                                                                                >  2:553146/1‑140 (MQ=255)         gTCGGTTAAACGCGGTAAAGCCTTTGTGAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTg                                                                                                                               <  2:775725/140‑1 (MQ=255)                      ggTAAAGCCTTTGTGAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGGTAAAAAAAGTTGCGGTCTTACGGaa                                                                                                                  >  1:319136/1‑140 (MQ=255)                        tAAAGCCTTTGTGAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAAtt                                                                                                                >  1:410989/1‑140 (MQ=255)                        tAAAGCCTTTGTGAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTATAGTCGGAGTTACAAAAAGGTACGGTCTTACGGAAtt                                                                                                                >  1:304992/1‑140 (MQ=255)                               tttGTGAATATTGATGGGGAGCGCCTTGAACGAAATTTGCTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTa                                                                                                         <  2:327600/140‑1 (MQ=255)                               tttGTGAATATTGATGGCGAGCTCCTTGAACCAAATTTGCTGTGTTTGGTCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTa                                                                                                         <  2:319136/140‑1 (MQ=255)                               tttGTGAATATTGATGGCGAGCCCCTTGAACGAAAATTGATGTGTTTGCTCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTa                                                                                                         <  2:240482/140‑1 (MQ=255)                               tttGTGAATATTGATGGCGAGCACCTTGAACTAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTa                                                                                                         <  1:553146/140‑1 (MQ=255)                               tttGTGAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTa                                                                                                         <  2:410989/140‑1 (MQ=255)                                ttGTGAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaa                                                                                                        >  2:597812/1‑140 (MQ=255)                                 tgtgAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaaa                                                                                                       <  2:591882/140‑1 (MQ=255)                                 tgtgAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaaa                                                                                                       >  2:123486/1‑140 (MQ=255)                                 tgtgAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTGTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGTAATTTTCCGTaat                                                                                                       >  1:682081/1‑139 (MQ=255)                                  gtgAATATTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAg                                                                                                      >  2:723664/1‑140 (MQ=255)                                      atatTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCg                                                                                                  <  1:597812/140‑1 (MQ=255)                                       aatTGATGGCGAGCACCTTGATCGAAAATTGTTGTGTCTGATCGGGCATGATGTTCCTGTCTTTGCCTCTTATCTGATTGAAATAGTGTAGACGGCGTTCCAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCgg                                                                                                 <  2:682081/139‑1 (MQ=255)                                        atTGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGt                                                                                                >  2:434518/1‑140 (MQ=255)                                          tGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTAc                                                                                              <  1:130353/140‑1 (MQ=255)                                          tGATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTAc                                                                                              <  1:690314/140‑1 (MQ=255)                                           gATGGCGAGCACCTTGAACGAATATTGTTGTCTTTTATCCGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTAct                                                                                             <  2:306716/140‑1 (MQ=255)                                           gATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTAct                                                                                             <  2:525762/140‑1 (MQ=255)                                           gATGGCGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTAct                                                                                             <  1:434518/140‑1 (MQ=255)                                                cgAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActc                                                                                            <  1:716438/135‑1 (MQ=255)                                                cgAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActc                                                                                            >  2:716438/2‑136 (MQ=255)                                                  aGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActc                                                                                            <  1:723357/134‑1 (MQ=255)                                                  aGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActc                                                                                            >  2:723357/1‑134 (MQ=255)                                                  aGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGGCTTACGGGATTGTCAGTAAAGTTCGGTACTCTGagta                                                                                      >  1:258335/1‑140 (MQ=255)                                                      ccTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaagta                                                                                  <  1:621322/140‑1 (MQ=255)                                                      ccTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaagta                                                                                  <  2:84541/140‑1 (MQ=255)                                                      ccTTGAACGAAAATTGTTGTGTGTGTTCGCGCATAATCTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaagta                                                                                  <  1:285335/140‑1 (MQ=255)                                                         tGAACGAAAATTGTTTTGTTTGGTCGGGCGTAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTagag                                                                               <  1:390689/140‑1 (MQ=255)                                                         tGAACGAAAATTGGTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTagag                                                                               >  2:791095/1‑140 (MQ=255)                                                                 aaTTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAttc                                                                       >  1:160569/1‑140 (MQ=255)                                                                   ttgttgTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAttctt                                                                     <  2:160569/140‑1 (MQ=255)                                                                   ttgttgTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAttctt                                                                     <  2:651796/140‑1 (MQ=255)                                                                                   ggCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATg                                                     >  1:427259/1‑140 (MQ=255)                                                                                                  tCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACgt                                      >  2:43243/1‑140 (MQ=255)                                                                                                              aTCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCa                          >  2:407170/1‑140 (MQ=255)                                                                                                              aTCTCATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCa                          >  2:110180/1‑140 (MQ=255)                                                                                                               tctcATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCaa                         <  1:43243/140‑1 (MQ=255)                                                                                                                 tcATTGAAATAGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACg                       <  1:407170/140‑1 (MQ=255)                                                                                                                           aGTGTAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGtt             >  1:821496/1‑140 (MQ=255)                                                                                                                            gtgtAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTg            <  1:791095/140‑1 (MQ=255)                                                                                                                               tAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTg         >  2:504217/1‑140 (MQ=255)                                                                                                                               tAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTg         >  2:29547/1‑140 (MQ=255)                                                                                                                               tAGTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTg         >  2:743406/1‑140 (MQ=255)                                                                                                                                 gTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCt          >  2:109317/1‑137 (MQ=255)                                                                                                                                 gTCGGCGTTACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCt          <  1:109317/137‑1 (MQ=255)                                                                                                                                      cGTTACAAAAAGGTGCGCTCTTACGGAATTTACCGGAAACTTCGGTACGCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGAtt  <  2:258335/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AATGAAGCGTCGGTTAAACGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATT  >  NC_000913/824411‑824683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑