Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	833,631	A→G	S188G (AGT→GGT)	dinG →	ATP‑dependent DNA helicase DinG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	833,631	0	A	G	100.0%	92.8 / NA	28	S188G (AGT→GGT)	dinG	ATP‑dependent DNA helicase DinG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/11);  total (17/11)

AGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCAAACCATGCGCTGGTGATGGCGGCGATGGAAA  >  NC_000913/833522‑833766                                                                                                              |                                                                                                                                        agagCAAAAACGTTGTGCGAAGTTGAAGGGCCATCACGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCa                                                                                                           <  2:292140/140‑1 (MQ=255) agagCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATCCTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCa                                                                                                           <  2:530507/140‑1 (MQ=255) agagCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTTTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCa                                                                                                           <  2:95049/140‑1 (MQ=255)  gagCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCaa                                                                                                          <  2:25574/140‑1 (MQ=255)   agCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAAt                                                                                                         >  1:778518/1‑140 (MQ=255)                          aaGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtac                                                                                                   >  2:778521/1‑123 (MQ=255)                          aaGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtac                                                                                                   <  1:778521/123‑1 (MQ=255)                                       aTACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTTCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCt                                                                     >  2:620243/1‑140 (MQ=255)                                                     gATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATAGTGAATGGCCGCTTCTTGTCGATCGTCGGGAGATTCa                                                       >  1:218855/1‑140 (MQ=255)                                                               gcgATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGaa                                             >  2:809335/1‑140 (MQ=255)                                                                   tCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAgtgg                                         >  2:600205/1‑140 (MQ=255)                                                                             atCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCaaa                               >  2:804277/1‑140 (MQ=255)                                                                             atCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCaaa                               >  1:43837/1‑140 (MQ=255)                                                                             atCGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCaaa                               >  2:588562/1‑140 (MQ=255)                                                                               cGCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCAAAcc                             <  1:804277/140‑1 (MQ=255)                                                                                gCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGc                                  <  2:34288/134‑1 (MQ=255)                                                                                gCTATAGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGc                                  >  1:34288/1‑134 (MQ=255)                                                                                   ataGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCAAACCATgc                         >  2:166065/1‑140 (MQ=255)                                                                                     aGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGGTTCAGGAAGCGGAAGTGGTGGATGCAAACCATgcgc                       >  2:711308/1‑140 (MQ=255)                                                                                     aGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGc                                  <  2:161946/129‑1 (MQ=255)                                                                                     aGATGACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGc                                  >  1:161946/1‑129 (MQ=255)                                                                                         gACGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCAAACCATGCGCTGGt                   <  1:588562/140‑1 (MQ=255)                                                                                          aCGATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGACGGGAGATCCAGAACGCGGAAGTGGGGGTGCAAAACCATGCGCTGGTg                  >  2:373451/1‑140 (MQ=255)                                                                                            gATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCAAACCATGCGCTGGTGAt                <  1:600205/140‑1 (MQ=255)                                                                                            gATCTCTGGCGTCGTTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGATGGCAACCCATGCGCTGGTGAt                >  1:294949/1‑140 (MQ=255)                                                                                              tctcTGGCGTCGTTTAGTTACCTACAAAGCCAGCTACCTCAACCGCAAGTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCAAACCATGCGCTGGTGATgg              <  2:294949/140‑1 (MQ=255)                                                                                                         gtTTAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCAAACCATGCGCTGGTGATGGCGGCGATGGaa   >  2:777984/1‑140 (MQ=255)                                                                                                          tttAGGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCAAACCATGCGCTGGTGATGGCGGCGATGGaaa  >  2:533668/1‑140 (MQ=255)                                                                                                              |                                                                                                                                        AGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGTCGGGAGATTCAGGAAGCGGAAGTGGTGGTGGCAAACCATGCGCTGGTGATGGCGGCGATGGAAA  >  NC_000913/833522‑833766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑