Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	840,048	A→G	V495A (GTG→GCG)	fiu ←	putative iron siderophore outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	840,048	0	A	G	100.0%	171.3 / NA	52	V495A (GTG→GCG)	fiu	putative iron siderophore outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/24);  total (28/24)

AGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCACACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATATTCAGTATGATAATTATCCAGACGGATCCCGCCGTTCAGCTCAAAATCACGGGTGATTTGCAGCGTATCAAA  >  NC_000913/839915‑840181                                                                                                                                      |                                                                                                                                      aGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAg                                                                                                                                 <  1:137669/140‑1 (MQ=255)      gctgGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTggg                                                                                                                            <  2:646906/140‑1 (MQ=255)                    tAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAAAACCTTTGGCCGCAGCAGTTGAGCAGGTGATGGCAcc                                                                                                              >  1:220650/1‑140 (MQ=255)                    tAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGCGGTGACCGGAGACCCTTTTGCCGCAACAGTTGGGCAGGTGATGGCAcc                                                                                                              >  2:359180/1‑140 (MQ=255)                            ataGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTGGCCGCACTAGTTGGGCAGGTGCTGGCACCGCGTCCGc                                                                                                      >  1:263060/1‑140 (MQ=255)                              aGACATTGCCGTTTTCCGTCAGGTGTTACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTg                                                                                                    <  1:359180/140‑1 (MQ=255)                               gACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTgc                                                                                                   >  2:137090/1‑140 (MQ=255)                                aCATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGCACCTTTTGCCGCGCCAGTTGGGCAGTTGATCGCACCGCGTCCGCTgcc                                                                                                  >  2:240637/1‑140 (MQ=255)                                aCATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTgcc                                                                                                  >  2:534953/1‑140 (MQ=255)                                aCATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTgcc                                                                                                  >  2:144693/1‑140 (MQ=255)                                aCATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTgcc                                                                                                  >  1:568957/1‑140 (MQ=255)                                aCATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTgcc                                                                                                  >  1:48582/1‑140 (MQ=255)                                 cATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTgccg                                                                                                 >  1:84686/1‑140 (MQ=255)                                 cATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTgccg                                                                                                 >  1:75253/1‑140 (MQ=255)                                 cATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTgccg                                                                                                 >  1:293590/1‑140 (MQ=255)                                    tGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGccgccg                                                                                              >  2:589531/1‑140 (MQ=255)                                    tGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGccgccg                                                                                              >  1:32426/1‑140 (MQ=255)                                    tGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCATTTGCCGCACCAGTTGTGCAGGTGATGGCATCGCGTCCGCTGccgccg                                                                                              >  2:273285/1‑140 (MQ=255)                                     gCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCAGCAGTTGGGCAGGTGATTGCACAGCGTCCGCTGAcgccgc                                                                                             >  2:249349/1‑140 (MQ=255)                                     gCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCcgccgc                                                                                             >  1:493447/1‑140 (MQ=255)                                          tttCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCTGCACCAGTTGGGGAGGAGATGGCACAGCGTGCGCTGCCGCCGCAGGCg                                                                                        >  2:286165/1‑140 (MQ=255)                                           ttCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCgg                                                                                       <  1:788690/140‑1 (MQ=255)                                           ttCCGTCAGGTGATACAACGCCACGGCTTTCCAGTTCATAAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCgg                                                                                       <  2:263060/140‑1 (MQ=255)                                              cGTCAGGTGATACAGCGCCCCGTCTCTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTgg                                                                                    <  1:240637/140‑1 (MQ=255)                                              cGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTgg                                                                                    <  2:32426/140‑1 (MQ=255)                                               gTCAGGTGATACAGCGCCCCGGCTTTCCAGTGCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGc                                                                                   <  2:293590/140‑1 (MQ=255)                                                tCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAgg                                                                                          >  1:830329/1‑132 (MQ=255)                                                tCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAgg                                                                                          <  2:830329/132‑1 (MQ=255)                                                tCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  2:84686/140‑1 (MQ=255)                                                tCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  2:48582/140‑1 (MQ=255)                                                tCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  2:568957/140‑1 (MQ=255)                                                tCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  1:541779/140‑1 (MQ=255)                                                tCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  1:557185/140‑1 (MQ=255)                                                tCAGGTGATACAGCGCCCCGGCATTGCAGTTCATCAGGTTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  1:286165/140‑1 (MQ=255)                                                tCAGGTGATACAGCGCCCCGGCATTCCAGTTCATCTGATAGCCCGACTAGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  2:220650/140‑1 (MQ=255)                                                tCAGGTGATACAGCGACCCGGCATTACAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  1:377106/140‑1 (MQ=255)                                                     tGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTc                                                                             <  1:137090/140‑1 (MQ=255)                                                        tACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCata                                                                          <  1:589531/140‑1 (MQ=255)                                                          cAGCGCCCCGGCTTTCCAGTTCCTCCGATTGCCCGACATGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATAtt                                                                        <  1:273285/140‑1 (MQ=255)                                                             cgcCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAGCCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  2:39806/127‑1 (MQ=255)                                                             cgcCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  1:77168/127‑1 (MQ=255)                                                             cgcCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  >  2:77168/1‑127 (MQ=255)                                                             cgcCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  >  1:39806/1‑127 (MQ=255)                                                              gcCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCACCGCAGGCGGTGGAACTGTCATATTCAGt                                                                    >  1:328938/1‑140 (MQ=255)                                                               ccccGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATATTCAGTa                                                                   >  1:509703/1‑140 (MQ=255)                                                                 ccGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATATTCAGTATg                                                                 >  1:435300/1‑140 (MQ=255)                                                                  cGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  <  1:742000/122‑1 (MQ=255)                                                                  cGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCa                                                                                  >  2:742000/1‑122 (MQ=255)                                                                               catcaGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATATTCAGTATGATAATTATCCAGAc                                                   >  2:459923/1‑140 (MQ=255)                                                                                  caGATGGCCCGTTTGGGCGGGGTGGGCGGTGGTGACCGGAGACCCTTTGGCCTCACCATTTGGGCGGGTGATGCCACCGCGTCCGCGGCCGCCGCAGGCGGTGGCATTGTAATATTCATTATGATAATTATCCAGACGGa                                                <  2:192997/140‑1 (MQ=255)                                                                                         cccGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGGCATATTCAGTATGATAATTATCCAGACGGATCccgcc                                         >  1:192997/1‑140 (MQ=255)                                                                                                                       ggAGAGCCTTTTGCCGCACCAGAGGGGCAGGAGAGCGCACCGCGTCCCCTGCCGCCGCAGGCGGTGGCACTGTCATATTCAGTATGATAATTATCCAGACGGATCCCGCCGTTCAGCTCAAAATCACGGGTGATTTGCAg           <  2:313323/140‑1 (MQ=255)                                                                                                                           aaCCTTTTGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATATTCAGTATGATAATTATCTAGACGGATTCCGGCGTCCAGCTCAAAATCACGGGCGATTTGCAGCGTa       >  2:302686/1‑140 (MQ=255)                                                                                                                                tttGCCGCACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATATTCAGTATGATAATTATCCAGACGGATCCCGCCGTTCAGCTCAAAATCACGGGTGATTTGCAGCGTATCaaa  <  2:493447/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      AGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCACACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATATTCAGTATGATAATTATCCAGACGGATCCCGCCGTTCAGCTCAAAATCACGGGTGATTTGCAGCGTATCAAA  >  NC_000913/839915‑840181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑