Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	59,658	A→G	intergenic (+379/+29)	yabQ → / ← rluA	protein YabQ/23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	59,658	0	A	G	100.0%	133.1 / NA	38	intergenic (+379/+29)	yabQ/rluA	protein YabQ/23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/23);  total (15/23)

AAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCT  >  NC_000913/59523‑59788                                                                                                                                        |                                                                                                                                   aaaGTTCAAAAATTCGCTGTGGCGCAGGAAGTGAAGTACTGAATGGAACGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGAt                                                                                                                                <  2:247690/140‑1 (MQ=255) aaaGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGAt                                                                                                                                <  1:175184/140‑1 (MQ=255) aaaGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGAt                                                                                                                                <  1:715220/140‑1 (MQ=255) aaaGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGAt                                                                                                                                <  1:579334/140‑1 (MQ=255)         aaaaTTCACTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGtat                                                                                                                        <  1:616227/140‑1 (MQ=255)                      gAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGaa                                                                                                           >  2:746730/1‑140 (MQ=255)                                 gAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAAt                                                                                                >  2:15727/1‑140 (MQ=255)                                      aCCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGa                                                                                                            <  2:597941/123‑1 (MQ=255)                                      aCCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGa                                                                                                            >  1:597941/1‑123 (MQ=255)                                            tGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAAAAAAAAATCCCCGGACTCGGGGATTTATGAACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCt                                                                                     >  1:693914/1‑140 (MQ=255)                                               aaaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTa                                                                                  <  1:746730/140‑1 (MQ=255)                                                aaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTaa                                                                                 <  2:54697/140‑1 (MQ=255)                                                aaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTaa                                                                                 <  2:5180/140‑1 (MQ=255)                                                aaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTaa                                                                                 <  2:564784/140‑1 (MQ=255)                                                aaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTaa                                                                                 <  1:354276/140‑1 (MQ=255)                                                 aGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTaaa                                                                                <  1:458283/140‑1 (MQ=255)                                                   cGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACg                                                                              <  2:599498/140‑1 (MQ=255)                                                    gTAGCCACACGCAACAAATGAATGCAGTTTGGCAGTAACGAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGt                                                                             <  2:296092/140‑1 (MQ=255)                                                       gCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACTGGAAAGGATAACAAACCGCTGGCGCTTTAAACGTCAt                                                                          >  1:296092/1‑140 (MQ=255)                                                            acGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTAt                                                                     <  1:758938/140‑1 (MQ=255)                                                             cGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATGCTAtt                                                                    >  2:997/1‑140 (MQ=255)                                                                  caacTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCAt                                                               >  2:96249/1‑140 (MQ=255)                                                                               tttGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGt                                                  >  1:127558/1‑140 (MQ=255)                                                                               tttGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGt                                                  >  2:808109/1‑140 (MQ=255)                                                                                 tGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTaa                                                <  2:127558/140‑1 (MQ=255)                                                                                  ggCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAAt                                               >  1:205623/1‑140 (MQ=255)                                                                                          cAAAAAATCCCCGGACTCGGGGCTTAATGCTCAAGAGCCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTTGCGCTTTAAACGTCATTCTATTGCCATACGCCGGATGGGTAATCGTCAACa                                       <  1:247429/140‑1 (MQ=255)                                                                                           aaaaaaTCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACAt                                      <  1:96249/140‑1 (MQ=255)                                                                                                   cccGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCAt                              <  1:566381/140‑1 (MQ=255)                                                                                                        aCTCGGGGATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGc                         <  1:324435/140‑1 (MQ=255)                                                                                                            ggggATTTATGTACAAGAGGCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGcaaca                     <  2:205623/140‑1 (MQ=255)                                                                                                                               gCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACTTCTCTGCATGTTGCAACAAACTTGGTGCCATCGctct  >  1:294047/1‑140 (MQ=255)                                                                                                                               gCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGctct  >  1:490378/1‑140 (MQ=255)                                                                                                                               gCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGct    >  2:5116/1‑138 (MQ=255)                                                                                                                               gCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGct    >  2:34297/1‑138 (MQ=255)                                                                                                                               gCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGct    <  1:34297/138‑1 (MQ=255)                                                                                                                               gCAGCCCTTGGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGct    <  1:5116/138‑1 (MQ=255)                                                                                                                               gCAGCCCTTGGGATGAGGGGATAAACGTACAGGAAAGGGTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACCTGGTGCCATCGctct  >  1:371648/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   AAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCT  >  NC_000913/59523‑59788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑